Effect of caffeic acid phenethyl ester on gastric acid secretion in vitro

Francesca Borrelli, Inmaculada Posadas, Raffaele Capasso, Gabriella Aviello, Valeria Ascione, Francesco Capasso

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Caffeic acid phenethyl ester (CAPE), one of the major components of propolis (honeybee resin), has demonstrated a wide spectrum of activities including suppression of eicosanoids by inhibition of cyclooxygenase-1 and cyclooxygenase-2 enzyme activities. The aim of this study was to investigate the effect of CAPE on basal and secretagogues-stimulated gastric acid secretion in vitro. In the isolated, lumen-perfused, stomach preparation of mouse, CAPE (10-100 microM) did not affect the basal gastric acid secretion nor the secretion stimulated by histamine, pentagastrin, isobutyl methylxanthine and high levels of K+. By contrast, CAPE increased the gastric acid secretion induced by the muscarinic receptor agonist, 5-methylfurmethide (5-MEF). CAPE also inhibited the acetylcholinesterase activity in an in vitro colorimetric assay. Eserine (10 microM), a well known acetylcholinesterase inhibitor, also increased 5-MEF-stimulated acid secretion. Our results show that CAPE increases gastric acid secretion stimulated by an acetylcholine agonist receptor likely through inhibition of acetylcholinesterase activity.

Original languageEnglish
Pages (from-to)139-143
Number of pages5
JournalEuropean Journal of Pharmacology
Volume521
Issue number1-3
DOIs
Publication statusPublished - 3 Oct 2005

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Gastric Acid
Acetylcholinesterase
Propolis
Cholinergic Agonists
Muscarinic Agonists
Pentagastrin
Cyclooxygenase 1
Physostigmine
Eicosanoids
Cholinesterase Inhibitors
Cholinergic Receptors
Muscarinic Receptors
Cyclooxygenase 2
Histamine
caffeic acid phenethyl ester
In Vitro Techniques
Stomach
Acids
Enzymes

Keywords

  • 1-Methyl-3-isobutylxanthine
  • Acetylcholinesterase
  • Animals
  • Caffeic Acids
  • Cholinesterase Inhibitors
  • Dose-Response Relationship, Drug
  • Gastric Acid
  • Gastrointestinal Agents
  • Histamine
  • In Vitro Techniques
  • Male
  • Mice
  • Mice, Inbred ICR
  • Muscarine
  • Nifedipine
  • Pentagastrin
  • Phenylethyl Alcohol
  • Physostigmine
  • Potassium
  • Stomach
  • Journal Article
  • Research Support, Non-U.S. Gov't

Cite this

Borrelli, F., Posadas, I., Capasso, R., Aviello, G., Ascione, V., & Capasso, F. (2005). Effect of caffeic acid phenethyl ester on gastric acid secretion in vitro. European Journal of Pharmacology, 521(1-3), 139-143. https://doi.org/10.1016/j.ejphar.2005.08.032

Effect of caffeic acid phenethyl ester on gastric acid secretion in vitro. / Borrelli, Francesca; Posadas, Inmaculada; Capasso, Raffaele; Aviello, Gabriella; Ascione, Valeria; Capasso, Francesco.

In: European Journal of Pharmacology, Vol. 521, No. 1-3, 03.10.2005, p. 139-143.

Research output: Contribution to journalArticle

Borrelli, F, Posadas, I, Capasso, R, Aviello, G, Ascione, V & Capasso, F 2005, 'Effect of caffeic acid phenethyl ester on gastric acid secretion in vitro', European Journal of Pharmacology, vol. 521, no. 1-3, pp. 139-143. https://doi.org/10.1016/j.ejphar.2005.08.032
Borrelli F, Posadas I, Capasso R, Aviello G, Ascione V, Capasso F. Effect of caffeic acid phenethyl ester on gastric acid secretion in vitro. European Journal of Pharmacology. 2005 Oct 3;521(1-3):139-143. https://doi.org/10.1016/j.ejphar.2005.08.032
Borrelli, Francesca ; Posadas, Inmaculada ; Capasso, Raffaele ; Aviello, Gabriella ; Ascione, Valeria ; Capasso, Francesco. / Effect of caffeic acid phenethyl ester on gastric acid secretion in vitro. In: European Journal of Pharmacology. 2005 ; Vol. 521, No. 1-3. pp. 139-143.
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AU - Capasso, Francesco

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AB - Caffeic acid phenethyl ester (CAPE), one of the major components of propolis (honeybee resin), has demonstrated a wide spectrum of activities including suppression of eicosanoids by inhibition of cyclooxygenase-1 and cyclooxygenase-2 enzyme activities. The aim of this study was to investigate the effect of CAPE on basal and secretagogues-stimulated gastric acid secretion in vitro. In the isolated, lumen-perfused, stomach preparation of mouse, CAPE (10-100 microM) did not affect the basal gastric acid secretion nor the secretion stimulated by histamine, pentagastrin, isobutyl methylxanthine and high levels of K+. By contrast, CAPE increased the gastric acid secretion induced by the muscarinic receptor agonist, 5-methylfurmethide (5-MEF). CAPE also inhibited the acetylcholinesterase activity in an in vitro colorimetric assay. Eserine (10 microM), a well known acetylcholinesterase inhibitor, also increased 5-MEF-stimulated acid secretion. Our results show that CAPE increases gastric acid secretion stimulated by an acetylcholine agonist receptor likely through inhibition of acetylcholinesterase activity.

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