DNA from historical and trophy samples provides insights into white shark population origins and genetic diversity

Chrysoula Gubili, Cory E C Robinson, Geremy Cliff, Sabine P. Wintner, Eleonora de Sabata, Sabina De Innocentiis, Simonepietro Canese, David W. Sims, Andrew P. Martin, Leslie R. Noble, Catherine S. Jones*

*Corresponding author for this work

Research output: Contribution to journalArticle

5 Citations (Scopus)
5 Downloads (Pure)

Abstract

Characterizing genetic variation by retrospective genotyping of trophy or historical artifacts from endangered species is an important conservation tool. Loss of genetic diversity in top predators such as the white shark Carcharodon carcharias remains an issue, exacerbated in this species by declining, sometimes isolated philopatric populations. We successfully sequenced mitochondrial DNA (mtDNA) D-loop from osteodentine of contemporary South African white shark teeth (from 3 jaws), and from 34 to 129 yr old dried cartilage and skin samples from 1 Pacific Ocean and 5 Mediterranean sharks. Osteodentine-derived sequences from South African fish matched those derived from an individual's finclips, but were generally of poorer quality than those from skin and cartilage of historical samples. Three haplotypes were identified from historical Mediterranean samples (n = 5); 2 individuals had unique sequences and 3 shared the contemporary Mediterranean haplotype. Placement of previously undescribed mtDNA haplotypes from historical material within both the Mediterranean and Pacific clades fits with the accepted intraspecific phylogeny derived from contemporary material, verifying our approaches. The utility of our methodology is in its provision of additional genetic resources from osteodentine (for species lacking tooth pulp) and cartilage of rare and endangered species held in often uncurated, contemporary and historical dry collections. Such material can usefully supplement estimates of connectivity, population history, and stock viability. We confirm the depauperate haplotype diversity of historical Mediterranean sharks, consistent with founding by a small number of Pacific colonizers. The consequent lack of diversity suggests serious challenges for the maintenance of this top predator and the Mediterranean ecosystem.

Original languageEnglish
Pages (from-to)233-241
Number of pages9
JournalEndangered Species Research
Volume27
Issue number3
DOIs
Publication statusPublished - 2015

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shark
DNA
endangered species
mitochondrial DNA
tooth
skin
predator
genetic resource
isolated population
rare species
artifact
genetic variation
connectivity
viability
phylogeny
methodology
genetic diversity
ecosystem
ocean
fish

Keywords

  • Carcharodon carcharias
  • Cartilage
  • Genotyping
  • Mediterranean
  • Mitochondrial DNA
  • Museum specimens
  • Osteodentine
  • Teeth
  • White shark

ASJC Scopus subject areas

  • Nature and Landscape Conservation
  • Ecology

Cite this

DNA from historical and trophy samples provides insights into white shark population origins and genetic diversity. / Gubili, Chrysoula; Robinson, Cory E C; Cliff, Geremy; Wintner, Sabine P.; de Sabata, Eleonora; De Innocentiis, Sabina; Canese, Simonepietro; Sims, David W.; Martin, Andrew P.; Noble, Leslie R.; Jones, Catherine S.

In: Endangered Species Research, Vol. 27, No. 3, 2015, p. 233-241.

Research output: Contribution to journalArticle

Gubili, C, Robinson, CEC, Cliff, G, Wintner, SP, de Sabata, E, De Innocentiis, S, Canese, S, Sims, DW, Martin, AP, Noble, LR & Jones, CS 2015, 'DNA from historical and trophy samples provides insights into white shark population origins and genetic diversity', Endangered Species Research, vol. 27, no. 3, pp. 233-241. https://doi.org/10.3354/esr00665
Gubili, Chrysoula ; Robinson, Cory E C ; Cliff, Geremy ; Wintner, Sabine P. ; de Sabata, Eleonora ; De Innocentiis, Sabina ; Canese, Simonepietro ; Sims, David W. ; Martin, Andrew P. ; Noble, Leslie R. ; Jones, Catherine S. / DNA from historical and trophy samples provides insights into white shark population origins and genetic diversity. In: Endangered Species Research. 2015 ; Vol. 27, No. 3. pp. 233-241.
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