Does exercise-induced hypoxemia modify lactate influx into erythrocytes and hemorheological parameters in athletes?

P. Connes, D. Bouix, G. Py, C. Caillaud, Pascale Kippelen, J. F. Brun, A. Varray, C. Prefaut, J. Mercier

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    Abstract

    This study investigated 1) red blood cells (RBC) rigidity and 2) lactate influxes into RBCs in endurance-trained athletes with and without exercise-induced hypoxemia (EIH). Nine EIH and six non-EIH subjects performed a submaximal steady-state exercise on a cycloergometer at 60% of maximal aerobic power for 10 min, followed by 15 min at 85% of maximal aerobic power. At rest and at the end of exercise, arterialized blood was sampled for analysis of arterialized pressure in oxygen, and venous blood was drawn for analysis of plasma lactate concentrations and hemorheological parameters. Lactate influxes into RBCs were measured at three labeled [U-C-14] lactate concentrations (1.6, 8.1, and 41 mM) on venous blood sampled at rest. The EIH subjects had higher maximal oxygen uptake than non-EIH ( P < 0.05). Total lactate influx was significantly higher in RBCs from EIH compared with non-EIH subjects at 8.1 mM ( 1,498.1 +/- 87.8 vs. 1,035.9 +/- 114.8 nmol &BULL; ml(-1) &BULL; min(-1); P < 0.05) and 41 mM ( 2,562.0 +/- 145.0 vs. 1,618.1 +/- 149.4 nmol . ml(-1) . min(-1); P < 0.01). Monocarboxylate transporter-1-mediated lactate influx was also higher in EIH at 8.1 mM ( P < 0.05) and 41 mM ( P < 0.01). The drop in arterial oxygen partial pressure was negatively correlated with total lactate influx measured at 8.1 mM ( r = - 0.82, P < 0.05) and 41 mM ( r = - 0.84, P < 0.05) in the two groups together. Plasma lactate concentrations and hemorheological data were similar in the two groups at rest and at the end of exercise. The results showed higher monocarboxylate transporter-1-mediated lactate influx in the EIH subjects and suggested that EIH could modify lactate influx into erythrocyte. However, higher lactate influx in EIH subjects was not accompanied by an increase in RBC rigidity.

    Original languageEnglish
    Pages (from-to)1053-1058
    Number of pages5
    JournalJournal of Applied Physiology
    Volume97
    Issue number3
    DOIs
    Publication statusPublished - Sep 2004

    Keywords

    • monocarboxylate transporter
    • endurance
    • lactate metabolism
    • hypoxemia
    • hemorheology
    • FATTY-ACID DIET
    • INDUCED ARTERIAL HYPOXEMIA
    • RED-BLOOD-CELLS
    • EAR OXIMETER
    • VISCOSITY
    • MUSCLE
    • DESATURATION
    • TRANSPORT
    • HYPOXIA
    • VOLUME

    Cite this

    Does exercise-induced hypoxemia modify lactate influx into erythrocytes and hemorheological parameters in athletes? / Connes, P.; Bouix, D.; Py, G.; Caillaud, C.; Kippelen, Pascale; Brun, J. F.; Varray, A.; Prefaut, C.; Mercier, J.

    In: Journal of Applied Physiology, Vol. 97, No. 3, 09.2004, p. 1053-1058.

    Research output: Contribution to journalArticle

    Connes, P, Bouix, D, Py, G, Caillaud, C, Kippelen, P, Brun, JF, Varray, A, Prefaut, C & Mercier, J 2004, 'Does exercise-induced hypoxemia modify lactate influx into erythrocytes and hemorheological parameters in athletes?', Journal of Applied Physiology, vol. 97, no. 3, pp. 1053-1058. https://doi.org/10.1152/japplphysiol.00993.2003
    Connes, P. ; Bouix, D. ; Py, G. ; Caillaud, C. ; Kippelen, Pascale ; Brun, J. F. ; Varray, A. ; Prefaut, C. ; Mercier, J. / Does exercise-induced hypoxemia modify lactate influx into erythrocytes and hemorheological parameters in athletes?. In: Journal of Applied Physiology. 2004 ; Vol. 97, No. 3. pp. 1053-1058.
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    abstract = "This study investigated 1) red blood cells (RBC) rigidity and 2) lactate influxes into RBCs in endurance-trained athletes with and without exercise-induced hypoxemia (EIH). Nine EIH and six non-EIH subjects performed a submaximal steady-state exercise on a cycloergometer at 60{\%} of maximal aerobic power for 10 min, followed by 15 min at 85{\%} of maximal aerobic power. At rest and at the end of exercise, arterialized blood was sampled for analysis of arterialized pressure in oxygen, and venous blood was drawn for analysis of plasma lactate concentrations and hemorheological parameters. Lactate influxes into RBCs were measured at three labeled [U-C-14] lactate concentrations (1.6, 8.1, and 41 mM) on venous blood sampled at rest. The EIH subjects had higher maximal oxygen uptake than non-EIH ( P < 0.05). Total lactate influx was significantly higher in RBCs from EIH compared with non-EIH subjects at 8.1 mM ( 1,498.1 +/- 87.8 vs. 1,035.9 +/- 114.8 nmol &BULL; ml(-1) &BULL; min(-1); P < 0.05) and 41 mM ( 2,562.0 +/- 145.0 vs. 1,618.1 +/- 149.4 nmol . ml(-1) . min(-1); P < 0.01). Monocarboxylate transporter-1-mediated lactate influx was also higher in EIH at 8.1 mM ( P < 0.05) and 41 mM ( P < 0.01). The drop in arterial oxygen partial pressure was negatively correlated with total lactate influx measured at 8.1 mM ( r = - 0.82, P < 0.05) and 41 mM ( r = - 0.84, P < 0.05) in the two groups together. Plasma lactate concentrations and hemorheological data were similar in the two groups at rest and at the end of exercise. The results showed higher monocarboxylate transporter-1-mediated lactate influx in the EIH subjects and suggested that EIH could modify lactate influx into erythrocyte. However, higher lactate influx in EIH subjects was not accompanied by an increase in RBC rigidity.",
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    T1 - Does exercise-induced hypoxemia modify lactate influx into erythrocytes and hemorheological parameters in athletes?

