Effect of ciprofloxacin on the accumulation of interleukin-6, interleukin-8, and nitrite from a human endothelial cell model of sepsis

Helen F. Galley*, Simon J. Nelson, Anne M. Dubbels, Nigel R. Webster

*Corresponding author for this work

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Objective: To determine the effect of the quinolone antibiotic ciprofloxacin, on interleukin-6, interleukin-8, and nitrite production by human endothelial cells. Design: Controlled cell culture experiments examining the immunomodulatory effects of an antibiotic. Setting: University research laboratory attached to a large teaching hospital. Subjects: A human endothelial cell line. Measurements and Main Results: Cells were incubated with tumor necrosis factor-α and interleukin-1β in the presence of a range of ciprofloxacin concentrations. Interleukin-6, interleukin-8, and nitrite concentrations were measured in culture supernatants after 24 hrs using enzyme immunoassay. Ciprofloxacin decreased interleukin-6 accumulation (p = .001). Interleukin-8 was decreased at lower ciprofloxacin concentrations (p = .017) but was increased at 100 μg/mL (p = .0039). Ciprofloxacin had no effect on nitrite accumulation (p = .38). Conclusions: Ciprofloxacin differentially modulates interleukin 6 and interleukin-8 expression. The response to infection is coordinated by a cascade of cytokines and other mediators. The widespread use of ciprofloxacin in patients with severe infections is likely to result in alterations in local concentrations of cytokines. Selective control of cytokine concentrations by antibacterial agents will clearly have important therapeutic implications and may be a future research consideration in antibiotic drug design.

Original languageEnglish
Pages (from-to)1392-1395
Number of pages4
JournalCritical Care Medicine
Volume25
Issue number8
DOIs
Publication statusPublished - 1 Aug 1997

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Keywords

  • Antibiotics
  • Ciprofloxacin
  • Cytokine
  • Endothelium
  • Interleukin- 8
  • Interleukin-6
  • Nitric oxide
  • Quinolone
  • Sepsis

ASJC Scopus subject areas

  • Critical Care and Intensive Care Medicine

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