DLC1 is unlikely to be a primary target for deletions on chromosome arm 8p22 in head and neck squamous cell carcinoma

C Hewitt, P Wilson, E McGlinn, Gary Macfarlane, A Papageorgiou, R T M Woodwards, P Sloan, S M Gollin, I Paterson, K K Parkinson, A P Read, N Thakker

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Allelic imbalance on chromosome arm 8p is common in head and neck squamous cell carcinoma (HNSCC). DLC1, a tumour suppressor gene inactivated in liver carcinogenesis and encoding a Rho GTPase activating protein (RhoGAP) maps to one of the deleted regions (8p21.3-22). In order to determine whether inactivation of DLC1 is involved in HNSCC, we have screened turnout cell lines for DLC1 mutations and expression. Pathological mutations were not identified in any of the 17 cell lines tested. Seven polymorphisms were identified; 13 of the 17 of cell lines were homozygous for all seven polymorphisms compared to only 2 of 17 controls suggesting a loss of heterozygosity in a majority of the cell lines. DLC1 expression was observed in all 11 HNSCC cell lines tested, thus excluding the possibility of transcriptional silencing of DLC1 by promoter hypermethylation. Overall, our data suggest that hemizygous deletions of the DLC1 locus are frequent in HNSCCs but this gene is unlikely to be primary target for inactivation on this chromosomal arm. (C) 2004 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)207-213
Number of pages7
JournalCancer Letters
Volume209
Issue number2
DOIs
Publication statusPublished - 25 Jun 2004

Keywords

  • DLC1
  • tumour suppressor gene
  • head and neck squamous cell carcinoma
  • tumor-suppressor gene
  • allelic loss
  • hepatocellular carcinoma
  • cancer
  • lines
  • localization
  • progression
  • association
  • metastasis
  • GTpase
  • Tumour suppressor gene
  • Head and neck squamous cell carcinoma

Cite this

DLC1 is unlikely to be a primary target for deletions on chromosome arm 8p22 in head and neck squamous cell carcinoma. / Hewitt, C ; Wilson, P ; McGlinn, E ; Macfarlane, Gary; Papageorgiou, A ; Woodwards, R T M ; Sloan, P ; Gollin, S M ; Paterson, I ; Parkinson, K K ; Read, A P ; Thakker, N .

In: Cancer Letters, Vol. 209, No. 2, 25.06.2004, p. 207-213.

Research output: Contribution to journalArticle

Hewitt, C, Wilson, P, McGlinn, E, Macfarlane, G, Papageorgiou, A, Woodwards, RTM, Sloan, P, Gollin, SM, Paterson, I, Parkinson, KK, Read, AP & Thakker, N 2004, 'DLC1 is unlikely to be a primary target for deletions on chromosome arm 8p22 in head and neck squamous cell carcinoma' Cancer Letters, vol. 209, no. 2, pp. 207-213. https://doi.org/10.1016/j.canlet.2003.12.018
Hewitt, C ; Wilson, P ; McGlinn, E ; Macfarlane, Gary ; Papageorgiou, A ; Woodwards, R T M ; Sloan, P ; Gollin, S M ; Paterson, I ; Parkinson, K K ; Read, A P ; Thakker, N . / DLC1 is unlikely to be a primary target for deletions on chromosome arm 8p22 in head and neck squamous cell carcinoma. In: Cancer Letters. 2004 ; Vol. 209, No. 2. pp. 207-213.
@article{9cef29829a5f4c429689c29915fff463,
title = "DLC1 is unlikely to be a primary target for deletions on chromosome arm 8p22 in head and neck squamous cell carcinoma",
abstract = "Allelic imbalance on chromosome arm 8p is common in head and neck squamous cell carcinoma (HNSCC). DLC1, a tumour suppressor gene inactivated in liver carcinogenesis and encoding a Rho GTPase activating protein (RhoGAP) maps to one of the deleted regions (8p21.3-22). In order to determine whether inactivation of DLC1 is involved in HNSCC, we have screened turnout cell lines for DLC1 mutations and expression. Pathological mutations were not identified in any of the 17 cell lines tested. Seven polymorphisms were identified; 13 of the 17 of cell lines were homozygous for all seven polymorphisms compared to only 2 of 17 controls suggesting a loss of heterozygosity in a majority of the cell lines. DLC1 expression was observed in all 11 HNSCC cell lines tested, thus excluding the possibility of transcriptional silencing of DLC1 by promoter hypermethylation. Overall, our data suggest that hemizygous deletions of the DLC1 locus are frequent in HNSCCs but this gene is unlikely to be primary target for inactivation on this chromosomal arm. (C) 2004 Elsevier Ltd. All rights reserved.",
keywords = "DLC1, tumour suppressor gene, head and neck squamous cell carcinoma, tumor-suppressor gene, allelic loss, hepatocellular carcinoma, cancer, lines, localization, progression, association, metastasis, GTpase, Tumour suppressor gene, Head and neck squamous cell carcinoma",
author = "C Hewitt and P Wilson and E McGlinn and Gary Macfarlane and A Papageorgiou and Woodwards, {R T M} and P Sloan and Gollin, {S M} and I Paterson and Parkinson, {K K} and Read, {A P} and N Thakker",
year = "2004",
month = "6",
day = "25",
doi = "10.1016/j.canlet.2003.12.018",
language = "English",
volume = "209",
pages = "207--213",
journal = "Cancer Letters",
issn = "0304-3835",
publisher = "Elsevier Ireland Ltd",
number = "2",

