DNA vaccination against viral haemorrhagic septicaemia (VHS) in rainbow trout: size, dose, route of injection and duration of protection - early protection correlates with Mx expression.

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Abstract

Rainbow trout of different sizes (10 and 100 g) were injected intramuscularly (i.m.) or intraperitoneally (i.p.) with different doses (range 10ng-10mug) of a viral haemorrhagic septicaemia (VHS)-DNA vaccine (pcDNA3vhsG). As controls, fish were injected with the pcDNA3 plasmid alone, or with inactivated VHS virus. Fish were challenged at different times post-vaccination (p.v.) to assess protection. At certain times p.v., serum samples were analysed for neutralising antibody and liver tissue was analysed for Mx mRNA expression.

A DNA dose of 0.5 mug injected by the i.m. route induced protection in fish of all sizes in challenges performed either I or 4 weeks p.v. This dose also conferred effective protection up to 9 months p.v. in fish >100 g. With lower doses of DNA (0.1 and 0.01 mug) and challenge at 4 weeks p.v., 10 g fish were partially protected but protection was not observed in 100 g fish. Vaccination by the i.p. route induced no or lower levels of protection compared with the i.m. route.

Fish vaccinated with 0.5 mug DNA i.m. had no detectable serum neutralising antibody (NAb) at 4 weeks p.v. (with the exception of a single 10 g fish) but antibody was detected at 8 weeks and 6 months p.v. but not at 9 months p.v. However, cohorts of these fish showed effective protection at all timepoints. Lack of detectable levels of NAb (at 9 weeks p.v.) despite partial protection in challenge at 4 weeks p.v. was also observed with 0.01 mug doses of DNA i.m. NAb was detected in sera of fish at 8 weeks after vaccination with 0.1 mug i.m. but not in fish vaccinated with doses of 0.01-0.5 mug i.p. Early protection (1 week p.v.) correlated with elevated Mx gene expression. (C) 2003 Elsevier Science Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)39-50
Number of pages11
JournalFish & Shellfish Immunology
Volume15
DOIs
Publication statusPublished - 2003

Keywords

  • viral haemorrhagic septicaemia
  • DNA-vaccination
  • trout
  • protection
  • neutralising antibody
  • Mx
  • HEMATOPOIETIC NECROSIS VIRUS
  • HEMORRHAGIC SEPTICEMIA VIRUS
  • ONCORHYNCHUS-MYKISS
  • VACCINES
  • IMMUNIZATION
  • IMMUNITY
  • PROTEIN
  • FISH
  • RHABDOVIRUSES
  • RESISTANCE

Cite this

@article{6bcab586dbdf49a8a13c0c431d64100d,
title = "DNA vaccination against viral haemorrhagic septicaemia (VHS) in rainbow trout: size, dose, route of injection and duration of protection - early protection correlates with Mx expression.",
abstract = "Rainbow trout of different sizes (10 and 100 g) were injected intramuscularly (i.m.) or intraperitoneally (i.p.) with different doses (range 10ng-10mug) of a viral haemorrhagic septicaemia (VHS)-DNA vaccine (pcDNA3vhsG). As controls, fish were injected with the pcDNA3 plasmid alone, or with inactivated VHS virus. Fish were challenged at different times post-vaccination (p.v.) to assess protection. At certain times p.v., serum samples were analysed for neutralising antibody and liver tissue was analysed for Mx mRNA expression.A DNA dose of 0.5 mug injected by the i.m. route induced protection in fish of all sizes in challenges performed either I or 4 weeks p.v. This dose also conferred effective protection up to 9 months p.v. in fish >100 g. With lower doses of DNA (0.1 and 0.01 mug) and challenge at 4 weeks p.v., 10 g fish were partially protected but protection was not observed in 100 g fish. Vaccination by the i.p. route induced no or lower levels of protection compared with the i.m. route.Fish vaccinated with 0.5 mug DNA i.m. had no detectable serum neutralising antibody (NAb) at 4 weeks p.v. (with the exception of a single 10 g fish) but antibody was detected at 8 weeks and 6 months p.v. but not at 9 months p.v. However, cohorts of these fish showed effective protection at all timepoints. Lack of detectable levels of NAb (at 9 weeks p.v.) despite partial protection in challenge at 4 weeks p.v. was also observed with 0.01 mug doses of DNA i.m. NAb was detected in sera of fish at 8 weeks after vaccination with 0.1 mug i.m. but not in fish vaccinated with doses of 0.01-0.5 mug i.p. Early protection (1 week p.v.) correlated with elevated Mx gene expression. (C) 2003 Elsevier Science Ltd. All rights reserved.",
keywords = "viral haemorrhagic septicaemia, DNA-vaccination, trout, protection, neutralising antibody, Mx, HEMATOPOIETIC NECROSIS VIRUS, HEMORRHAGIC SEPTICEMIA VIRUS, ONCORHYNCHUS-MYKISS, VACCINES, IMMUNIZATION, IMMUNITY, PROTEIN, FISH, RHABDOVIRUSES, RESISTANCE",
author = "Secombes, {Christopher John}",
year = "2003",
doi = "10.1016/S1050-4648(02)00137-7",
language = "English",
volume = "15",
pages = "39--50",
journal = "Fish & Shellfish Immunology",
issn = "1050-4648",
publisher = "ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD",

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TY - JOUR

T1 - DNA vaccination against viral haemorrhagic septicaemia (VHS) in rainbow trout: size, dose, route of injection and duration of protection - early protection correlates with Mx expression.

