Effect of anoxia and high sulphide concentrations on heterotrophic microbial communities in reduced surface sediments (black spots) in sandy intertidal flats of the German Wadden Sea.

Thomas Erich Freitag, T. Klenke, W. E. Krumbein, G. Gerdes, James Ivor Prosser

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29 Citations (Scopus)

Abstract

Black reduced sediment surfaces (Black Spots) in sandy intertidal flats of the German Wadden Sea (southern North Sea) are characterised by elevated sulphide concentrations (up to 20 mM) and low redox potentials. It is assumed that the appearance of Black Spots is linked to elevated levels of organic matter content within the sediments. In order to establish the effect of high substrate and sulphide concentrations on the heterotrophic microbial communities in Black Spot sediments, bacteria] abundances and the potential C-source utilisation patterns of microbial communities were compared in natural and artificially induced Black Spots and unaffected control sites. Bacterial numbers were estimated by direct counts and the most probable number technique for different physiological groups, while patterns of C-substrate utilisation of entire aerobic microbial communities were assessed using the Biolog(R), sole-carbon-source-catabolism assay. Bacterial abundances at Black Spot sites were increased, with increases in mean cell numbers, more disperse data distributions and more extreme values. Substrate utilisation patterns of aerobic microbial communities were significantly different in Black Spot sediment slurries, showing diminished richness (number of C-sources catabolised) and substrate diversity (Shannon diversity index) in comparison to unaffected sites. Principal component analysis clearly discriminated Black Spot utilisation patterns from controls and indicated that microbial communities in individual Black Spot sites are functionally diverse and differ from communities in oxidised surface sediments and reduced subsurface sediments at control sites. This work suggests that potentially negative effects on microbial communities in Black Spot sediments, through anoxia and high sulphide concentrations, are balanced by the stimulating influence of substrate availability, leading to comparable or higher bacterial numbers, but lower functional microbial diversity of aerobic microbial communities. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

Original languageEnglish
Pages (from-to)291-301
Number of pages10
JournalFEMS Microbiology Ecology
Volume44
DOIs
Publication statusPublished - 2003

Keywords

  • marine sediment microbial population
  • Biolog (TM)
  • intertidal sediment
  • black spot
  • aerobic functional diversity
  • SOURCE UTILIZATION PATTERNS
  • CARBON-SOURCE UTILIZATION
  • ORGANIC-RICH SEDIMENT
  • SUBSTRATE UTILIZATION
  • SULFATE REDUCTION
  • PERMEABLE SEDIMENTS
  • BACTERIA
  • MATTER
  • MINERALIZATION
  • DEGRADATION

Cite this

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title = "Effect of anoxia and high sulphide concentrations on heterotrophic microbial communities in reduced surface sediments (black spots) in sandy intertidal flats of the German Wadden Sea.",
abstract = "Black reduced sediment surfaces (Black Spots) in sandy intertidal flats of the German Wadden Sea (southern North Sea) are characterised by elevated sulphide concentrations (up to 20 mM) and low redox potentials. It is assumed that the appearance of Black Spots is linked to elevated levels of organic matter content within the sediments. In order to establish the effect of high substrate and sulphide concentrations on the heterotrophic microbial communities in Black Spot sediments, bacteria] abundances and the potential C-source utilisation patterns of microbial communities were compared in natural and artificially induced Black Spots and unaffected control sites. Bacterial numbers were estimated by direct counts and the most probable number technique for different physiological groups, while patterns of C-substrate utilisation of entire aerobic microbial communities were assessed using the Biolog(R), sole-carbon-source-catabolism assay. Bacterial abundances at Black Spot sites were increased, with increases in mean cell numbers, more disperse data distributions and more extreme values. Substrate utilisation patterns of aerobic microbial communities were significantly different in Black Spot sediment slurries, showing diminished richness (number of C-sources catabolised) and substrate diversity (Shannon diversity index) in comparison to unaffected sites. Principal component analysis clearly discriminated Black Spot utilisation patterns from controls and indicated that microbial communities in individual Black Spot sites are functionally diverse and differ from communities in oxidised surface sediments and reduced subsurface sediments at control sites. This work suggests that potentially negative effects on microbial communities in Black Spot sediments, through anoxia and high sulphide concentrations, are balanced by the stimulating influence of substrate availability, leading to comparable or higher bacterial numbers, but lower functional microbial diversity of aerobic microbial communities. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.",
keywords = "marine sediment microbial population, Biolog (TM), intertidal sediment, black spot, aerobic functional diversity, SOURCE UTILIZATION PATTERNS, CARBON-SOURCE UTILIZATION, ORGANIC-RICH SEDIMENT, SUBSTRATE UTILIZATION, SULFATE REDUCTION, PERMEABLE SEDIMENTS, BACTERIA, MATTER, MINERALIZATION, DEGRADATION",
author = "Freitag, {Thomas Erich} and T. Klenke and Krumbein, {W. E.} and G. Gerdes and Prosser, {James Ivor}",
year = "2003",
doi = "10.1016/S0168-6496(03)00076-X",
language = "English",
volume = "44",
pages = "291--301",
journal = "FEMS Microbiology Ecology",
issn = "1574-6941",
publisher = "Oxford University Press",

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TY - JOUR

T1 - Effect of anoxia and high sulphide concentrations on heterotrophic microbial communities in reduced surface sediments (black spots) in sandy intertidal flats of the German Wadden Sea.

