Divergent physiological characteristics and responses to endurance training among inbred mouse strains

A. Kilikevicius, T. Venckunas, R. Zelniene, A. M. Carroll, S. Lionikaite, A. Ratkevicius, A. Lionikas

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Both baseline values and adaptive changes in mice can vary depending on the genetic background. We aimed to assess variation in a battery of variables and their adaptations to endurance training in six inbred mouse strains. Males, n¿=¿184, from A/J, BALB/cByJ, C3H/HeJ, C57BL/6J, DBA/2J, and PWD/PhJ strains were assigned to a control or an endurance group (5 weeks swimming exercise). Enzyme activity, histology of soleus (SOL) muscle, swimming endurance, cardiac ventricular and hind limb muscle weight, and femur length were examined. Endurance capacity, morphological and histological variables, and enzyme activity substantially differed among strains. For example, SOL weight was twofold higher and cross-sectional area (CSA) of fibers was ~30% greater in C57BL/6J than in PWD/PhJ strain. The CSA of type 1 fibers were larger than type 2A in PWD/PhJ (P¿<¿0.01); however, the reverse was true in DBA/2J and BALB/cByJ strains (P¿<¿0.05). Swimming endurance in DBA/2J strain was ~9 times better than in BALB/cByJ. Endurance training increased the activity of citrate synthase in gastrocnemius across strains (P¿<¿0.01), however, changes in endurance were strain-specific; the C57BL/6J and DBA/2J strains improved substantially, whereas A/J and BALB/cByJ strains did not. In conclusion, genetic background is a potent determinant of the physiological characteristics and adaptations to training in mice.
Original languageEnglish
Pages (from-to)657-668
Number of pages11
JournalScandinavian Journal of Medicine & Science in Sports
Volume23
Issue number5
Early online date13 Mar 2012
DOIs
Publication statusPublished - Oct 2013

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Inbred Strains Mice
Physiological Adaptation
Weights and Measures
Citrate (si)-Synthase
Enzymes
Femur
Histology
Teaching
Skeletal Muscle
Extremities
Muscles
Genetic Background

Keywords

  • inbred strain
  • muscle fiber
  • heart
  • citrate synthase

Cite this

Divergent physiological characteristics and responses to endurance training among inbred mouse strains. / Kilikevicius, A.; Venckunas, T.; Zelniene, R.; Carroll, A. M.; Lionikaite, S.; Ratkevicius, A.; Lionikas, A.

In: Scandinavian Journal of Medicine & Science in Sports, Vol. 23, No. 5, 10.2013, p. 657-668.

Research output: Contribution to journalArticle

Kilikevicius, A. ; Venckunas, T. ; Zelniene, R. ; Carroll, A. M. ; Lionikaite, S. ; Ratkevicius, A. ; Lionikas, A. / Divergent physiological characteristics and responses to endurance training among inbred mouse strains. In: Scandinavian Journal of Medicine & Science in Sports. 2013 ; Vol. 23, No. 5. pp. 657-668.
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abstract = "Both baseline values and adaptive changes in mice can vary depending on the genetic background. We aimed to assess variation in a battery of variables and their adaptations to endurance training in six inbred mouse strains. Males, n¿=¿184, from A/J, BALB/cByJ, C3H/HeJ, C57BL/6J, DBA/2J, and PWD/PhJ strains were assigned to a control or an endurance group (5 weeks swimming exercise). Enzyme activity, histology of soleus (SOL) muscle, swimming endurance, cardiac ventricular and hind limb muscle weight, and femur length were examined. Endurance capacity, morphological and histological variables, and enzyme activity substantially differed among strains. For example, SOL weight was twofold higher and cross-sectional area (CSA) of fibers was ~30{\%} greater in C57BL/6J than in PWD/PhJ strain. The CSA of type 1 fibers were larger than type 2A in PWD/PhJ (P¿<¿0.01); however, the reverse was true in DBA/2J and BALB/cByJ strains (P¿<¿0.05). Swimming endurance in DBA/2J strain was ~9 times better than in BALB/cByJ. Endurance training increased the activity of citrate synthase in gastrocnemius across strains (P¿<¿0.01), however, changes in endurance were strain-specific; the C57BL/6J and DBA/2J strains improved substantially, whereas A/J and BALB/cByJ strains did not. In conclusion, genetic background is a potent determinant of the physiological characteristics and adaptations to training in mice.",
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