Dominant and diet-responsive groups of bacteria within the human colonic microbiota

Alan W Walker, Jennifer Ince, Sylvia H Duncan, Lucy Webster, Grietje Holtrop, Xiaolei Ze, David Brown, Mark D. Stares, Paul Scott, Aurore Bergerat, Petra Louis, Freda McIntosh, Alexandra M Johnstone, Gerald E Lobley, Julian Parkhill, Harry J Flint

Research output: Contribution to journalArticle

709 Citations (Scopus)

Abstract

The populations of dominant species within the human colonic microbiota can potentially be modified by dietary intake with consequences for health. Here we examined the influence of precisely controlled diets in 14 overweight men. Volunteers were provided successively with a control diet, diets high in resistant starch (RS) or non-starch polysaccharides (NSPs) and a reduced carbohydrate weight loss (WL) diet, over 10 weeks. Analysis of 16S rRNA sequences in stool samples of six volunteers detected 320 phylotypes (defined at > 98% identity) of which 26, including 19 cultured species, each accounted for > 1% of sequences. Although samples clustered more strongly by individual than by diet, time courses obtained by targeted qPCR revealed that 'blooms' in specific bacterial groups occurred rapidly after a dietary change. These were rapidly reversed by the subsequent diet. Relatives of Ruminococcus bromii (R-ruminococci) increased in most volunteers on the RS diet, accounting for a mean of 17% of total bacteria compared with 3.8% on the NSP diet, whereas the uncultured Oscillibacter group increased on the RS and WL diets. Relatives of Eubacterium rectale increased on RS (to mean 10.1%) but decreased, along with Collinsella aerofaciens, on WL. Inter-individual variation was marked, however, with > 60% of RS remaining unfermented in two volunteers on the RS diet, compared to < 4% in the other 12 volunteers; these two individuals also showed low numbers of R-ruminococci (< 1%). Dietary non-digestible carbohydrate can produce marked changes in the gut microbiota, but these depend on the initial composition of an individual's gut microbiota. The ISME Journal (2011) 5, 220-230; doi:10.1038/ISMEJ.2010.118; published online 5 August 2010

Original languageEnglish
Pages (from-to)220-230
Number of pages11
JournalThe ISME Journal
Volume5
Issue number2
Early online date5 Aug 2010
DOIs
Publication statusPublished - Feb 2011

Keywords

  • archaea
  • bacteria
  • biodiversity
  • colon
  • cross-over studies
  • diet
  • diet, reducing
  • dietary carbohydrates
  • dietary proteins
  • feces
  • humans
  • male
  • metagenome
  • RNA, ribosomal, 16S
  • starch

Cite this

Dominant and diet-responsive groups of bacteria within the human colonic microbiota. / Walker, Alan W; Ince, Jennifer; Duncan, Sylvia H; Webster, Lucy; Holtrop, Grietje; Ze, Xiaolei; Brown, David; Stares, Mark D.; Scott, Paul; Bergerat, Aurore; Louis, Petra; McIntosh, Freda; Johnstone, Alexandra M; Lobley, Gerald E; Parkhill, Julian; Flint, Harry J.

In: The ISME Journal, Vol. 5, No. 2, 02.2011, p. 220-230.

Research output: Contribution to journalArticle

Walker, AW, Ince, J, Duncan, SH, Webster, L, Holtrop, G, Ze, X, Brown, D, Stares, MD, Scott, P, Bergerat, A, Louis, P, McIntosh, F, Johnstone, AM, Lobley, GE, Parkhill, J & Flint, HJ 2011, 'Dominant and diet-responsive groups of bacteria within the human colonic microbiota', The ISME Journal, vol. 5, no. 2, pp. 220-230. https://doi.org/10.1038/ismej.2010.118
Walker, Alan W ; Ince, Jennifer ; Duncan, Sylvia H ; Webster, Lucy ; Holtrop, Grietje ; Ze, Xiaolei ; Brown, David ; Stares, Mark D. ; Scott, Paul ; Bergerat, Aurore ; Louis, Petra ; McIntosh, Freda ; Johnstone, Alexandra M ; Lobley, Gerald E ; Parkhill, Julian ; Flint, Harry J. / Dominant and diet-responsive groups of bacteria within the human colonic microbiota. In: The ISME Journal. 2011 ; Vol. 5, No. 2. pp. 220-230.
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