Distribution and genetic diversity of Dothistroma septosporum in Pinus brutia forests of south-western Turkey

F. Oskay* (Corresponding Author), Z. Tunali, A. Lehtijärvi, H. T. Doğmuş Lehtijärvi, S. Woodward, M. Mullett

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

Dothistroma needle blight (DNB) is a serious disease of the Pinaceae, mainly Pinus species, caused by the fungi Dothistroma septosporum and D. pini. Both species are regarded as invasive forest pathogens worldwide, with rising incidence in central and northern Europe over the last three decades. In this work, 29 sites were investigated between 2013 and 2015 in south-western Turkey. Morphological examination of needles confirmed DNB infection (i.e., Dothistroma conidiospores observed) at 18 sites, and a total of 108 Dothistroma sp. isolates were obtained from 11 of the sites. Host age seemed to be an important factor in both occurrence and severity of DNB in Pinus brutia forests. Continuous rainy days, especially in December, may increase severity of disease; however, extreme rain events may reduce available conidiospores on plant tissues or in the air. Species-specific mating type primers showed that all isolates were D. septosporum; D. pini was not detected. The mating type ratio was close to 1:1, indicating sexual recombination was occurring. Eleven microsatellite markers revealed 59 unique multilocus haplotypes (MLHs) among the 73 isolates originating from different conidiomata. The majority of MLHs were represented by a single isolate (n = 52) and only one MLH was shared between two localities. Analyses showed high genetic diversity, isolation-by-distance, and clear population clusters. These findings suggest that D. septosporum is well established in south-western Turkey and is probably not a recent introduction.

Original languageEnglish
Pages (from-to)1551-1564
Number of pages14
JournalPlant Pathology
Volume69
Issue number8
Early online date10 Aug 2020
DOIs
Publication statusE-pub ahead of print - 10 Aug 2020

Keywords

  • Red band needle blight
  • Dothistroma septosporum
  • Population genetics
  • microsatellites
  • SSR
  • Mediterranean pine forests
  • invasive forest pathogens (IFPs)
  • population genetics
  • red band needle blight
  • invasive forest pathogens
  • PATHOGEN
  • HOST-RANGE
  • PATTERNS
  • INFERENCE
  • DOTHISTROMA NEEDLE BLIGHT
  • MICROSATELLITE MARKERS
  • POPULATION-STRUCTURE
  • PINUS

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