DSCAM and DSCAML1 function in self-avoidance in multiple cell types in the developing mouse retina

Peter G Fuerst, Freyja Bruce, Miao Tian, Wei Wei, Justin Elstrott, Marla B Feller, Lynda Erskine, Joshua H Singer, Robert W Burgess

Research output: Contribution to journalArticle

148 Citations (Scopus)

Abstract

DSCAM and DSCAM-LIKE1 (DSCAML1) serve diverse neurodevelopmental functions, including axon guidance, synaptic adhesion, and self-avoidance, depending on the species, cell type, and gene family member studied. We examined the function of DSCAM and DSCAML1 in the developing mouse retina. In addition to a subset of amacrine cells, Dscam was expressed in most retinal ganglion cells (RGCs). RGCs had fasciculated dendrites and clumped cell bodies in Dscam(-/-) mice, suggesting a role in self-avoidance. Dscaml1 was expressed in the rod circuit, and mice lacking Dscaml1 had fasciculated rod bipolar cell dendrites and clumped AII amacrine cell bodies, also indicating a role in self-avoidance. Neurons in Dscam or Dscaml1 mutant retinas stratified their processes appropriately in synaptic laminae in the inner plexiform layer, and functional synapses formed in the rod circuit in mice lacking Dscaml1. Therefore, DSCAM and DSCAML1 function similarly in self-avoidance, and are not essential for synaptic specificity in the mouse retina.
Original languageEnglish
Pages (from-to)484-497
Number of pages14
JournalNeuron
Volume64
Issue number4
DOIs
Publication statusPublished - 25 Nov 2009

Fingerprint

Retina
Amacrine Cells
Retinal Ganglion Cells
Dendrites
Synapses
Neurons
Genes
Cell Body

Keywords

  • animals
  • avoidance learning
  • cell adhesion molecules
  • mice
  • mice, inbred C57BL
  • mice, knockout
  • mice, transgenic
  • neurites
  • retina
  • synapses

Cite this

Fuerst, P. G., Bruce, F., Tian, M., Wei, W., Elstrott, J., Feller, M. B., ... Burgess, R. W. (2009). DSCAM and DSCAML1 function in self-avoidance in multiple cell types in the developing mouse retina. Neuron, 64(4), 484-497. https://doi.org/10.1016/j.neuron.2009.09.027

DSCAM and DSCAML1 function in self-avoidance in multiple cell types in the developing mouse retina. / Fuerst, Peter G; Bruce, Freyja; Tian, Miao; Wei, Wei; Elstrott, Justin; Feller, Marla B; Erskine, Lynda; Singer, Joshua H; Burgess, Robert W.

In: Neuron, Vol. 64, No. 4, 25.11.2009, p. 484-497.

Research output: Contribution to journalArticle

Fuerst, PG, Bruce, F, Tian, M, Wei, W, Elstrott, J, Feller, MB, Erskine, L, Singer, JH & Burgess, RW 2009, 'DSCAM and DSCAML1 function in self-avoidance in multiple cell types in the developing mouse retina', Neuron, vol. 64, no. 4, pp. 484-497. https://doi.org/10.1016/j.neuron.2009.09.027
Fuerst, Peter G ; Bruce, Freyja ; Tian, Miao ; Wei, Wei ; Elstrott, Justin ; Feller, Marla B ; Erskine, Lynda ; Singer, Joshua H ; Burgess, Robert W. / DSCAM and DSCAML1 function in self-avoidance in multiple cell types in the developing mouse retina. In: Neuron. 2009 ; Vol. 64, No. 4. pp. 484-497.
@article{a52a5358bea74d1192870f238dcaef6f,
title = "DSCAM and DSCAML1 function in self-avoidance in multiple cell types in the developing mouse retina",
abstract = "DSCAM and DSCAM-LIKE1 (DSCAML1) serve diverse neurodevelopmental functions, including axon guidance, synaptic adhesion, and self-avoidance, depending on the species, cell type, and gene family member studied. We examined the function of DSCAM and DSCAML1 in the developing mouse retina. In addition to a subset of amacrine cells, Dscam was expressed in most retinal ganglion cells (RGCs). RGCs had fasciculated dendrites and clumped cell bodies in Dscam(-/-) mice, suggesting a role in self-avoidance. Dscaml1 was expressed in the rod circuit, and mice lacking Dscaml1 had fasciculated rod bipolar cell dendrites and clumped AII amacrine cell bodies, also indicating a role in self-avoidance. Neurons in Dscam or Dscaml1 mutant retinas stratified their processes appropriately in synaptic laminae in the inner plexiform layer, and functional synapses formed in the rod circuit in mice lacking Dscaml1. Therefore, DSCAM and DSCAML1 function similarly in self-avoidance, and are not essential for synaptic specificity in the mouse retina.",
keywords = "animals, avoidance learning, cell adhesion molecules, mice, mice, inbred C57BL, mice, knockout, mice, transgenic, neurites, retina, synapses",
author = "Fuerst, {Peter G} and Freyja Bruce and Miao Tian and Wei Wei and Justin Elstrott and Feller, {Marla B} and Lynda Erskine and Singer, {Joshua H} and Burgess, {Robert W}",
note = "Previewed in: Neuron 64, 441-443.",
year = "2009",
month = "11",
day = "25",
doi = "10.1016/j.neuron.2009.09.027",
language = "English",
volume = "64",
pages = "484--497",
journal = "Neuron",
issn = "0896-6273",
publisher = "Cell Press",
number = "4",

