Dynamics of DNA replication in yeast

R. Retkute, C. Nieduszynski, Alessandro de Moura

Research output: Contribution to journalArticle

26 Citations (Scopus)
4 Downloads (Pure)

Abstract

We present a mathematical model for the spatial dynamics of DNA replication. Using this model we determine the probability distribution for the time at which each chromosomal position is replicated. From this we show, contrary to previous reports, that mean replication time curves cannot be used to directly determine origin parameters. We demonstrate that the stochastic nature of replication dynamics leaves a clear signature in experimentally measured population average data, and we show that the width of the activation time probability distribution can be inferred from this data. Our results compare favorably with experimental measurements in Saccharomyces cerevisae.

Original languageEnglish
Article number068103
Number of pages4
JournalPhysical Review Letters
Volume107
Issue number6
Early online date4 Aug 2011
DOIs
Publication statusPublished - 5 Aug 2011

Fingerprint

yeast
deoxyribonucleic acid
saccharomyces
leaves
mathematical models
signatures
activation
curves

Cite this

Dynamics of DNA replication in yeast. / Retkute, R.; Nieduszynski, C.; de Moura, Alessandro .

In: Physical Review Letters, Vol. 107, No. 6, 068103, 05.08.2011.

Research output: Contribution to journalArticle

Retkute, R. ; Nieduszynski, C. ; de Moura, Alessandro . / Dynamics of DNA replication in yeast. In: Physical Review Letters. 2011 ; Vol. 107, No. 6.
@article{cee03bf8183f447c8078ef678485d3b2,
title = "Dynamics of DNA replication in yeast",
abstract = "We present a mathematical model for the spatial dynamics of DNA replication. Using this model we determine the probability distribution for the time at which each chromosomal position is replicated. From this we show, contrary to previous reports, that mean replication time curves cannot be used to directly determine origin parameters. We demonstrate that the stochastic nature of replication dynamics leaves a clear signature in experimentally measured population average data, and we show that the width of the activation time probability distribution can be inferred from this data. Our results compare favorably with experimental measurements in Saccharomyces cerevisae.",
author = "R. Retkute and C. Nieduszynski and {de Moura}, Alessandro",
year = "2011",
month = "8",
day = "5",
doi = "10.1103/PhysRevLett.107.068103",
language = "English",
volume = "107",
journal = "Physical Review Letters",
issn = "0031-9007",
publisher = "American Physical Society",
number = "6",

}

TY - JOUR

T1 - Dynamics of DNA replication in yeast

AU - Retkute, R.

AU - Nieduszynski, C.

AU - de Moura, Alessandro

PY - 2011/8/5

Y1 - 2011/8/5

N2 - We present a mathematical model for the spatial dynamics of DNA replication. Using this model we determine the probability distribution for the time at which each chromosomal position is replicated. From this we show, contrary to previous reports, that mean replication time curves cannot be used to directly determine origin parameters. We demonstrate that the stochastic nature of replication dynamics leaves a clear signature in experimentally measured population average data, and we show that the width of the activation time probability distribution can be inferred from this data. Our results compare favorably with experimental measurements in Saccharomyces cerevisae.

AB - We present a mathematical model for the spatial dynamics of DNA replication. Using this model we determine the probability distribution for the time at which each chromosomal position is replicated. From this we show, contrary to previous reports, that mean replication time curves cannot be used to directly determine origin parameters. We demonstrate that the stochastic nature of replication dynamics leaves a clear signature in experimentally measured population average data, and we show that the width of the activation time probability distribution can be inferred from this data. Our results compare favorably with experimental measurements in Saccharomyces cerevisae.

U2 - 10.1103/PhysRevLett.107.068103

DO - 10.1103/PhysRevLett.107.068103

M3 - Article

VL - 107

JO - Physical Review Letters

JF - Physical Review Letters

SN - 0031-9007

IS - 6

M1 - 068103

ER -