Downregulation of Caspase 8 in a group of Iranian breast cancer patients – A pilot study

Masoumeh Aghababazadeh, Najmeh Dorraki, Fahimeh Afzal Javan, Asieh Sadat Fattahi, Masoumeh Gharib, Alireza Pasdar*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

Purpose It is now well known that evading apoptosis, as a cancer hallmark, can lead to tumour initiation, progression and metastasis. As a result of genome wide association studies, an initiator protease in this pathway, caspase 8 (CASP8), has been found to be an important gene regarding breast cancer susceptibility. The alterations of the expression of this gene have been reported in breast cancer cell lines. Given that in previous studies expression analysis of this gene had only been done in breast cancer cell lines, in this study we aimed to evaluate the expression of this gene in breast cancer tissues versus adjacent normal tissues, using real-time quantitative method. Methods Caspase 8 mRNA expression was quantified using comparative RT-qPCR in 27 fresh frozen breast tumours and 27 adjacent normal tissues. Moreover, relationship between the expression changes of CASP8 in tumour tissue and various clinical and pathological features were evaluated in an Iranian population. Results The present study showed that expression of CASP8 was significantly reduced in tumour tissues compared to neighbouring normal tissues (p =.004). CASP8 expression was significantly correlated with the status of hormone receptors (ER and PR). Conclusion To the best of our knowledge, this study is the first report on reduced expression of CASP8 in breast cancer versus adjacent normal tissues. Our data support previous results obtained from cell lines and therefore highlights the seminal role of the induction of CASP8 expression, as a novel therapeutic approach, in order to sensitize tumour cells to apoptotic stimuli.

Original languageEnglish
Pages (from-to)191-195
Number of pages5
JournalJournal of the Egyptian National Cancer Institute
Volume29
Issue number4
Early online date7 Dec 2017
DOIs
Publication statusPublished - Dec 2017

Keywords

  • Apoptosis
  • Breast cancer
  • Caspase 8

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