Distinguishing between soil sources of N2O using a new 15N- and 18O-enrichment method

N. Wrage, J. W. van Groeningen, O. Oenema, Elizabeth Baggs

Research output: Contribution to journalArticle

127 Citations (Scopus)

Abstract

We present a novel O-18-N-15-enrichment method for the distinction between nitrous oxide (N2O) from nitrification, nitrifier denitrification and denitrification based on a method with single- and double-N-15-labelled ammonium nitrate. We added a new treatment with O-18-labelled water to quantify N2O from nitrifier denitrification. The theory behind this is that ammonia oxidisers use oxygen (O-2) from soil air for the oxidation of ammonia (NH3) but use H2O for the oxidation of the resulting hydroxylamine (NH2OH) to nitrite (NO2-). Thus, N2O from nitrification would therefore be expected to reflect the O-18 signature of soil O-2, whereas the O-18 signature of N2O from nitrifier denitrification would reflect that of both soil O-2 and H2O. It was assumed that (a) there would be no preferential removal of O-18 or O-16 during nitrifier denitrification or denitrification, (b) the O-18 signature of the applied O-18-labelled water would remain constant over the experimental period, and (c) any O exchange between (H2O)-O-18 and NO3- would be negligible under the chosen experimental conditions. These assumptions were tested and validated for a silt loam soil at 50% water-filled pore space (WFPS) following application of 400 mg N kg(-1) dry soil. We compared the results of our new method with those of a conventional inhibition method using 0.02% v/v acetylene (C2H2) and 80% v/v O-2 in helium. Both the O-18-N-15-enrichment and inhibitor methods identified nitrifter denitrification to be a major source of N2O, accounting for 44 and 40%, respectively, of N2O production over 24 h. However, compared to our O-18-N-15-method, the inhibitor method overestimated the contribution from nitrification at the expense of denitrification, probably due to incomplete inhibition of nitrifier denitrification and denitrification by large concentrations Of 02 and a negative effect of C2H2 on denitrification. We consider our new O-18-N-15-enrichment method to be more reliable than the use of inhibitors; it enables the distinction between more soil sources of N2O than was previously possible and has provided the first direct evidence of the significance of nitrifier denitrification as a source of N2O in fertilised arable soil. Copyright (c) 2005 John Wiley & Sons, Ltd.

Original languageEnglish
Pages (from-to)3298-3306
Number of pages8
JournalRapid Communications in Mass Spectrometry
Volume19
DOIs
Publication statusPublished - 2005

Keywords

  • NITROUS-OXIDE PRODUCTION
  • NITRIFIER DENITRIFICATION
  • NITROSOMONAS-EUROPAEA
  • NITRIFICATION
  • N-15
  • NITRITE
  • INHIBITION
  • ACETYLENE
  • REDUCTION
  • GRASSLAND

Cite this

Distinguishing between soil sources of N2O using a new 15N- and 18O-enrichment method. / Wrage, N.; van Groeningen, J. W.; Oenema, O.; Baggs, Elizabeth.

In: Rapid Communications in Mass Spectrometry, Vol. 19, 2005, p. 3298-3306.

Research output: Contribution to journalArticle

Wrage, N. ; van Groeningen, J. W. ; Oenema, O. ; Baggs, Elizabeth. / Distinguishing between soil sources of N2O using a new 15N- and 18O-enrichment method. In: Rapid Communications in Mass Spectrometry. 2005 ; Vol. 19. pp. 3298-3306.
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T1 - Distinguishing between soil sources of N2O using a new 15N- and 18O-enrichment method

AU - Wrage, N.

AU - van Groeningen, J. W.

AU - Oenema, O.

