Does carbon flow from mycorrhizal fungi stimulate bacterial antibiotic production?

Eleni Siasou, Dominic Benjamin Standing, Ken Killham, David Johnson

Research output: Contribution to conferencePoster

Abstract

The presence of mycorrhizal-root associations markedly alters the quality of carbon flow from roots. We investigated how antibiotic production of the model Pseudomonas fluorescens strains were modulated by qualitative changes to C-flow. Specifically, we quantified the production of the antibiotic 2,4-diacetylphloroglucinol (DAPG), a known suppressant of root fungal pathogens. In the first experiment, wheat plants were grown for five weeks in sand inside split pots so that half of their root systems were colonised by arbuscular mycorrhizal fungi and half remained uncolonised. In the second experiment, wheat plants were grown without mycorrhizal fungi, with mycorrhizal fungi and with the root pathogen Gaeumannomyces graminis var. tritici (Ggt) and with both mycorrhizal fungi and Ggt. Roots were harvested and homogenate was used to challenge cultures of P. fluorescens strains. Each homogenate was balanced for molar C. To increase sample throughput and analytical efficiency, we used a 96-well plate Porvair Sciences Ltd Microlute® system containing a C-18 Solid Phase Extraction matrix. The analyte of interest (DAPG) in each cell in the growth plate was retained on the C-18 matrix until elution by methanol for HPLC analysis. We discuss how the presence of the arbuscular mycorrhizal fungi and Ggt affects the antibiotic production by root associated bacteria.
Original languageEnglish
Publication statusPublished - 2008
EventEurosoil - Vienna, Austria
Duration: 25 Aug 200829 Aug 2008

Conference

ConferenceEurosoil
CountryAustria
CityVienna
Period25/08/0829/08/08

Fingerprint

mycorrhizal fungi
antibiotics
Gaeumannomyces graminis var. tritici
carbon
Pseudomonas fluorescens
wheat
growth plate
pathogens
solid phase extraction
root systems
cell growth
methanol
sand
bacteria
sampling

Cite this

Siasou, E., Standing, D. B., Killham, K., & Johnson, D. (2008). Does carbon flow from mycorrhizal fungi stimulate bacterial antibiotic production?. Poster session presented at Eurosoil, Vienna, Austria.

Does carbon flow from mycorrhizal fungi stimulate bacterial antibiotic production? / Siasou, Eleni; Standing, Dominic Benjamin; Killham, Ken; Johnson, David.

2008. Poster session presented at Eurosoil, Vienna, Austria.

Research output: Contribution to conferencePoster

Siasou, E, Standing, DB, Killham, K & Johnson, D 2008, 'Does carbon flow from mycorrhizal fungi stimulate bacterial antibiotic production?' Eurosoil, Vienna, Austria, 25/08/08 - 29/08/08, .
Siasou E, Standing DB, Killham K, Johnson D. Does carbon flow from mycorrhizal fungi stimulate bacterial antibiotic production?. 2008. Poster session presented at Eurosoil, Vienna, Austria.
Siasou, Eleni ; Standing, Dominic Benjamin ; Killham, Ken ; Johnson, David. / Does carbon flow from mycorrhizal fungi stimulate bacterial antibiotic production?. Poster session presented at Eurosoil, Vienna, Austria.
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N2 - The presence of mycorrhizal-root associations markedly alters the quality of carbon flow from roots. We investigated how antibiotic production of the model Pseudomonas fluorescens strains were modulated by qualitative changes to C-flow. Specifically, we quantified the production of the antibiotic 2,4-diacetylphloroglucinol (DAPG), a known suppressant of root fungal pathogens. In the first experiment, wheat plants were grown for five weeks in sand inside split pots so that half of their root systems were colonised by arbuscular mycorrhizal fungi and half remained uncolonised. In the second experiment, wheat plants were grown without mycorrhizal fungi, with mycorrhizal fungi and with the root pathogen Gaeumannomyces graminis var. tritici (Ggt) and with both mycorrhizal fungi and Ggt. Roots were harvested and homogenate was used to challenge cultures of P. fluorescens strains. Each homogenate was balanced for molar C. To increase sample throughput and analytical efficiency, we used a 96-well plate Porvair Sciences Ltd Microlute® system containing a C-18 Solid Phase Extraction matrix. The analyte of interest (DAPG) in each cell in the growth plate was retained on the C-18 matrix until elution by methanol for HPLC analysis. We discuss how the presence of the arbuscular mycorrhizal fungi and Ggt affects the antibiotic production by root associated bacteria.

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