Dot-blotting--a novel screening assay for antibodies in hybridoma cultures

Jeremy Sternberg, Peter Jeppesen

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

A novel solid phase radioimmunoassay using nitrocellulose filter, is described. The method is of particular value in screening hybridoma cultures for monoclonal antibodies against chromosomal protein. Also it permits detection of antibodies directed against epitopes stable to SDS denaturation
Original languageEnglish
Pages (from-to)39-43
Number of pages5
JournalJournal of Immunological Methods
Volume64
Issue number1-2
DOIs
Publication statusPublished - Nov 1983

Keywords

  • radioimmunoassay
  • monoclonal antibody
  • chromosome protein

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