DLC1 is unlikely to be a primary target for deletions on chromosome arm 8p22 in head and neck squamous cell carcinoma

C Hewitt, P Wilson, E McGlinn, Gary Macfarlane, A Papageorgiou, R T M Woodwards, P Sloan, S M Gollin, I Paterson, K K Parkinson, A P Read, N Thakker

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Abstract

Allelic imbalance on chromosome arm 8p is common in head and neck squamous cell carcinoma (HNSCC). DLC1, a tumour suppressor gene inactivated in liver carcinogenesis and encoding a Rho GTPase activating protein (RhoGAP) maps to one of the deleted regions (8p21.3-22). In order to determine whether inactivation of DLC1 is involved in HNSCC, we have screened turnout cell lines for DLC1 mutations and expression. Pathological mutations were not identified in any of the 17 cell lines tested. Seven polymorphisms were identified; 13 of the 17 of cell lines were homozygous for all seven polymorphisms compared to only 2 of 17 controls suggesting a loss of heterozygosity in a majority of the cell lines. DLC1 expression was observed in all 11 HNSCC cell lines tested, thus excluding the possibility of transcriptional silencing of DLC1 by promoter hypermethylation. Overall, our data suggest that hemizygous deletions of the DLC1 locus are frequent in HNSCCs but this gene is unlikely to be primary target for inactivation on this chromosomal arm. (C) 2004 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)207-213
Number of pages7
JournalCancer Letters
Volume209
Issue number2
DOIs
Publication statusPublished - 25 Jun 2004

Keywords

  • DLC1
  • tumour suppressor gene
  • head and neck squamous cell carcinoma
  • tumor-suppressor gene
  • allelic loss
  • hepatocellular carcinoma
  • cancer
  • lines
  • localization
  • progression
  • association
  • metastasis
  • GTpase
  • Tumour suppressor gene
  • Head and neck squamous cell carcinoma

Cite this

Hewitt, C., Wilson, P., McGlinn, E., Macfarlane, G., Papageorgiou, A., Woodwards, R. T. M., Sloan, P., Gollin, S. M., Paterson, I., Parkinson, K. K., Read, A. P., & Thakker, N. (2004). DLC1 is unlikely to be a primary target for deletions on chromosome arm 8p22 in head and neck squamous cell carcinoma. Cancer Letters, 209(2), 207-213. https://doi.org/10.1016/j.canlet.2003.12.018