Distribution of trace-elements in normal and diseased mouse ileum and kidney tissues

B J Kirby, H J McArdle, D M Danks, G J F Legge

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A proton microprobe has been utilised to determine the distribution and relative concentration of Cu, Fe and Zn in 10 day old normal and brindled mouse small intestine and kidney tissues. High Cu levels were measured in the brindled mutant ileum and kidney tissues confirming previous whole tissue results. In the ileum, peripheral concentrations of both Fe and Cu in normal and mutant villi tips were observed. Ratios of X-ray yields from outer villus tip to inner villus tip irradiated areas were taken and compared for mutant and normal mice after normalising to the bremsstrahlung yield in each case. The ratio of outer to inner Fe concentrations in the epithelium were shown to be higher than normal in the diseased tissue. In the kidney, the high Cu concentration in the mutant tissue was found to be localised to within certain regions in the proximal tubule in the nephron. Accurate determination of the Cu distribution in the small intestine and kidney tissues of the mutant mouse will provide further information on where the defective reabsorption of Cu is occurring, and may contribute to a better understanding of the homologous human condition.

Original languageEnglish
Pages (from-to)171-174
Number of pages4
JournalNuclear Instruments & Methods in Physics Research. Section B, Beam Interactions with Materials and Atoms
Volume54
Issue number1-3
DOIs
Publication statusPublished - Mar 1991

Keywords

  • kinky-hair syndrome
  • copper-metabolism
  • mutants
  • defect

Cite this

Distribution of trace-elements in normal and diseased mouse ileum and kidney tissues. / Kirby, B J ; McArdle, H J; Danks, D M ; Legge, G J F .

In: Nuclear Instruments & Methods in Physics Research. Section B, Beam Interactions with Materials and Atoms, Vol. 54, No. 1-3, 03.1991, p. 171-174.

Research output: Contribution to journalArticle

@article{3fbd1f78bc794c31a79e9ebc425f386f,
title = "Distribution of trace-elements in normal and diseased mouse ileum and kidney tissues",
abstract = "A proton microprobe has been utilised to determine the distribution and relative concentration of Cu, Fe and Zn in 10 day old normal and brindled mouse small intestine and kidney tissues. High Cu levels were measured in the brindled mutant ileum and kidney tissues confirming previous whole tissue results. In the ileum, peripheral concentrations of both Fe and Cu in normal and mutant villi tips were observed. Ratios of X-ray yields from outer villus tip to inner villus tip irradiated areas were taken and compared for mutant and normal mice after normalising to the bremsstrahlung yield in each case. The ratio of outer to inner Fe concentrations in the epithelium were shown to be higher than normal in the diseased tissue. In the kidney, the high Cu concentration in the mutant tissue was found to be localised to within certain regions in the proximal tubule in the nephron. Accurate determination of the Cu distribution in the small intestine and kidney tissues of the mutant mouse will provide further information on where the defective reabsorption of Cu is occurring, and may contribute to a better understanding of the homologous human condition.",
keywords = "kinky-hair syndrome, copper-metabolism, mutants, defect",
author = "Kirby, {B J} and McArdle, {H J} and Danks, {D M} and Legge, {G J F}",
year = "1991",
month = "3",
doi = "10.1016/0168-583X(91)95508-B",
language = "English",
volume = "54",
pages = "171--174",
journal = "Nuclear Instruments & Methods in Physics Research. Section B, Beam Interactions with Materials and Atoms",
issn = "0168-583X",
publisher = "Elsevier",
number = "1-3",

}

TY - JOUR

T1 - Distribution of trace-elements in normal and diseased mouse ileum and kidney tissues

AU - Kirby, B J

AU - McArdle, H J

AU - Danks, D M

AU - Legge, G J F

PY - 1991/3

Y1 - 1991/3

N2 - A proton microprobe has been utilised to determine the distribution and relative concentration of Cu, Fe and Zn in 10 day old normal and brindled mouse small intestine and kidney tissues. High Cu levels were measured in the brindled mutant ileum and kidney tissues confirming previous whole tissue results. In the ileum, peripheral concentrations of both Fe and Cu in normal and mutant villi tips were observed. Ratios of X-ray yields from outer villus tip to inner villus tip irradiated areas were taken and compared for mutant and normal mice after normalising to the bremsstrahlung yield in each case. The ratio of outer to inner Fe concentrations in the epithelium were shown to be higher than normal in the diseased tissue. In the kidney, the high Cu concentration in the mutant tissue was found to be localised to within certain regions in the proximal tubule in the nephron. Accurate determination of the Cu distribution in the small intestine and kidney tissues of the mutant mouse will provide further information on where the defective reabsorption of Cu is occurring, and may contribute to a better understanding of the homologous human condition.

AB - A proton microprobe has been utilised to determine the distribution and relative concentration of Cu, Fe and Zn in 10 day old normal and brindled mouse small intestine and kidney tissues. High Cu levels were measured in the brindled mutant ileum and kidney tissues confirming previous whole tissue results. In the ileum, peripheral concentrations of both Fe and Cu in normal and mutant villi tips were observed. Ratios of X-ray yields from outer villus tip to inner villus tip irradiated areas were taken and compared for mutant and normal mice after normalising to the bremsstrahlung yield in each case. The ratio of outer to inner Fe concentrations in the epithelium were shown to be higher than normal in the diseased tissue. In the kidney, the high Cu concentration in the mutant tissue was found to be localised to within certain regions in the proximal tubule in the nephron. Accurate determination of the Cu distribution in the small intestine and kidney tissues of the mutant mouse will provide further information on where the defective reabsorption of Cu is occurring, and may contribute to a better understanding of the homologous human condition.

KW - kinky-hair syndrome

KW - copper-metabolism

KW - mutants

KW - defect

U2 - 10.1016/0168-583X(91)95508-B

DO - 10.1016/0168-583X(91)95508-B

M3 - Article

VL - 54

SP - 171

EP - 174

JO - Nuclear Instruments & Methods in Physics Research. Section B, Beam Interactions with Materials and Atoms

JF - Nuclear Instruments & Methods in Physics Research. Section B, Beam Interactions with Materials and Atoms

SN - 0168-583X

IS - 1-3

ER -