AU - Secombes, Christopher John

PY - 2003

Y1 - 2003

N2 - Rainbow trout of different sizes (10 and 100 g) were injected intramuscularly (i.m.) or intraperitoneally (i.p.) with different doses (range 10ng-10mug) of a viral haemorrhagic septicaemia (VHS)-DNA vaccine (pcDNA3vhsG). As controls, fish were injected with the pcDNA3 plasmid alone, or with inactivated VHS virus. Fish were challenged at different times post-vaccination (p.v.) to assess protection. At certain times p.v., serum samples were analysed for neutralising antibody and liver tissue was analysed for Mx mRNA expression.A DNA dose of 0.5 mug injected by the i.m. route induced protection in fish of all sizes in challenges performed either I or 4 weeks p.v. This dose also conferred effective protection up to 9 months p.v. in fish >100 g. With lower doses of DNA (0.1 and 0.01 mug) and challenge at 4 weeks p.v., 10 g fish were partially protected but protection was not observed in 100 g fish. Vaccination by the i.p. route induced no or lower levels of protection compared with the i.m. route.Fish vaccinated with 0.5 mug DNA i.m. had no detectable serum neutralising antibody (NAb) at 4 weeks p.v. (with the exception of a single 10 g fish) but antibody was detected at 8 weeks and 6 months p.v. but not at 9 months p.v. However, cohorts of these fish showed effective protection at all timepoints. Lack of detectable levels of NAb (at 9 weeks p.v.) despite partial protection in challenge at 4 weeks p.v. was also observed with 0.01 mug doses of DNA i.m. NAb was detected in sera of fish at 8 weeks after vaccination with 0.1 mug i.m. but not in fish vaccinated with doses of 0.01-0.5 mug i.p. Early protection (1 week p.v.) correlated with elevated Mx gene expression. (C) 2003 Elsevier Science Ltd. All rights reserved.

AB - Rainbow trout of different sizes (10 and 100 g) were injected intramuscularly (i.m.) or intraperitoneally (i.p.) with different doses (range 10ng-10mug) of a viral haemorrhagic septicaemia (VHS)-DNA vaccine (pcDNA3vhsG). As controls, fish were injected with the pcDNA3 plasmid alone, or with inactivated VHS virus. Fish were challenged at different times post-vaccination (p.v.) to assess protection. At certain times p.v., serum samples were analysed for neutralising antibody and liver tissue was analysed for Mx mRNA expression.A DNA dose of 0.5 mug injected by the i.m. route induced protection in fish of all sizes in challenges performed either I or 4 weeks p.v. This dose also conferred effective protection up to 9 months p.v. in fish >100 g. With lower doses of DNA (0.1 and 0.01 mug) and challenge at 4 weeks p.v., 10 g fish were partially protected but protection was not observed in 100 g fish. Vaccination by the i.p. route induced no or lower levels of protection compared with the i.m. route.Fish vaccinated with 0.5 mug DNA i.m. had no detectable serum neutralising antibody (NAb) at 4 weeks p.v. (with the exception of a single 10 g fish) but antibody was detected at 8 weeks and 6 months p.v. but not at 9 months p.v. However, cohorts of these fish showed effective protection at all timepoints. Lack of detectable levels of NAb (at 9 weeks p.v.) despite partial protection in challenge at 4 weeks p.v. was also observed with 0.01 mug doses of DNA i.m. NAb was detected in sera of fish at 8 weeks after vaccination with 0.1 mug i.m. but not in fish vaccinated with doses of 0.01-0.5 mug i.p. Early protection (1 week p.v.) correlated with elevated Mx gene expression. (C) 2003 Elsevier Science Ltd. All rights reserved.

KW - viral haemorrhagic septicaemia

KW - DNA-vaccination

KW - trout

KW - protection

KW - neutralising antibody

KW - Mx

KW - HEMATOPOIETIC NECROSIS VIRUS

KW - HEMORRHAGIC SEPTICEMIA VIRUS

KW - ONCORHYNCHUS-MYKISS

KW - VACCINES

KW - IMMUNIZATION

KW - IMMUNITY

KW - PROTEIN

KW - FISH

KW - RHABDOVIRUSES

KW - RESISTANCE

U2 - 10.1016/S1050-4648(02)00137-7

DO - 10.1016/S1050-4648(02)00137-7

M3 - Article

VL - 15

SP - 39

EP - 50

JO - Fish & Shellfish Immunology

JF - Fish & Shellfish Immunology

SN - 1050-4648

ER -