AU - Freitag, Thomas Erich

AU - Klenke, T.

AU - Krumbein, W. E.

AU - Gerdes, G.

AU - Prosser, James Ivor

PY - 2003

Y1 - 2003

N2 - Black reduced sediment surfaces (Black Spots) in sandy intertidal flats of the German Wadden Sea (southern North Sea) are characterised by elevated sulphide concentrations (up to 20 mM) and low redox potentials. It is assumed that the appearance of Black Spots is linked to elevated levels of organic matter content within the sediments. In order to establish the effect of high substrate and sulphide concentrations on the heterotrophic microbial communities in Black Spot sediments, bacteria] abundances and the potential C-source utilisation patterns of microbial communities were compared in natural and artificially induced Black Spots and unaffected control sites. Bacterial numbers were estimated by direct counts and the most probable number technique for different physiological groups, while patterns of C-substrate utilisation of entire aerobic microbial communities were assessed using the Biolog(R), sole-carbon-source-catabolism assay. Bacterial abundances at Black Spot sites were increased, with increases in mean cell numbers, more disperse data distributions and more extreme values. Substrate utilisation patterns of aerobic microbial communities were significantly different in Black Spot sediment slurries, showing diminished richness (number of C-sources catabolised) and substrate diversity (Shannon diversity index) in comparison to unaffected sites. Principal component analysis clearly discriminated Black Spot utilisation patterns from controls and indicated that microbial communities in individual Black Spot sites are functionally diverse and differ from communities in oxidised surface sediments and reduced subsurface sediments at control sites. This work suggests that potentially negative effects on microbial communities in Black Spot sediments, through anoxia and high sulphide concentrations, are balanced by the stimulating influence of substrate availability, leading to comparable or higher bacterial numbers, but lower functional microbial diversity of aerobic microbial communities. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

AB - Black reduced sediment surfaces (Black Spots) in sandy intertidal flats of the German Wadden Sea (southern North Sea) are characterised by elevated sulphide concentrations (up to 20 mM) and low redox potentials. It is assumed that the appearance of Black Spots is linked to elevated levels of organic matter content within the sediments. In order to establish the effect of high substrate and sulphide concentrations on the heterotrophic microbial communities in Black Spot sediments, bacteria] abundances and the potential C-source utilisation patterns of microbial communities were compared in natural and artificially induced Black Spots and unaffected control sites. Bacterial numbers were estimated by direct counts and the most probable number technique for different physiological groups, while patterns of C-substrate utilisation of entire aerobic microbial communities were assessed using the Biolog(R), sole-carbon-source-catabolism assay. Bacterial abundances at Black Spot sites were increased, with increases in mean cell numbers, more disperse data distributions and more extreme values. Substrate utilisation patterns of aerobic microbial communities were significantly different in Black Spot sediment slurries, showing diminished richness (number of C-sources catabolised) and substrate diversity (Shannon diversity index) in comparison to unaffected sites. Principal component analysis clearly discriminated Black Spot utilisation patterns from controls and indicated that microbial communities in individual Black Spot sites are functionally diverse and differ from communities in oxidised surface sediments and reduced subsurface sediments at control sites. This work suggests that potentially negative effects on microbial communities in Black Spot sediments, through anoxia and high sulphide concentrations, are balanced by the stimulating influence of substrate availability, leading to comparable or higher bacterial numbers, but lower functional microbial diversity of aerobic microbial communities. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

KW - marine sediment microbial population

KW - Biolog (TM)

KW - intertidal sediment

KW - black spot

KW - aerobic functional diversity

KW - SOURCE UTILIZATION PATTERNS

KW - CARBON-SOURCE UTILIZATION

KW - ORGANIC-RICH SEDIMENT

KW - SUBSTRATE UTILIZATION

KW - SULFATE REDUCTION

KW - PERMEABLE SEDIMENTS

KW - BACTERIA

KW - MATTER

KW - MINERALIZATION

KW - DEGRADATION

U2 - 10.1016/S0168-6496(03)00076-X

DO - 10.1016/S0168-6496(03)00076-X

M3 - Article

VL - 44

SP - 291

EP - 301

JO - FEMS Microbiology Ecology

JF - FEMS Microbiology Ecology

SN - 1574-6941

ER -