Abstract
The presence of adhesion molecules on airway epithelial cells may be important in recruiting leukocytes to the epithelium. The study aimed at investigating the effects of interleukin (IL)-4, IL-8, IL-13 and interferon-gamma (IFN-gamma) on cell viability and intracellular adhesion molecule (ICAM)-1 and zonula occludens protein (ZO)-1 expression on cultured human basal and columnar airway epithelial cells. Cycloheximide (CHX) induced cell death in both cell lines. The cytokines IL-4, IL-8, IL-13 and IFN-gamma had only minor effects on cell proliferation in the columnar 16HBE14o-cells, and inhibited the effects of CHX on cell death. IFN-gamma increased ICAM-1 expression in both cell lines. Western blot analysis showed that CHX inhibited both ICAM-1 and ZO-1 expression in the basal cell line. A combination of IL-4 and IFN-gamma appeared to break the tight junctions. IL-4 and IL-13 potentiated CHX-induced apoptosis in basal cells but not in columnar cells, possibly due to low levels of the IL-4 receptor. It is concluded that cytokines produced by airway epithelium may have a role in regulating sequestering of leukocytes to the airways during airway inflammation.
Original language | English |
---|---|
Pages (from-to) | 768-77 |
Number of pages | 10 |
Journal | Cell Biology International |
Volume | 29 |
Issue number | 9 |
DOIs | |
Publication status | Published - Sept 2005 |
Keywords
- Bronchi
- Cell Adhesion
- Cell Line
- Cell Survival
- Cytokines
- Humans
- Immunohistochemistry
- Intercellular Adhesion Molecule-1
- Interferon-gamma
- Interleukin-13
- Interleukin-4
- Leukocytes
- Membrane Proteins
- Phosphoproteins
- Receptors, Interleukin-4
- Respiratory Mucosa
- Tight Junctions
- Zonula Occludens-1 Protein