TY - JOUR
T1 - Downregulation of Caspase 8 in a group of Iranian breast cancer patients – A pilot study
AU - Aghababazadeh, Masoumeh
AU - Dorraki, Najmeh
AU - Javan, Fahimeh Afzal
AU - Fattahi, Asieh Sadat
AU - Gharib, Masoumeh
AU - Pasdar, Alireza
N1 - Acknowledgments
Author contributions: MA performed related laboratory work, helped with sample collection, analysed the data and drafted the manuscript. ND and FAJ helped with sample collection and laboratory work. ASF and MG confirmed the diagnosis and provided the appropriate specimens. AP conceived and designed the study, supervised the project and edited the manuscript.
The authors would like to thank the research council of Mashhad University of Medical Sciences, Mashhad, Iran for the financial support (Grant Number: 940936).
PY - 2017/12
Y1 - 2017/12
N2 - Purpose It is now well known that evading apoptosis, as a cancer hallmark, can lead to tumour initiation, progression and metastasis. As a result of genome wide association studies, an initiator protease in this pathway, caspase 8 (CASP8), has been found to be an important gene regarding breast cancer susceptibility. The alterations of the expression of this gene have been reported in breast cancer cell lines. Given that in previous studies expression analysis of this gene had only been done in breast cancer cell lines, in this study we aimed to evaluate the expression of this gene in breast cancer tissues versus adjacent normal tissues, using real-time quantitative method. Methods Caspase 8 mRNA expression was quantified using comparative RT-qPCR in 27 fresh frozen breast tumours and 27 adjacent normal tissues. Moreover, relationship between the expression changes of CASP8 in tumour tissue and various clinical and pathological features were evaluated in an Iranian population. Results The present study showed that expression of CASP8 was significantly reduced in tumour tissues compared to neighbouring normal tissues (p =.004). CASP8 expression was significantly correlated with the status of hormone receptors (ER and PR). Conclusion To the best of our knowledge, this study is the first report on reduced expression of CASP8 in breast cancer versus adjacent normal tissues. Our data support previous results obtained from cell lines and therefore highlights the seminal role of the induction of CASP8 expression, as a novel therapeutic approach, in order to sensitize tumour cells to apoptotic stimuli.
AB - Purpose It is now well known that evading apoptosis, as a cancer hallmark, can lead to tumour initiation, progression and metastasis. As a result of genome wide association studies, an initiator protease in this pathway, caspase 8 (CASP8), has been found to be an important gene regarding breast cancer susceptibility. The alterations of the expression of this gene have been reported in breast cancer cell lines. Given that in previous studies expression analysis of this gene had only been done in breast cancer cell lines, in this study we aimed to evaluate the expression of this gene in breast cancer tissues versus adjacent normal tissues, using real-time quantitative method. Methods Caspase 8 mRNA expression was quantified using comparative RT-qPCR in 27 fresh frozen breast tumours and 27 adjacent normal tissues. Moreover, relationship between the expression changes of CASP8 in tumour tissue and various clinical and pathological features were evaluated in an Iranian population. Results The present study showed that expression of CASP8 was significantly reduced in tumour tissues compared to neighbouring normal tissues (p =.004). CASP8 expression was significantly correlated with the status of hormone receptors (ER and PR). Conclusion To the best of our knowledge, this study is the first report on reduced expression of CASP8 in breast cancer versus adjacent normal tissues. Our data support previous results obtained from cell lines and therefore highlights the seminal role of the induction of CASP8 expression, as a novel therapeutic approach, in order to sensitize tumour cells to apoptotic stimuli.
KW - Apoptosis
KW - Breast cancer
KW - Caspase 8
UR - http://www.scopus.com/inward/record.url?scp=85038087950&partnerID=8YFLogxK
U2 - 10.1016/j.jnci.2017.10.001
DO - 10.1016/j.jnci.2017.10.001
M3 - Article
C2 - 29233452
AN - SCOPUS:85038087950
VL - 29
SP - 191
EP - 195
JO - Journal of the Egyptian National Cancer Institute
JF - Journal of the Egyptian National Cancer Institute
SN - 1110-0362
IS - 4
ER -