    AU - Connes, P.

    AU - Bouix, D.

    AU - Py, G.

    AU - Caillaud, C.

    AU - Kippelen, Pascale

    AU - Brun, J. F.

    AU - Varray, A.

    AU - Prefaut, C.

    AU - Mercier, J.

    PY - 2004/9

    Y1 - 2004/9

    N2 - This study investigated 1) red blood cells (RBC) rigidity and 2) lactate influxes into RBCs in endurance-trained athletes with and without exercise-induced hypoxemia (EIH). Nine EIH and six non-EIH subjects performed a submaximal steady-state exercise on a cycloergometer at 60% of maximal aerobic power for 10 min, followed by 15 min at 85% of maximal aerobic power. At rest and at the end of exercise, arterialized blood was sampled for analysis of arterialized pressure in oxygen, and venous blood was drawn for analysis of plasma lactate concentrations and hemorheological parameters. Lactate influxes into RBCs were measured at three labeled [U-C-14] lactate concentrations (1.6, 8.1, and 41 mM) on venous blood sampled at rest. The EIH subjects had higher maximal oxygen uptake than non-EIH ( P < 0.05). Total lactate influx was significantly higher in RBCs from EIH compared with non-EIH subjects at 8.1 mM ( 1,498.1 +/- 87.8 vs. 1,035.9 +/- 114.8 nmol &BULL; ml(-1) &BULL; min(-1); P < 0.05) and 41 mM ( 2,562.0 +/- 145.0 vs. 1,618.1 +/- 149.4 nmol . ml(-1) . min(-1); P < 0.01). Monocarboxylate transporter-1-mediated lactate influx was also higher in EIH at 8.1 mM ( P < 0.05) and 41 mM ( P < 0.01). The drop in arterial oxygen partial pressure was negatively correlated with total lactate influx measured at 8.1 mM ( r = - 0.82, P < 0.05) and 41 mM ( r = - 0.84, P < 0.05) in the two groups together. Plasma lactate concentrations and hemorheological data were similar in the two groups at rest and at the end of exercise. The results showed higher monocarboxylate transporter-1-mediated lactate influx in the EIH subjects and suggested that EIH could modify lactate influx into erythrocyte. However, higher lactate influx in EIH subjects was not accompanied by an increase in RBC rigidity.

    AB - This study investigated 1) red blood cells (RBC) rigidity and 2) lactate influxes into RBCs in endurance-trained athletes with and without exercise-induced hypoxemia (EIH). Nine EIH and six non-EIH subjects performed a submaximal steady-state exercise on a cycloergometer at 60% of maximal aerobic power for 10 min, followed by 15 min at 85% of maximal aerobic power. At rest and at the end of exercise, arterialized blood was sampled for analysis of arterialized pressure in oxygen, and venous blood was drawn for analysis of plasma lactate concentrations and hemorheological parameters. Lactate influxes into RBCs were measured at three labeled [U-C-14] lactate concentrations (1.6, 8.1, and 41 mM) on venous blood sampled at rest. The EIH subjects had higher maximal oxygen uptake than non-EIH ( P < 0.05). Total lactate influx was significantly higher in RBCs from EIH compared with non-EIH subjects at 8.1 mM ( 1,498.1 +/- 87.8 vs. 1,035.9 +/- 114.8 nmol &BULL; ml(-1) &BULL; min(-1); P < 0.05) and 41 mM ( 2,562.0 +/- 145.0 vs. 1,618.1 +/- 149.4 nmol . ml(-1) . min(-1); P < 0.01). Monocarboxylate transporter-1-mediated lactate influx was also higher in EIH at 8.1 mM ( P < 0.05) and 41 mM ( P < 0.01). The drop in arterial oxygen partial pressure was negatively correlated with total lactate influx measured at 8.1 mM ( r = - 0.82, P < 0.05) and 41 mM ( r = - 0.84, P < 0.05) in the two groups together. Plasma lactate concentrations and hemorheological data were similar in the two groups at rest and at the end of exercise. The results showed higher monocarboxylate transporter-1-mediated lactate influx in the EIH subjects and suggested that EIH could modify lactate influx into erythrocyte. However, higher lactate influx in EIH subjects was not accompanied by an increase in RBC rigidity.

    KW - monocarboxylate transporter

    KW - endurance

    KW - lactate metabolism

    KW - hypoxemia

    KW - hemorheology

    KW - FATTY-ACID DIET

    KW - INDUCED ARTERIAL HYPOXEMIA

    KW - RED-BLOOD-CELLS

    KW - EAR OXIMETER

    KW - VISCOSITY

    KW - MUSCLE

    KW - DESATURATION

    KW - TRANSPORT

    KW - HYPOXIA

    KW - VOLUME

    U2 - 10.1152/japplphysiol.00993.2003

    DO - 10.1152/japplphysiol.00993.2003

    M3 - Article

    VL - 97

    SP - 1053

    EP - 1058

    JO - Journal of Applied Physiology

    JF - Journal of Applied Physiology

    SN - 8750-7587

    IS - 3

    ER -