}

TY - JOUR

T1 - DSCAM and DSCAML1 function in self-avoidance in multiple cell types in the developing mouse retina

AU - Fuerst, Peter G

AU - Bruce, Freyja

AU - Tian, Miao

AU - Wei, Wei

AU - Elstrott, Justin

AU - Feller, Marla B

AU - Erskine, Lynda

AU - Singer, Joshua H

AU - Burgess, Robert W

N1 - Previewed in: Neuron 64, 441-443.

PY - 2009/11/25

Y1 - 2009/11/25

N2 - DSCAM and DSCAM-LIKE1 (DSCAML1) serve diverse neurodevelopmental functions, including axon guidance, synaptic adhesion, and self-avoidance, depending on the species, cell type, and gene family member studied. We examined the function of DSCAM and DSCAML1 in the developing mouse retina. In addition to a subset of amacrine cells, Dscam was expressed in most retinal ganglion cells (RGCs). RGCs had fasciculated dendrites and clumped cell bodies in Dscam(-/-) mice, suggesting a role in self-avoidance. Dscaml1 was expressed in the rod circuit, and mice lacking Dscaml1 had fasciculated rod bipolar cell dendrites and clumped AII amacrine cell bodies, also indicating a role in self-avoidance. Neurons in Dscam or Dscaml1 mutant retinas stratified their processes appropriately in synaptic laminae in the inner plexiform layer, and functional synapses formed in the rod circuit in mice lacking Dscaml1. Therefore, DSCAM and DSCAML1 function similarly in self-avoidance, and are not essential for synaptic specificity in the mouse retina.

AB - DSCAM and DSCAM-LIKE1 (DSCAML1) serve diverse neurodevelopmental functions, including axon guidance, synaptic adhesion, and self-avoidance, depending on the species, cell type, and gene family member studied. We examined the function of DSCAM and DSCAML1 in the developing mouse retina. In addition to a subset of amacrine cells, Dscam was expressed in most retinal ganglion cells (RGCs). RGCs had fasciculated dendrites and clumped cell bodies in Dscam(-/-) mice, suggesting a role in self-avoidance. Dscaml1 was expressed in the rod circuit, and mice lacking Dscaml1 had fasciculated rod bipolar cell dendrites and clumped AII amacrine cell bodies, also indicating a role in self-avoidance. Neurons in Dscam or Dscaml1 mutant retinas stratified their processes appropriately in synaptic laminae in the inner plexiform layer, and functional synapses formed in the rod circuit in mice lacking Dscaml1. Therefore, DSCAM and DSCAML1 function similarly in self-avoidance, and are not essential for synaptic specificity in the mouse retina.

KW - animals

KW - avoidance learning

KW - cell adhesion molecules

KW - mice

KW - mice, inbred C57BL

KW - mice, knockout

KW - mice, transgenic

KW - neurites

KW - retina

KW - synapses

U2 - 10.1016/j.neuron.2009.09.027

DO - 10.1016/j.neuron.2009.09.027

M3 - Article

VL - 64

SP - 484

EP - 497

JO - Neuron

JF - Neuron

SN - 0896-6273

IS - 4

ER -