Distribution of Tetracycline and Erythromycin Resistance Genes Among Human Oral and Fecal Metagenomic DNA

Lorna A. Seville, Andrea J. Patterson, Karen Patricia Scott, Peter Mullany, Michael A. Quail, Julian Parkhill, Derren Ready, Michael Wilson, David Spratt, Adam P. Roberts

Research output: Contribution to journalArticle

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Abstract

We have analyzed the total metagenomic DNA from both human oral and fecal samples derived from healthy volunteers from six European countries to determine the molecular basis for tetracycline and erythromycin resistance. We have determined that tet(M) and tet(W) are the most prevalent tetracycline resistance genes assayed for in the oral and fecal metagenomes, respectively. Additionally, tet(Q), tet(O), and tet(O/32/O) have been shown to be common. We have also shown that erm(B), erm(V), and erm(E) are common erythromycin resistance genes present in these environments. Further, we have demonstrated the ubiquitous presence of the Tn916 integrase in the oral metagenomes and the Tn4451 and Tn1549 integrase genes within the fecal metagenomes.</p>
Original languageEnglish
Pages (from-to)159-166
Number of pages8
JournalMicrobial Drug Resistance
Volume15
Issue number3
DOIs
Publication statusPublished - Sep 2009

Keywords

  • CONJUGATIVE TRANSPOSON TN916
  • ANTIBIOTIC-RESISTANCE
  • ENTEROCOCCUS-FAECALIS
  • ADULT PERIODONTITIS
  • RIBOSOMAL-RNA
  • HUMAN COLON
  • HUMAN GUT
  • BACTERIA
  • DIVERSITY
  • DETERMINANT

Cite this

Seville, L. A., Patterson, A. J., Scott, K. P., Mullany, P., Quail, M. A., Parkhill, J., ... Roberts, A. P. (2009). Distribution of Tetracycline and Erythromycin Resistance Genes Among Human Oral and Fecal Metagenomic DNA. Microbial Drug Resistance, 15(3), 159-166. https://doi.org/10.1089/mdr.2009.0916

Distribution of Tetracycline and Erythromycin Resistance Genes Among Human Oral and Fecal Metagenomic DNA. / Seville, Lorna A.; Patterson, Andrea J.; Scott, Karen Patricia; Mullany, Peter; Quail, Michael A.; Parkhill, Julian; Ready, Derren; Wilson, Michael; Spratt, David; Roberts, Adam P.

In: Microbial Drug Resistance, Vol. 15, No. 3, 09.2009, p. 159-166.

Research output: Contribution to journalArticle

Seville, LA, Patterson, AJ, Scott, KP, Mullany, P, Quail, MA, Parkhill, J, Ready, D, Wilson, M, Spratt, D & Roberts, AP 2009, 'Distribution of Tetracycline and Erythromycin Resistance Genes Among Human Oral and Fecal Metagenomic DNA', Microbial Drug Resistance, vol. 15, no. 3, pp. 159-166. https://doi.org/10.1089/mdr.2009.0916
Seville, Lorna A. ; Patterson, Andrea J. ; Scott, Karen Patricia ; Mullany, Peter ; Quail, Michael A. ; Parkhill, Julian ; Ready, Derren ; Wilson, Michael ; Spratt, David ; Roberts, Adam P. / Distribution of Tetracycline and Erythromycin Resistance Genes Among Human Oral and Fecal Metagenomic DNA. In: Microbial Drug Resistance. 2009 ; Vol. 15, No. 3. pp. 159-166.
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abstract = "We have analyzed the total metagenomic DNA from both human oral and fecal samples derived from healthy volunteers from six European countries to determine the molecular basis for tetracycline and erythromycin resistance. We have determined that tet(M) and tet(W) are the most prevalent tetracycline resistance genes assayed for in the oral and fecal metagenomes, respectively. Additionally, tet(Q), tet(O), and tet(O/32/O) have been shown to be common. We have also shown that erm(B), erm(V), and erm(E) are common erythromycin resistance genes present in these environments. Further, we have demonstrated the ubiquitous presence of the Tn916 integrase in the oral metagenomes and the Tn4451 and Tn1549 integrase genes within the fecal metagenomes.",
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