Effect of antisecretory factor on Escherichia coli STa enterotoxin-induced alkalinisation of pig jejunal acid microclimate

G T McEwan, B Schousboe, E Skadhauge

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The effect of challenge by Escherichia coli STa enterotoxin on pig jejunal mucosal surface pH was investigated in vivo. Exposure to STa resulted in a rapid and reversible alkalinisation (P less than 0.001) of the jejunal mucosa from 6.27 +/- 0.11 (5) to 6.89 +/- 0.03 (5). This action of STa is probably mediated through cyclic 3'5'-guanosine monophosphate (cGMP) since the 8-bromo analogue of cGMP induced the same effect as that observed after STa challenge. The action of STa on mucosal pH was partially inhibited by pre-administration of an antisecretory factor (ASF) preparation. The action of 8-bromo cGMP was unchanged by the presence of ASF. This implies that ASF inhibition occurs during the early stages of STa action prior to stimulation of guanylate cyclase. This effect of STa on the pig jejunal mucosal surface pH, or acid microclimate, may explain why weak acid supplementation of oral rehydration solutions can be ineffective in certain types of diarrhoeal disease.
Original languageEnglish
Pages (from-to)174-9
Number of pages6
JournalPflugers Archiv : European Journal of Physiology
Volume417
Issue number2
Publication statusPublished - Oct 1990

Fingerprint

Microclimate
Escherichia coli
Swine
Acids
Rehydration Solutions
Guanylate Cyclase
Fluid Therapy
Mucous Membrane
heat stable toxin (E coli)
antisecretory factor
3'-guanylic acid
Cyclic GMP

Keywords

  • 8-Bromo Cyclic Adenosine Monophosphate
  • Animals
  • Antidiarrheals
  • Bacterial Toxins
  • Cyclic GMP
  • Enterotoxins
  • Escherichia coli
  • Escherichia coli Proteins
  • Guanylate Cyclase
  • Hydrogen-Ion Concentration
  • Intestinal Mucosa
  • Jejunum
  • Microclimate
  • Neuropeptides
  • Perfusion
  • Swine

Cite this

Effect of antisecretory factor on Escherichia coli STa enterotoxin-induced alkalinisation of pig jejunal acid microclimate. / McEwan, G T; Schousboe, B; Skadhauge, E.

In: Pflugers Archiv : European Journal of Physiology, Vol. 417, No. 2, 10.1990, p. 174-9.

Research output: Contribution to journalArticle

@article{f580a2da58444c909ab28500b0b19587,
title = "Effect of antisecretory factor on Escherichia coli STa enterotoxin-induced alkalinisation of pig jejunal acid microclimate",
abstract = "The effect of challenge by Escherichia coli STa enterotoxin on pig jejunal mucosal surface pH was investigated in vivo. Exposure to STa resulted in a rapid and reversible alkalinisation (P less than 0.001) of the jejunal mucosa from 6.27 +/- 0.11 (5) to 6.89 +/- 0.03 (5). This action of STa is probably mediated through cyclic 3'5'-guanosine monophosphate (cGMP) since the 8-bromo analogue of cGMP induced the same effect as that observed after STa challenge. The action of STa on mucosal pH was partially inhibited by pre-administration of an antisecretory factor (ASF) preparation. The action of 8-bromo cGMP was unchanged by the presence of ASF. This implies that ASF inhibition occurs during the early stages of STa action prior to stimulation of guanylate cyclase. This effect of STa on the pig jejunal mucosal surface pH, or acid microclimate, may explain why weak acid supplementation of oral rehydration solutions can be ineffective in certain types of diarrhoeal disease.",
keywords = "8-Bromo Cyclic Adenosine Monophosphate, Animals, Antidiarrheals, Bacterial Toxins, Cyclic GMP, Enterotoxins, Escherichia coli, Escherichia coli Proteins, Guanylate Cyclase, Hydrogen-Ion Concentration, Intestinal Mucosa, Jejunum, Microclimate, Neuropeptides, Perfusion, Swine",
author = "McEwan, {G T} and B Schousboe and E Skadhauge",
year = "1990",
month = "10",
language = "English",
volume = "417",
pages = "174--9",
journal = "Pflugers Archiv : European Journal of Physiology",
issn = "0031-6768",
publisher = "Springer Verlag",
number = "2",

}

TY - JOUR

T1 - Effect of antisecretory factor on Escherichia coli STa enterotoxin-induced alkalinisation of pig jejunal acid microclimate

AU - McEwan, G T

AU - Schousboe, B

AU - Skadhauge, E

PY - 1990/10

Y1 - 1990/10

N2 - The effect of challenge by Escherichia coli STa enterotoxin on pig jejunal mucosal surface pH was investigated in vivo. Exposure to STa resulted in a rapid and reversible alkalinisation (P less than 0.001) of the jejunal mucosa from 6.27 +/- 0.11 (5) to 6.89 +/- 0.03 (5). This action of STa is probably mediated through cyclic 3'5'-guanosine monophosphate (cGMP) since the 8-bromo analogue of cGMP induced the same effect as that observed after STa challenge. The action of STa on mucosal pH was partially inhibited by pre-administration of an antisecretory factor (ASF) preparation. The action of 8-bromo cGMP was unchanged by the presence of ASF. This implies that ASF inhibition occurs during the early stages of STa action prior to stimulation of guanylate cyclase. This effect of STa on the pig jejunal mucosal surface pH, or acid microclimate, may explain why weak acid supplementation of oral rehydration solutions can be ineffective in certain types of diarrhoeal disease.

AB - The effect of challenge by Escherichia coli STa enterotoxin on pig jejunal mucosal surface pH was investigated in vivo. Exposure to STa resulted in a rapid and reversible alkalinisation (P less than 0.001) of the jejunal mucosa from 6.27 +/- 0.11 (5) to 6.89 +/- 0.03 (5). This action of STa is probably mediated through cyclic 3'5'-guanosine monophosphate (cGMP) since the 8-bromo analogue of cGMP induced the same effect as that observed after STa challenge. The action of STa on mucosal pH was partially inhibited by pre-administration of an antisecretory factor (ASF) preparation. The action of 8-bromo cGMP was unchanged by the presence of ASF. This implies that ASF inhibition occurs during the early stages of STa action prior to stimulation of guanylate cyclase. This effect of STa on the pig jejunal mucosal surface pH, or acid microclimate, may explain why weak acid supplementation of oral rehydration solutions can be ineffective in certain types of diarrhoeal disease.

KW - 8-Bromo Cyclic Adenosine Monophosphate

KW - Animals

KW - Antidiarrheals

KW - Bacterial Toxins

KW - Cyclic GMP

KW - Enterotoxins

KW - Escherichia coli

KW - Escherichia coli Proteins

KW - Guanylate Cyclase

KW - Hydrogen-Ion Concentration

KW - Intestinal Mucosa

KW - Jejunum

KW - Microclimate

KW - Neuropeptides

KW - Perfusion

KW - Swine

M3 - Article

VL - 417

SP - 174

EP - 179

JO - Pflugers Archiv : European Journal of Physiology

JF - Pflugers Archiv : European Journal of Physiology

SN - 0031-6768

IS - 2

ER -