}

TY - JOUR

T1 - DLC1 is unlikely to be a primary target for deletions on chromosome arm 8p22 in head and neck squamous cell carcinoma

AU - Hewitt, C

AU - Wilson, P

AU - McGlinn, E

AU - Macfarlane, Gary

AU - Papageorgiou, A

AU - Woodwards, R T M

AU - Sloan, P

AU - Gollin, S M

AU - Paterson, I

AU - Parkinson, K K

AU - Read, A P

AU - Thakker, N

PY - 2004/6/25

Y1 - 2004/6/25

N2 - Allelic imbalance on chromosome arm 8p is common in head and neck squamous cell carcinoma (HNSCC). DLC1, a tumour suppressor gene inactivated in liver carcinogenesis and encoding a Rho GTPase activating protein (RhoGAP) maps to one of the deleted regions (8p21.3-22). In order to determine whether inactivation of DLC1 is involved in HNSCC, we have screened turnout cell lines for DLC1 mutations and expression. Pathological mutations were not identified in any of the 17 cell lines tested. Seven polymorphisms were identified; 13 of the 17 of cell lines were homozygous for all seven polymorphisms compared to only 2 of 17 controls suggesting a loss of heterozygosity in a majority of the cell lines. DLC1 expression was observed in all 11 HNSCC cell lines tested, thus excluding the possibility of transcriptional silencing of DLC1 by promoter hypermethylation. Overall, our data suggest that hemizygous deletions of the DLC1 locus are frequent in HNSCCs but this gene is unlikely to be primary target for inactivation on this chromosomal arm. (C) 2004 Elsevier Ltd. All rights reserved.

AB - Allelic imbalance on chromosome arm 8p is common in head and neck squamous cell carcinoma (HNSCC). DLC1, a tumour suppressor gene inactivated in liver carcinogenesis and encoding a Rho GTPase activating protein (RhoGAP) maps to one of the deleted regions (8p21.3-22). In order to determine whether inactivation of DLC1 is involved in HNSCC, we have screened turnout cell lines for DLC1 mutations and expression. Pathological mutations were not identified in any of the 17 cell lines tested. Seven polymorphisms were identified; 13 of the 17 of cell lines were homozygous for all seven polymorphisms compared to only 2 of 17 controls suggesting a loss of heterozygosity in a majority of the cell lines. DLC1 expression was observed in all 11 HNSCC cell lines tested, thus excluding the possibility of transcriptional silencing of DLC1 by promoter hypermethylation. Overall, our data suggest that hemizygous deletions of the DLC1 locus are frequent in HNSCCs but this gene is unlikely to be primary target for inactivation on this chromosomal arm. (C) 2004 Elsevier Ltd. All rights reserved.

KW - DLC1

KW - tumour suppressor gene

KW - head and neck squamous cell carcinoma

KW - tumor-suppressor gene

KW - allelic loss

KW - hepatocellular carcinoma

KW - cancer

KW - lines

KW - localization

KW - progression

KW - association

KW - metastasis

KW - GTpase

KW - Tumour suppressor gene

KW - Head and neck squamous cell carcinoma

U2 - 10.1016/j.canlet.2003.12.018

DO - 10.1016/j.canlet.2003.12.018

M3 - Article

VL - 209

SP - 207

EP - 213

JO - Cancer Letters

JF - Cancer Letters

SN - 0304-3835

IS - 2

ER -