Abstract
Caffeic acid phenethyl ester (CAPE), one of the major components of propolis (honeybee resin), has demonstrated a wide spectrum of activities including suppression of eicosanoids by inhibition of cyclooxygenase-1 and cyclooxygenase-2 enzyme activities. The aim of this study was to investigate the effect of CAPE on basal and secretagogues-stimulated gastric acid secretion in vitro. In the isolated, lumen-perfused, stomach preparation of mouse, CAPE (10-100 microM) did not affect the basal gastric acid secretion nor the secretion stimulated by histamine, pentagastrin, isobutyl methylxanthine and high levels of K+. By contrast, CAPE increased the gastric acid secretion induced by the muscarinic receptor agonist, 5-methylfurmethide (5-MEF). CAPE also inhibited the acetylcholinesterase activity in an in vitro colorimetric assay. Eserine (10 microM), a well known acetylcholinesterase inhibitor, also increased 5-MEF-stimulated acid secretion. Our results show that CAPE increases gastric acid secretion stimulated by an acetylcholine agonist receptor likely through inhibition of acetylcholinesterase activity.
| Original language | English |
|---|---|
| Pages (from-to) | 139-143 |
| Number of pages | 5 |
| Journal | European Journal of Pharmacology |
| Volume | 521 |
| Issue number | 1-3 |
| DOIs | |
| Publication status | Published - 3 Oct 2005 |
Bibliographical note
This work was supported by Regione Campania and Italian MIUR.Keywords
- 1-Methyl-3-isobutylxanthine
- Acetylcholinesterase
- Animals
- Caffeic Acids
- Cholinesterase Inhibitors
- Dose-Response Relationship, Drug
- Gastric Acid
- Gastrointestinal Agents
- Histamine
- In Vitro Techniques
- Male
- Mice
- Mice, Inbred ICR
- Muscarine
- Nifedipine
- Pentagastrin
- Phenylethyl Alcohol
- Physostigmine
- Potassium
- Stomach
- Journal Article
- Research Support, Non-U.S. Gov't