Effect of adding acetogenic bacteria on methane production by mixed rumen microorganisms

S. Lopez, F. M. McIntosh, R. J. Wallace, C. J. Newbold

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Six reductive acetogenic bacteria from a variety of ruminal and non-ruminal environments were investigated for their ability to prevent the accumulation of methane when added to rumen fluid incubated in vitro. Acetitomaculum ruminis, Eubacterium limosum strains ATCC 10825 and ATCC 8486, Ruminococcus productus ATCC 35244, and two acetogenic bacteria, Ser 5 and Ser 8 isolated from 20 h old lambs, were grown in media containing glucose. The bacteria retained the ability to produce acetate from H when incubated in the absence of sugar, while no acetate was produced in the absence of H. When cultures of the acetogens were added to incubations of mixed rumen microorganisms in vitro, providing between 0.05 and 0.13 mg of acetogen protein/ml, methane production decreased by about 5% after 24 h with E. limosum ATCC 8486 and Ser 5, while the other bacteria had no effect on methane production. Increasing the concentration of E. limosum ten-fold did not cause a further decrease in methane production. When E. limosum ATCC 8486 and Ser 5 were added to cultures of mixed ruminal microorganisms in the presence of 2-bromoethanesulfonic acid, which inhibited methane formation and caused H to accumulate, both bacteria caused substantial increases in acetate production and decreased H formation. It was concluded that although acetogenic bacteria can utilise H and CO to form acetate in the rumen when methanogenesis is inhibited, even large concentrations of acetogenic bacteria cannot compete for H with methanogenic archaea under normal circumstances.
Original languageEnglish
Pages (from-to)1-9
Number of pages9
JournalAnimal Feed Science and Technology
Volume78
Issue number1-2
DOIs
Publication statusPublished - 31 Mar 1999

Fingerprint

rumen microorganisms
methane production
Eubacterium limosum
bacteria
acetates
Acetitomaculum ruminis
Ruminococcus productus
mixed culture
Archaea
rumen fluids
methane
rumen
lambs
sugars
glucose
acids

Cite this

Effect of adding acetogenic bacteria on methane production by mixed rumen microorganisms. / Lopez, S.; McIntosh, F. M.; Wallace, R. J.; Newbold, C. J.

In: Animal Feed Science and Technology, Vol. 78, No. 1-2, 31.03.1999, p. 1-9.

Research output: Contribution to journalArticle

@article{9f90e4f7eaf94e9c8ae1596da5554ae2,
title = "Effect of adding acetogenic bacteria on methane production by mixed rumen microorganisms",
abstract = "Six reductive acetogenic bacteria from a variety of ruminal and non-ruminal environments were investigated for their ability to prevent the accumulation of methane when added to rumen fluid incubated in vitro. Acetitomaculum ruminis, Eubacterium limosum strains ATCC 10825 and ATCC 8486, Ruminococcus productus ATCC 35244, and two acetogenic bacteria, Ser 5 and Ser 8 isolated from 20 h old lambs, were grown in media containing glucose. The bacteria retained the ability to produce acetate from H when incubated in the absence of sugar, while no acetate was produced in the absence of H. When cultures of the acetogens were added to incubations of mixed rumen microorganisms in vitro, providing between 0.05 and 0.13 mg of acetogen protein/ml, methane production decreased by about 5{\%} after 24 h with E. limosum ATCC 8486 and Ser 5, while the other bacteria had no effect on methane production. Increasing the concentration of E. limosum ten-fold did not cause a further decrease in methane production. When E. limosum ATCC 8486 and Ser 5 were added to cultures of mixed ruminal microorganisms in the presence of 2-bromoethanesulfonic acid, which inhibited methane formation and caused H to accumulate, both bacteria caused substantial increases in acetate production and decreased H formation. It was concluded that although acetogenic bacteria can utilise H and CO to form acetate in the rumen when methanogenesis is inhibited, even large concentrations of acetogenic bacteria cannot compete for H with methanogenic archaea under normal circumstances.",
author = "S. Lopez and McIntosh, {F. M.} and Wallace, {R. J.} and Newbold, {C. J.}",
note = "Copyright 2004 Elsevier Science B.V., Amsterdam. All rights reserved.",
year = "1999",
month = "3",
day = "31",
doi = "10.1016/S0377-8401(98)00273-9",
language = "English",
volume = "78",
pages = "1--9",
journal = "Animal Feed Science and Technology",
issn = "0377-8401",
publisher = "ELSEVIER SCIENCE BV",
number = "1-2",

}

TY - JOUR

T1 - Effect of adding acetogenic bacteria on methane production by mixed rumen microorganisms

AU - Lopez, S.

