DISTRIBUTION OF XYLANASE GENES AND ENZYMES AMONG STRAINS OF PREVOTELLA (BACTEROIDES) RUMINICOLA FROM THE RUMEN

G AVGUSTIN, H J FLINT, T R WHITEHEAD

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The distribution of two xylanase genes was examined by Southern hybridization among 26 strains of the rumen anaerobic bacterium Prevotella (Bacteroides) ruminicola. Hybridization with a xylanase/endoglucanase gene from the type strain 23 was found in six strains while hybridization with a xylanase gene from strain D31d was found in 14 strains. Sequences related to both genes were present, on different restriction fragments, in six strains, whereas no hybridization to either gene was detected in five other strains capable of hydrolysing xylan, or in seven strains that showed little or no xylanase activity. Zymogram analyses of seven xylanolytic strains of P. ruminicola demonstrated interstrain variation in the apparent molecular masses of the major xylanases and carboxymethylcellulases that could be renatured following SDS polyacrylamide gel electrophoresis.

Original languageEnglish
Pages (from-to)137-143
Number of pages7
JournalFEMS Microbiology Letters
Volume99
Issue number2-3
Publication statusPublished - 1 Dec 1992

Keywords

  • XYLANASE
  • PREVOTELLA-RUMINICOLA
  • BACTEROIDES-RUMINICOLA
  • RUMEN BACTERIA
  • CARBOXYMETHYLCELLULASE
  • HYBRIDIZATION
  • BACTERIA
  • CLONING
  • ENUMERATION
  • EXPRESSION
  • CELLULASE

Cite this

DISTRIBUTION OF XYLANASE GENES AND ENZYMES AMONG STRAINS OF PREVOTELLA (BACTEROIDES) RUMINICOLA FROM THE RUMEN. / AVGUSTIN, G ; FLINT, H J ; WHITEHEAD, T R .

In: FEMS Microbiology Letters, Vol. 99, No. 2-3, 01.12.1992, p. 137-143.

Research output: Contribution to journalArticle

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title = "DISTRIBUTION OF XYLANASE GENES AND ENZYMES AMONG STRAINS OF PREVOTELLA (BACTEROIDES) RUMINICOLA FROM THE RUMEN",
abstract = "The distribution of two xylanase genes was examined by Southern hybridization among 26 strains of the rumen anaerobic bacterium Prevotella (Bacteroides) ruminicola. Hybridization with a xylanase/endoglucanase gene from the type strain 23 was found in six strains while hybridization with a xylanase gene from strain D31d was found in 14 strains. Sequences related to both genes were present, on different restriction fragments, in six strains, whereas no hybridization to either gene was detected in five other strains capable of hydrolysing xylan, or in seven strains that showed little or no xylanase activity. Zymogram analyses of seven xylanolytic strains of P. ruminicola demonstrated interstrain variation in the apparent molecular masses of the major xylanases and carboxymethylcellulases that could be renatured following SDS polyacrylamide gel electrophoresis.",
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AU - AVGUSTIN, G

AU - FLINT, H J

AU - WHITEHEAD, T R

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N2 - The distribution of two xylanase genes was examined by Southern hybridization among 26 strains of the rumen anaerobic bacterium Prevotella (Bacteroides) ruminicola. Hybridization with a xylanase/endoglucanase gene from the type strain 23 was found in six strains while hybridization with a xylanase gene from strain D31d was found in 14 strains. Sequences related to both genes were present, on different restriction fragments, in six strains, whereas no hybridization to either gene was detected in five other strains capable of hydrolysing xylan, or in seven strains that showed little or no xylanase activity. Zymogram analyses of seven xylanolytic strains of P. ruminicola demonstrated interstrain variation in the apparent molecular masses of the major xylanases and carboxymethylcellulases that could be renatured following SDS polyacrylamide gel electrophoresis.

AB - The distribution of two xylanase genes was examined by Southern hybridization among 26 strains of the rumen anaerobic bacterium Prevotella (Bacteroides) ruminicola. Hybridization with a xylanase/endoglucanase gene from the type strain 23 was found in six strains while hybridization with a xylanase gene from strain D31d was found in 14 strains. Sequences related to both genes were present, on different restriction fragments, in six strains, whereas no hybridization to either gene was detected in five other strains capable of hydrolysing xylan, or in seven strains that showed little or no xylanase activity. Zymogram analyses of seven xylanolytic strains of P. ruminicola demonstrated interstrain variation in the apparent molecular masses of the major xylanases and carboxymethylcellulases that could be renatured following SDS polyacrylamide gel electrophoresis.

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KW - PREVOTELLA-RUMINICOLA

KW - BACTEROIDES-RUMINICOLA

KW - RUMEN BACTERIA

KW - CARBOXYMETHYLCELLULASE

KW - HYBRIDIZATION

KW - BACTERIA

KW - CLONING

KW - ENUMERATION

KW - EXPRESSION

KW - CELLULASE

M3 - Article

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EP - 143

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

SN - 0378-1097

IS - 2-3

ER -