Ectomycorrhizal fungal exoenzyme activity differs on spruce seedlings planted in forests versus clearcuts

Jennifer K. M. Walker*, Valerie Ward, Melanie D. Jones

*Corresponding author for this work

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Ectomycorrhizal (ECM) fungal community composition is altered by forest harvesting, but it is not clear if this shift in structure influences ECM fungal physiological function at the community level. In this study, we characterized activities of extracellular enzymes in the ectomycorrhizospheres of Picea engelmannii seedlings grown in forest and clearcut plots. These exoenzymes are critical for the breakdown of large organic molecules, from which nutrients are subsequently absorbed and translocated by ECM fungi to host plants. We found that ectomycorrhizae on seedlings planted in forests had different exoenzyme activity profiles than those on seedlings planted in clearcuts. Specifically, the activities of glucuronidase, laccase, and acid phosphatase were higher on forest seedlings (P

Original languageEnglish
Pages (from-to)497-508
Number of pages12
JournalTrees-Structure and function
Volume30
Issue number2
Early online date8 Jul 2015
DOIs
Publication statusPublished - Apr 2016
Event6th International Symposium on Physiological Processes in Roots of Woody Plants - Nagoya, Japan
Duration: 1 Sep 2014 → …

Keywords

  • Exoenzyme assay
  • Ectomycorrhizal physiology
  • Functional complementarity and redundancy
  • Picea engelmannii
  • Bioassay seedling
  • Clearcut harvesting
  • Community structure
  • British-Columbia
  • Enzyme-activity
  • Soil
  • Diversity
  • Microorganisms
  • Phosphatase
  • Stands

Cite this

Ectomycorrhizal fungal exoenzyme activity differs on spruce seedlings planted in forests versus clearcuts. / Walker, Jennifer K. M.; Ward, Valerie; Jones, Melanie D.

In: Trees-Structure and function, Vol. 30, No. 2, 04.2016, p. 497-508.

Research output: Contribution to journalArticle

Walker, Jennifer K. M. ; Ward, Valerie ; Jones, Melanie D. / Ectomycorrhizal fungal exoenzyme activity differs on spruce seedlings planted in forests versus clearcuts. In: Trees-Structure and function. 2016 ; Vol. 30, No. 2. pp. 497-508.
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abstract = "Ectomycorrhizal (ECM) fungal community composition is altered by forest harvesting, but it is not clear if this shift in structure influences ECM fungal physiological function at the community level. In this study, we characterized activities of extracellular enzymes in the ectomycorrhizospheres of Picea engelmannii seedlings grown in forest and clearcut plots. These exoenzymes are critical for the breakdown of large organic molecules, from which nutrients are subsequently absorbed and translocated by ECM fungi to host plants. We found that ectomycorrhizae on seedlings planted in forests had different exoenzyme activity profiles than those on seedlings planted in clearcuts. Specifically, the activities of glucuronidase, laccase, and acid phosphatase were higher on forest seedlings (P",
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note = "This research was supported by a Discovery Grant from the Natural Sciences and Engineering Research Council of Canada and by the Forest Investment Account of the Forest Science Program of the British Columbia (BC) Ministry of Forests, Lands and Natural Resource Operations (MFLNRO) awarded to MJ. JW acknowledges support from the University of British Columbia, Okanagan Campus and the Province of British Columbia for scholarships. We thank Alan Vyse for access to information about the Sicamous Creek field site, MaryAnn Olson, Fawn Ross, and Brendan Twieg for help in the field, and Natasha Lukey and Ayla Fortin for help in the field and lab. Finally, we are immensely grateful to the BC MFLNRO for establishment and long-term maintenance of the Sicamous Creek Silvicultural System Research Project (http://www.for.gov.bc.ca/rsi/research/sicamous/index.htm).",
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