Distribution of Tetracycline and Erythromycin Resistance Genes Among Human Oral and Fecal Metagenomic DNA

Lorna A. Seville; Andrea J. Patterson; Karen Patricia Scott; Peter Mullany; Michael A. Quail; Julian Parkhill; Derren Ready; Michael Wilson; David Spratt; Adam P. Roberts

doi:10.1089/mdr.2009.0916

Distribution of Tetracycline and Erythromycin Resistance Genes Among Human Oral and Fecal Metagenomic DNA

Lorna A. Seville, Andrea J. Patterson, Karen Patricia Scott, Peter Mullany, Michael A. Quail, Julian Parkhill, Derren Ready, Michael Wilson, David Spratt, Adam P. Roberts

The Rowett Institute of Nutrition and Health

Wellcome Sanger Institute

Research output: Contribution to journal › Article › peer-review

93 Citations (Scopus)

Abstract

We have analyzed the total metagenomic DNA from both human oral and fecal samples derived from healthy volunteers from six European countries to determine the molecular basis for tetracycline and erythromycin resistance. We have determined that tet(M) and tet(W) are the most prevalent tetracycline resistance genes assayed for in the oral and fecal metagenomes, respectively. Additionally, tet(Q), tet(O), and tet(O/32/O) have been shown to be common. We have also shown that erm(B), erm(V), and erm(E) are common erythromycin resistance genes present in these environments. Further, we have demonstrated the ubiquitous presence of the Tn916 integrase in the oral metagenomes and the Tn4451 and Tn1549 integrase genes within the fecal metagenomes.</p>

Original language	English
Pages (from-to)	159-166
Number of pages	8
Journal	Microbial Drug Resistance
Volume	15
Issue number	3
DOIs	https://doi.org/10.1089/mdr.2009.0916
Publication status	Published - Sept 2009

Keywords

CONJUGATIVE TRANSPOSON TN916
ANTIBIOTIC-RESISTANCE
ENTEROCOCCUS-FAECALIS
ADULT PERIODONTITIS
RIBOSOMAL-RNA
HUMAN COLON
HUMAN GUT
BACTERIA
DIVERSITY
DETERMINANT

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1089/mdr.2009.0916

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@article{8533cde319c24684b801112b6d6831c7,

title = "Distribution of Tetracycline and Erythromycin Resistance Genes Among Human Oral and Fecal Metagenomic DNA",

abstract = "We have analyzed the total metagenomic DNA from both human oral and fecal samples derived from healthy volunteers from six European countries to determine the molecular basis for tetracycline and erythromycin resistance. We have determined that tet(M) and tet(W) are the most prevalent tetracycline resistance genes assayed for in the oral and fecal metagenomes, respectively. Additionally, tet(Q), tet(O), and tet(O/32/O) have been shown to be common. We have also shown that erm(B), erm(V), and erm(E) are common erythromycin resistance genes present in these environments. Further, we have demonstrated the ubiquitous presence of the Tn916 integrase in the oral metagenomes and the Tn4451 and Tn1549 integrase genes within the fecal metagenomes.",

keywords = "CONJUGATIVE TRANSPOSON TN916, ANTIBIOTIC-RESISTANCE, ENTEROCOCCUS-FAECALIS, ADULT PERIODONTITIS, RIBOSOMAL-RNA, HUMAN COLON, HUMAN GUT, BACTERIA, DIVERSITY, DETERMINANT",

author = "Seville, {Lorna A.} and Patterson, {Andrea J.} and Scott, {Karen Patricia} and Peter Mullany and Quail, {Michael A.} and Julian Parkhill and Derren Ready and Michael Wilson and David Spratt and Roberts, {Adam P.}",

year = "2009",

month = sep,

doi = "10.1089/mdr.2009.0916",

language = "English",

volume = "15",

pages = "159--166",

journal = "Microbial Drug Resistance",

issn = "1076-6294",

publisher = "Mary Ann Liebert Inc.",

number = "3",

}

TY - JOUR

T1 - Distribution of Tetracycline and Erythromycin Resistance Genes Among Human Oral and Fecal Metagenomic DNA

AU - Seville, Lorna A.

AU - Patterson, Andrea J.

AU - Scott, Karen Patricia

AU - Mullany, Peter

AU - Quail, Michael A.

AU - Parkhill, Julian

AU - Ready, Derren

AU - Wilson, Michael

AU - Spratt, David

AU - Roberts, Adam P.

PY - 2009/9

Y1 - 2009/9

N2 - We have analyzed the total metagenomic DNA from both human oral and fecal samples derived from healthy volunteers from six European countries to determine the molecular basis for tetracycline and erythromycin resistance. We have determined that tet(M) and tet(W) are the most prevalent tetracycline resistance genes assayed for in the oral and fecal metagenomes, respectively. Additionally, tet(Q), tet(O), and tet(O/32/O) have been shown to be common. We have also shown that erm(B), erm(V), and erm(E) are common erythromycin resistance genes present in these environments. Further, we have demonstrated the ubiquitous presence of the Tn916 integrase in the oral metagenomes and the Tn4451 and Tn1549 integrase genes within the fecal metagenomes.

AB - We have analyzed the total metagenomic DNA from both human oral and fecal samples derived from healthy volunteers from six European countries to determine the molecular basis for tetracycline and erythromycin resistance. We have determined that tet(M) and tet(W) are the most prevalent tetracycline resistance genes assayed for in the oral and fecal metagenomes, respectively. Additionally, tet(Q), tet(O), and tet(O/32/O) have been shown to be common. We have also shown that erm(B), erm(V), and erm(E) are common erythromycin resistance genes present in these environments. Further, we have demonstrated the ubiquitous presence of the Tn916 integrase in the oral metagenomes and the Tn4451 and Tn1549 integrase genes within the fecal metagenomes.

KW - CONJUGATIVE TRANSPOSON TN916

KW - ANTIBIOTIC-RESISTANCE

KW - ENTEROCOCCUS-FAECALIS

KW - ADULT PERIODONTITIS

KW - RIBOSOMAL-RNA

KW - HUMAN COLON

KW - HUMAN GUT

KW - BACTERIA

KW - DIVERSITY

KW - DETERMINANT

U2 - 10.1089/mdr.2009.0916

DO - 10.1089/mdr.2009.0916

M3 - Article

SN - 1076-6294

VL - 15

SP - 159

EP - 166

JO - Microbial Drug Resistance

JF - Microbial Drug Resistance

IS - 3

ER -

Distribution of Tetracycline and Erythromycin Resistance Genes Among Human Oral and Fecal Metagenomic DNA

Abstract

Keywords

UN SDGs

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