AU - Baggs, Elizabeth

PY - 2005

Y1 - 2005

N2 - We present a novel O-18-N-15-enrichment method for the distinction between nitrous oxide (N2O) from nitrification, nitrifier denitrification and denitrification based on a method with single- and double-N-15-labelled ammonium nitrate. We added a new treatment with O-18-labelled water to quantify N2O from nitrifier denitrification. The theory behind this is that ammonia oxidisers use oxygen (O-2) from soil air for the oxidation of ammonia (NH3) but use H2O for the oxidation of the resulting hydroxylamine (NH2OH) to nitrite (NO2-). Thus, N2O from nitrification would therefore be expected to reflect the O-18 signature of soil O-2, whereas the O-18 signature of N2O from nitrifier denitrification would reflect that of both soil O-2 and H2O. It was assumed that (a) there would be no preferential removal of O-18 or O-16 during nitrifier denitrification or denitrification, (b) the O-18 signature of the applied O-18-labelled water would remain constant over the experimental period, and (c) any O exchange between (H2O)-O-18 and NO3- would be negligible under the chosen experimental conditions. These assumptions were tested and validated for a silt loam soil at 50% water-filled pore space (WFPS) following application of 400 mg N kg(-1) dry soil. We compared the results of our new method with those of a conventional inhibition method using 0.02% v/v acetylene (C2H2) and 80% v/v O-2 in helium. Both the O-18-N-15-enrichment and inhibitor methods identified nitrifter denitrification to be a major source of N2O, accounting for 44 and 40%, respectively, of N2O production over 24 h. However, compared to our O-18-N-15-method, the inhibitor method overestimated the contribution from nitrification at the expense of denitrification, probably due to incomplete inhibition of nitrifier denitrification and denitrification by large concentrations Of 02 and a negative effect of C2H2 on denitrification. We consider our new O-18-N-15-enrichment method to be more reliable than the use of inhibitors; it enables the distinction between more soil sources of N2O than was previously possible and has provided the first direct evidence of the significance of nitrifier denitrification as a source of N2O in fertilised arable soil. Copyright (c) 2005 John Wiley & Sons, Ltd.

AB - We present a novel O-18-N-15-enrichment method for the distinction between nitrous oxide (N2O) from nitrification, nitrifier denitrification and denitrification based on a method with single- and double-N-15-labelled ammonium nitrate. We added a new treatment with O-18-labelled water to quantify N2O from nitrifier denitrification. The theory behind this is that ammonia oxidisers use oxygen (O-2) from soil air for the oxidation of ammonia (NH3) but use H2O for the oxidation of the resulting hydroxylamine (NH2OH) to nitrite (NO2-). Thus, N2O from nitrification would therefore be expected to reflect the O-18 signature of soil O-2, whereas the O-18 signature of N2O from nitrifier denitrification would reflect that of both soil O-2 and H2O. It was assumed that (a) there would be no preferential removal of O-18 or O-16 during nitrifier denitrification or denitrification, (b) the O-18 signature of the applied O-18-labelled water would remain constant over the experimental period, and (c) any O exchange between (H2O)-O-18 and NO3- would be negligible under the chosen experimental conditions. These assumptions were tested and validated for a silt loam soil at 50% water-filled pore space (WFPS) following application of 400 mg N kg(-1) dry soil. We compared the results of our new method with those of a conventional inhibition method using 0.02% v/v acetylene (C2H2) and 80% v/v O-2 in helium. Both the O-18-N-15-enrichment and inhibitor methods identified nitrifter denitrification to be a major source of N2O, accounting for 44 and 40%, respectively, of N2O production over 24 h. However, compared to our O-18-N-15-method, the inhibitor method overestimated the contribution from nitrification at the expense of denitrification, probably due to incomplete inhibition of nitrifier denitrification and denitrification by large concentrations Of 02 and a negative effect of C2H2 on denitrification. We consider our new O-18-N-15-enrichment method to be more reliable than the use of inhibitors; it enables the distinction between more soil sources of N2O than was previously possible and has provided the first direct evidence of the significance of nitrifier denitrification as a source of N2O in fertilised arable soil. Copyright (c) 2005 John Wiley & Sons, Ltd.

KW - NITROUS-OXIDE PRODUCTION

KW - NITRIFIER DENITRIFICATION

KW - NITROSOMONAS-EUROPAEA

KW - NITRIFICATION

KW - N-15

KW - NITRITE

KW - INHIBITION

KW - ACETYLENE

KW - REDUCTION

KW - GRASSLAND

U2 - 10.1002/rcm.2191

DO - 10.1002/rcm.2191

M3 - Article

VL - 19

SP - 3298

EP - 3306

JO - Rapid Communications in Mass Spectrometry

JF - Rapid Communications in Mass Spectrometry

SN - 0951-4198

ER -