Early initiation of a replication origin tethered at the nuclear periphery

Hani Ebrahimi, E. Douglas Robertson, Angela Taddei, Susan M. Gasser, Anne Dunlop Donaldson, Shin-Ichiro Hiraga

Research output: Contribution to journalArticle

23 Citations (Scopus)
3 Downloads (Pure)

Abstract

Peripheral nuclear localization of chromosomal loci correlates with late replication in yeast and metazoan cells. To test whether peripheral positioning can impose late replication, we examined whether artificial tethering of an early-initiating replication origin to the nuclear periphery delays its replication in budding yeast. We tested the effects of three different peripheral tethering constructs on the time of replication of the early replication origin ARS607. Using the dense-isotope transfer method to assess replication time, we found that ARS607 still replicates early when tethered to the nuclear periphery using the Yif1 protein or a fragment of Sir4, whereas tethering using a Yku80 construct produces only a very slight replication delay. Single-cell microscopic analysis revealed no correlation between peripheral positioning of ARS607 in individual cells and delayed replication. Overall, our results demonstrate that a replication origin can initiate replication early in S phase, even if artificially relocated to the nuclear periphery.
Original languageEnglish
Pages (from-to)1015-1019
JournalJournal of Cell Science
Volume123
Early online date2 Mar 2010
DOIs
Publication statusPublished - 23 Mar 2010

Fingerprint

Replication Origin
Single-Cell Analysis
Saccharomycetales
S Phase
Isotopes
Yeasts
Proteins

Cite this

Early initiation of a replication origin tethered at the nuclear periphery. / Ebrahimi, Hani; Robertson, E. Douglas; Taddei, Angela; Gasser, Susan M.; Donaldson, Anne Dunlop; Hiraga, Shin-Ichiro.

In: Journal of Cell Science, Vol. 123, 23.03.2010, p. 1015-1019.

Research output: Contribution to journalArticle

Ebrahimi, Hani ; Robertson, E. Douglas ; Taddei, Angela ; Gasser, Susan M. ; Donaldson, Anne Dunlop ; Hiraga, Shin-Ichiro. / Early initiation of a replication origin tethered at the nuclear periphery. In: Journal of Cell Science. 2010 ; Vol. 123. pp. 1015-1019.
@article{6d4a397d7cff4a75bc1e11f2a42d51c1,
title = "Early initiation of a replication origin tethered at the nuclear periphery",
abstract = "Peripheral nuclear localization of chromosomal loci correlates with late replication in yeast and metazoan cells. To test whether peripheral positioning can impose late replication, we examined whether artificial tethering of an early-initiating replication origin to the nuclear periphery delays its replication in budding yeast. We tested the effects of three different peripheral tethering constructs on the time of replication of the early replication origin ARS607. Using the dense-isotope transfer method to assess replication time, we found that ARS607 still replicates early when tethered to the nuclear periphery using the Yif1 protein or a fragment of Sir4, whereas tethering using a Yku80 construct produces only a very slight replication delay. Single-cell microscopic analysis revealed no correlation between peripheral positioning of ARS607 in individual cells and delayed replication. Overall, our results demonstrate that a replication origin can initiate replication early in S phase, even if artificially relocated to the nuclear periphery.",
author = "Hani Ebrahimi and Robertson, {E. Douglas} and Angela Taddei and Gasser, {Susan M.} and Donaldson, {Anne Dunlop} and Shin-Ichiro Hiraga",
year = "2010",
month = "3",
day = "23",
doi = "10.1242/jcs.060392",
language = "English",
volume = "123",
pages = "1015--1019",
journal = "Journal of Cell Science",
issn = "0021-9533",
publisher = "Company of Biologists Ltd",

}

TY - JOUR

T1 - Early initiation of a replication origin tethered at the nuclear periphery

AU - Ebrahimi, Hani

AU - Robertson, E. Douglas

AU - Taddei, Angela

AU - Gasser, Susan M.

AU - Donaldson, Anne Dunlop

AU - Hiraga, Shin-Ichiro

PY - 2010/3/23

Y1 - 2010/3/23

N2 - Peripheral nuclear localization of chromosomal loci correlates with late replication in yeast and metazoan cells. To test whether peripheral positioning can impose late replication, we examined whether artificial tethering of an early-initiating replication origin to the nuclear periphery delays its replication in budding yeast. We tested the effects of three different peripheral tethering constructs on the time of replication of the early replication origin ARS607. Using the dense-isotope transfer method to assess replication time, we found that ARS607 still replicates early when tethered to the nuclear periphery using the Yif1 protein or a fragment of Sir4, whereas tethering using a Yku80 construct produces only a very slight replication delay. Single-cell microscopic analysis revealed no correlation between peripheral positioning of ARS607 in individual cells and delayed replication. Overall, our results demonstrate that a replication origin can initiate replication early in S phase, even if artificially relocated to the nuclear periphery.

AB - Peripheral nuclear localization of chromosomal loci correlates with late replication in yeast and metazoan cells. To test whether peripheral positioning can impose late replication, we examined whether artificial tethering of an early-initiating replication origin to the nuclear periphery delays its replication in budding yeast. We tested the effects of three different peripheral tethering constructs on the time of replication of the early replication origin ARS607. Using the dense-isotope transfer method to assess replication time, we found that ARS607 still replicates early when tethered to the nuclear periphery using the Yif1 protein or a fragment of Sir4, whereas tethering using a Yku80 construct produces only a very slight replication delay. Single-cell microscopic analysis revealed no correlation between peripheral positioning of ARS607 in individual cells and delayed replication. Overall, our results demonstrate that a replication origin can initiate replication early in S phase, even if artificially relocated to the nuclear periphery.

U2 - 10.1242/jcs.060392

DO - 10.1242/jcs.060392

M3 - Article

VL - 123

SP - 1015

EP - 1019

JO - Journal of Cell Science

JF - Journal of Cell Science

SN - 0021-9533

ER -