Effect of cannabinoids on synaptic transmission in the rat hippocampal slice is temperature-dependent

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Abstract

We have previously reported that the synthetic cannabinoid R-(+)-[2,3-dihydro-5-methyl-3-[(morpholinyl)methyl]pyrrolo[1,2,3-de]-1,4-benzoxazin-yl]-(I-naphthalenyl)methanone mesylate (WIN55,212-2) causes a selective inhibition of paired pulse depression of population spikes recorded from the CA1 region of rat hippocampal slices maintained at 28-30 degreesC. We now show that this effect is highly temperature-dependent and that WIN55,212-2 actually increases paired pulse depression of population spikes recorded from slices maintained at 35 degreesC. This temperature dependence was found to correlate with the effects of the known gamma-amino butyric acid (GABA)-uptake inhibitors, nipecotic acid and guvacine, which were without effect at 28-30 degreesC, but increased paired pulse depression at 35 degreesC. The results show that the effects of cannabinoids on synaptic transmission in the hippocampal slice are highly temperature-dependent and it is suggested that this is due to the presence of increased GABA uptake at higher temperatures. (C) 2002 Elsevier Science B.V. All rights reserved.

Original languageEnglish
Pages (from-to)47-54
Number of pages7
JournalEuropean Journal of Pharmacology
Volume442
Issue number1-2
DOIs
Publication statusPublished - 2002

Keywords

  • WIN55,212-2
  • cannabinoid
  • hippocampus
  • temperature
  • GABA (gamma-aminobutyric acid), uptake
  • PAIRED-PULSE DEPRESSION
  • LONG-TERM POTENTIATION
  • IN-VITRO
  • NEUROTRANSMITTER UPTAKE
  • MEMBRANE-PROPERTIES
  • BRAIN SYNAPTOSOMES
  • GABA(A) RECEPTORS
  • PYRAMIDAL CELLS
  • CB1 RECEPTORS
  • NEURONS

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