Ectomycorrhizal fungal exoenzyme activity differs on spruce seedlings planted in forests versus clearcuts

Jennifer K. M. Walker; Valerie Ward; Melanie D. Jones

doi:10.1007/s00468-015-1239-7

Ectomycorrhizal fungal exoenzyme activity differs on spruce seedlings planted in forests versus clearcuts

Jennifer K. M. Walker^*, Valerie Ward, Melanie D. Jones

^*Corresponding author for this work

Aberdeen Centre For Environmental Sustainability

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

Abstract

Ectomycorrhizal (ECM) fungal community composition is altered by forest harvesting, but it is not clear if this shift in structure influences ECM fungal physiological function at the community level. In this study, we characterized activities of extracellular enzymes in the ectomycorrhizospheres of Picea engelmannii seedlings grown in forest and clearcut plots. These exoenzymes are critical for the breakdown of large organic molecules, from which nutrients are subsequently absorbed and translocated by ECM fungi to host plants. We found that ectomycorrhizae on seedlings planted in forests had different exoenzyme activity profiles than those on seedlings planted in clearcuts. Specifically, the activities of glucuronidase, laccase, and acid phosphatase were higher on forest seedlings (P

Original language	English
Pages (from-to)	497-508
Number of pages	12
Journal	Trees-Structure and function
Volume	30
Issue number	2
Early online date	8 Jul 2015
DOIs	https://doi.org/10.1007/s00468-015-1239-7
Publication status	Published - Apr 2016
Event	6th International Symposium on Physiological Processes in Roots of Woody Plants - Nagoya, Japan Duration: 1 Sept 2014 → 1 Sept 2014

Bibliographical note

This research was supported by a Discovery Grant from the Natural Sciences and Engineering Research Council of Canada and by the Forest Investment Account of the Forest Science Program of the British Columbia (BC) Ministry of Forests, Lands and Natural Resource Operations (MFLNRO) awarded to MJ. JW acknowledges support from the University of British Columbia, Okanagan Campus and the Province of British Columbia for scholarships. We thank Alan Vyse for access to information about the Sicamous Creek field site, MaryAnn Olson, Fawn Ross, and Brendan Twieg for help in the field, and Natasha Lukey and Ayla Fortin for help in the field and lab. Finally, we are immensely grateful to the BC MFLNRO for establishment and long-term maintenance of the Sicamous Creek Silvicultural System Research Project (http://www.for.gov.bc.ca/rsi/research/sicamous/index.htm).

Keywords

Exoenzyme assay
Ectomycorrhizal physiology
Functional complementarity and redundancy
Picea engelmannii
Bioassay seedling
Clearcut harvesting
Community structure
British-Columbia
Enzyme-activity
Soil
Diversity
Microorganisms
Phosphatase
Stands

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Cite this

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title = "Ectomycorrhizal fungal exoenzyme activity differs on spruce seedlings planted in forests versus clearcuts",

abstract = "Ectomycorrhizal (ECM) fungal community composition is altered by forest harvesting, but it is not clear if this shift in structure influences ECM fungal physiological function at the community level. In this study, we characterized activities of extracellular enzymes in the ectomycorrhizospheres of Picea engelmannii seedlings grown in forest and clearcut plots. These exoenzymes are critical for the breakdown of large organic molecules, from which nutrients are subsequently absorbed and translocated by ECM fungi to host plants. We found that ectomycorrhizae on seedlings planted in forests had different exoenzyme activity profiles than those on seedlings planted in clearcuts. Specifically, the activities of glucuronidase, laccase, and acid phosphatase were higher on forest seedlings (P",

keywords = "Exoenzyme assay, Ectomycorrhizal physiology, Functional complementarity and redundancy, Picea engelmannii, Bioassay seedling, Clearcut harvesting, Community structure, British-Columbia, Enzyme-activity, Soil, Diversity, Microorganisms, Phosphatase, Stands",

author = "Walker, {Jennifer K. M.} and Valerie Ward and Jones, {Melanie D.}",

note = "This research was supported by a Discovery Grant from the Natural Sciences and Engineering Research Council of Canada and by the Forest Investment Account of the Forest Science Program of the British Columbia (BC) Ministry of Forests, Lands and Natural Resource Operations (MFLNRO) awarded to MJ. JW acknowledges support from the University of British Columbia, Okanagan Campus and the Province of British Columbia for scholarships. We thank Alan Vyse for access to information about the Sicamous Creek field site, MaryAnn Olson, Fawn Ross, and Brendan Twieg for help in the field, and Natasha Lukey and Ayla Fortin for help in the field and lab. Finally, we are immensely grateful to the BC MFLNRO for establishment and long-term maintenance of the Sicamous Creek Silvicultural System Research Project (http://www.for.gov.bc.ca/rsi/research/sicamous/index.htm).; 6th International Symposium on Physiological Processes in Roots of Woody Plants ; Conference date: 01-09-2014 Through 01-09-2014",

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N2 - Ectomycorrhizal (ECM) fungal community composition is altered by forest harvesting, but it is not clear if this shift in structure influences ECM fungal physiological function at the community level. In this study, we characterized activities of extracellular enzymes in the ectomycorrhizospheres of Picea engelmannii seedlings grown in forest and clearcut plots. These exoenzymes are critical for the breakdown of large organic molecules, from which nutrients are subsequently absorbed and translocated by ECM fungi to host plants. We found that ectomycorrhizae on seedlings planted in forests had different exoenzyme activity profiles than those on seedlings planted in clearcuts. Specifically, the activities of glucuronidase, laccase, and acid phosphatase were higher on forest seedlings (P

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KW - Exoenzyme assay

KW - Ectomycorrhizal physiology

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KW - Bioassay seedling

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KW - British-Columbia

KW - Enzyme-activity

KW - Soil

KW - Diversity

KW - Microorganisms

KW - Phosphatase

KW - Stands

U2 - 10.1007/s00468-015-1239-7

DO - 10.1007/s00468-015-1239-7

M3 - Article

SN - 0931-1890

VL - 30

SP - 497

EP - 508

JO - Trees-Structure and function

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IS - 2

T2 - 6th International Symposium on Physiological Processes in Roots of Woody Plants

Y2 - 1 September 2014 through 1 September 2014

ER -

Ectomycorrhizal fungal exoenzyme activity differs on spruce seedlings planted in forests versus clearcuts

Abstract

Bibliographical note

Keywords

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