AU - McIntosh, F. M.

AU - Wallace, R. J.

AU - Newbold, C. J.

N1 - Copyright 2004 Elsevier Science B.V., Amsterdam. All rights reserved.

PY - 1999/3/31

Y1 - 1999/3/31

N2 - Six reductive acetogenic bacteria from a variety of ruminal and non-ruminal environments were investigated for their ability to prevent the accumulation of methane when added to rumen fluid incubated in vitro. Acetitomaculum ruminis, Eubacterium limosum strains ATCC 10825 and ATCC 8486, Ruminococcus productus ATCC 35244, and two acetogenic bacteria, Ser 5 and Ser 8 isolated from 20 h old lambs, were grown in media containing glucose. The bacteria retained the ability to produce acetate from H when incubated in the absence of sugar, while no acetate was produced in the absence of H. When cultures of the acetogens were added to incubations of mixed rumen microorganisms in vitro, providing between 0.05 and 0.13 mg of acetogen protein/ml, methane production decreased by about 5% after 24 h with E. limosum ATCC 8486 and Ser 5, while the other bacteria had no effect on methane production. Increasing the concentration of E. limosum ten-fold did not cause a further decrease in methane production. When E. limosum ATCC 8486 and Ser 5 were added to cultures of mixed ruminal microorganisms in the presence of 2-bromoethanesulfonic acid, which inhibited methane formation and caused H to accumulate, both bacteria caused substantial increases in acetate production and decreased H formation. It was concluded that although acetogenic bacteria can utilise H and CO to form acetate in the rumen when methanogenesis is inhibited, even large concentrations of acetogenic bacteria cannot compete for H with methanogenic archaea under normal circumstances.

AB - Six reductive acetogenic bacteria from a variety of ruminal and non-ruminal environments were investigated for their ability to prevent the accumulation of methane when added to rumen fluid incubated in vitro. Acetitomaculum ruminis, Eubacterium limosum strains ATCC 10825 and ATCC 8486, Ruminococcus productus ATCC 35244, and two acetogenic bacteria, Ser 5 and Ser 8 isolated from 20 h old lambs, were grown in media containing glucose. The bacteria retained the ability to produce acetate from H when incubated in the absence of sugar, while no acetate was produced in the absence of H. When cultures of the acetogens were added to incubations of mixed rumen microorganisms in vitro, providing between 0.05 and 0.13 mg of acetogen protein/ml, methane production decreased by about 5% after 24 h with E. limosum ATCC 8486 and Ser 5, while the other bacteria had no effect on methane production. Increasing the concentration of E. limosum ten-fold did not cause a further decrease in methane production. When E. limosum ATCC 8486 and Ser 5 were added to cultures of mixed ruminal microorganisms in the presence of 2-bromoethanesulfonic acid, which inhibited methane formation and caused H to accumulate, both bacteria caused substantial increases in acetate production and decreased H formation. It was concluded that although acetogenic bacteria can utilise H and CO to form acetate in the rumen when methanogenesis is inhibited, even large concentrations of acetogenic bacteria cannot compete for H with methanogenic archaea under normal circumstances.

UR - http://www.scopus.com/inward/record.url?scp=0033620426&partnerID=8YFLogxK

U2 - 10.1016/S0377-8401(98)00273-9

DO - 10.1016/S0377-8401(98)00273-9

M3 - Article

VL - 78

SP - 1

EP - 9

JO - Animal Feed Science and Technology

JF - Animal Feed Science and Technology

SN - 0377-8401

IS - 1-2

ER -