Effect of anti-macrophage inflammatory protein-1a on leukocyte trafficking and disease progression in experimental autoimmune uveoretinitis

Isabel Joan Crane, Heping Xu, Ayyakkannu Manivannan, Susan McKillop-Smith, Graeme Robert Lamont, Carol Ann Wallace, Janet Mary Liversidge, Peter Frederick Sharp, John Vincent Forrester

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

This study has enabled us to identify the influence of the chemokine, macrophage inflammatory protein-1alpha (MIP-1alpha), on leukocyte behavior at the blood-retina barrier in vivo and its link with the inflammatory process and disease pathogenesis. MIP-1alpha has not previously been thought to be effective under conditions of physiological shear flow. However, short-term anti-MIP-1alpha treatment inhibited leukocyte slowing and accumulation and subsequent extravasation of leukocytes at the blood-retina barrier in animals with experimental autoimmune uveoretinitis. This was effective predominantly in the post-capillary venules which have been shown to be the main site of passage of leukocytes across the blood-retina barrier. Long-term anti-MIP-1alpha treatment also prevented decreased leukocyte velocity and reduced disease severity as measured clinically, histologically and in terms of blood-retina barrier breakdown.

Original languageEnglish
Pages (from-to)402-410
Number of pages8
JournalEuropean Journal of Immunology
Volume33
Issue number2
DOIs
Publication statusPublished - Feb 2003

Keywords

  • macrophage inflammatory protein-1 alpha
  • CCL3
  • chemokine
  • cell trafficking
  • inflammation
  • ROLLING T-LYMPHOCYTES
  • ENDOTHELIAL-CELLS
  • FLOW CONDITIONS
  • CUTTING EDGE
  • CHEMOKINE
  • ENCEPHALOMYELITIS
  • UVEITIS
  • ARREST
  • MIP-1-ALPHA
  • EXPRESSION

Cite this

Effect of anti-macrophage inflammatory protein-1a on leukocyte trafficking and disease progression in experimental autoimmune uveoretinitis. / Crane, Isabel Joan; Xu, Heping; Manivannan, Ayyakkannu; McKillop-Smith, Susan; Lamont, Graeme Robert; Wallace, Carol Ann; Liversidge, Janet Mary; Sharp, Peter Frederick; Forrester, John Vincent.

In: European Journal of Immunology, Vol. 33, No. 2, 02.2003, p. 402-410.

Research output: Contribution to journalArticle

Crane, Isabel Joan ; Xu, Heping ; Manivannan, Ayyakkannu ; McKillop-Smith, Susan ; Lamont, Graeme Robert ; Wallace, Carol Ann ; Liversidge, Janet Mary ; Sharp, Peter Frederick ; Forrester, John Vincent. / Effect of anti-macrophage inflammatory protein-1a on leukocyte trafficking and disease progression in experimental autoimmune uveoretinitis. In: European Journal of Immunology. 2003 ; Vol. 33, No. 2. pp. 402-410.
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abstract = "This study has enabled us to identify the influence of the chemokine, macrophage inflammatory protein-1alpha (MIP-1alpha), on leukocyte behavior at the blood-retina barrier in vivo and its link with the inflammatory process and disease pathogenesis. MIP-1alpha has not previously been thought to be effective under conditions of physiological shear flow. However, short-term anti-MIP-1alpha treatment inhibited leukocyte slowing and accumulation and subsequent extravasation of leukocytes at the blood-retina barrier in animals with experimental autoimmune uveoretinitis. This was effective predominantly in the post-capillary venules which have been shown to be the main site of passage of leukocytes across the blood-retina barrier. Long-term anti-MIP-1alpha treatment also prevented decreased leukocyte velocity and reduced disease severity as measured clinically, histologically and in terms of blood-retina barrier breakdown.",
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