A proton microprobe has been utilised to determine the distribution and relative concentration of Cu, Fe and Zn in 10 day old normal and brindled mouse small intestine and kidney tissues. High Cu levels were measured in the brindled mutant ileum and kidney tissues confirming previous whole tissue results. In the ileum, peripheral concentrations of both Fe and Cu in normal and mutant villi tips were observed. Ratios of X-ray yields from outer villus tip to inner villus tip irradiated areas were taken and compared for mutant and normal mice after normalising to the bremsstrahlung yield in each case. The ratio of outer to inner Fe concentrations in the epithelium were shown to be higher than normal in the diseased tissue. In the kidney, the high Cu concentration in the mutant tissue was found to be localised to within certain regions in the proximal tubule in the nephron. Accurate determination of the Cu distribution in the small intestine and kidney tissues of the mutant mouse will provide further information on where the defective reabsorption of Cu is occurring, and may contribute to a better understanding of the homologous human condition.
|Number of pages||4|
|Journal||Nuclear Instruments & Methods in Physics Research. Section B, Beam Interactions with Materials and Atoms|
|Publication status||Published - Mar 1991|
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