Effect of dietary supplementation with glutamine and a combination of glutamine-arginine on intestinal health in twenty-five-day-old weaned rabbits

S. Chamorro, C. de Blas, George Grant, I. Badiola, D. Menoyo, R. Carabano

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The effect of dietary supplementation with 1% L-glutamine and a combination of 1% L-glutamine and 0.5% L-arginine on intestinal health was examined in weaned rabbits. A basal diet was formulated to meet nutrient recommendations. Another 2 diets were formulated by adding 1% (as-fed basis) Gln or a mixture of 1% (as-fed basis) Gln + 0.5% (as-fed basis) Arg (Gln-Arg) to the basal diet. In Exp. 1, a total of 357 rabbits were blocked by litter and assigned at random to the experimental diet to determine mortality ( 119 per diet) and growth performance ( 35 per diet; from weaning at 25 to 56 d of age). Rabbits were fed the experimental diets for a 2-wk period and thereafter received a commercial diet. Rabbits weaned at 25 d ( blocked by litter and assigned at random to diets) were slaughtered at 35 d and used to determine apparent ileal digestibility of DM, CP, and AA ( Exp. 2, a total of 60 rabbits), intestinal morphology, N-aminopeptidase and myeloperoxidase intestinal activity, the expression of PPAR. at the ileum and kidney, serum immunoglobulin in healthy and sick rabbits ( Exp. 3, a total of 24 rabbits), and ileal and cecal microbial composition by PCR-RFLP ( Exp. 4, a total of 45 rabbits). Dietary treatment did not affect ADG, ADFI, or G: F, during the entire fattening period. Supplementation with Gln reduced mortality during the first 2 wk and the whole fattening period from 18.5 to 8.4% ( P = 0.023) and from 31.9 to 20.2% ( P = 0.039), respectively, whereas no effect was detected for Arg supplementation. Among all the variables studied, the reduction on mortality due to Gln was related to a reduced intestinal colonization ( Eimeria lesions) and changes on microbial ecosystem in the ileum and cecum, reducing the frequency of detection of Clostridium spp. ( from 86.7 to 33.3%, P = 0.003) at the ileum, and Helicobacter spp. at the ileum ( from 86.7 to 46.7%, P = 0.003) and at the cecum ( from 86.7 to 46.7, P = 0.028), whereas no effect was detected for Arg supplementation. In conclusion, 1% L-Gln supplementation to postweaned rabbit diets decreased fattening mortality and modified the intestinal microbiota ( although no consistent effects were observed on mucosal histology or inflammatory and systemic immune response). Diets containing a combination of 1% Gln and 0.5% Arg were of little additional benefit.

Original languageEnglish
Pages (from-to)170-180
Number of pages11
JournalJournal of Animal Science
Volume88
Issue number1
Early online date25 Sep 2009
DOIs
Publication statusPublished - Jan 2010

Keywords

  • arginine
  • glutamine
  • intestinal microbiota
  • mucosal integrity
  • rabbit
  • growth-performance
  • barrier function
  • pigs
  • fiber
  • requirements
  • enteropathy
  • metabolism
  • nutrition
  • digestion
  • microflora
  • arginine
  • glutamine
  • intestinal microbiota
  • mucosal integrity

Cite this

Effect of dietary supplementation with glutamine and a combination of glutamine-arginine on intestinal health in twenty-five-day-old weaned rabbits. / Chamorro, S.; de Blas, C.; Grant, George; Badiola, I.; Menoyo, D.; Carabano, R.

In: Journal of Animal Science, Vol. 88, No. 1, 01.2010, p. 170-180.

Research output: Contribution to journalArticle

Chamorro, S. ; de Blas, C. ; Grant, George ; Badiola, I. ; Menoyo, D. ; Carabano, R. / Effect of dietary supplementation with glutamine and a combination of glutamine-arginine on intestinal health in twenty-five-day-old weaned rabbits. In: Journal of Animal Science. 2010 ; Vol. 88, No. 1. pp. 170-180.
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abstract = "The effect of dietary supplementation with 1{\%} L-glutamine and a combination of 1{\%} L-glutamine and 0.5{\%} L-arginine on intestinal health was examined in weaned rabbits. A basal diet was formulated to meet nutrient recommendations. Another 2 diets were formulated by adding 1{\%} (as-fed basis) Gln or a mixture of 1{\%} (as-fed basis) Gln + 0.5{\%} (as-fed basis) Arg (Gln-Arg) to the basal diet. In Exp. 1, a total of 357 rabbits were blocked by litter and assigned at random to the experimental diet to determine mortality ( 119 per diet) and growth performance ( 35 per diet; from weaning at 25 to 56 d of age). Rabbits were fed the experimental diets for a 2-wk period and thereafter received a commercial diet. Rabbits weaned at 25 d ( blocked by litter and assigned at random to diets) were slaughtered at 35 d and used to determine apparent ileal digestibility of DM, CP, and AA ( Exp. 2, a total of 60 rabbits), intestinal morphology, N-aminopeptidase and myeloperoxidase intestinal activity, the expression of PPAR. at the ileum and kidney, serum immunoglobulin in healthy and sick rabbits ( Exp. 3, a total of 24 rabbits), and ileal and cecal microbial composition by PCR-RFLP ( Exp. 4, a total of 45 rabbits). Dietary treatment did not affect ADG, ADFI, or G: F, during the entire fattening period. Supplementation with Gln reduced mortality during the first 2 wk and the whole fattening period from 18.5 to 8.4{\%} ( P = 0.023) and from 31.9 to 20.2{\%} ( P = 0.039), respectively, whereas no effect was detected for Arg supplementation. Among all the variables studied, the reduction on mortality due to Gln was related to a reduced intestinal colonization ( Eimeria lesions) and changes on microbial ecosystem in the ileum and cecum, reducing the frequency of detection of Clostridium spp. ( from 86.7 to 33.3{\%}, P = 0.003) at the ileum, and Helicobacter spp. at the ileum ( from 86.7 to 46.7{\%}, P = 0.003) and at the cecum ( from 86.7 to 46.7, P = 0.028), whereas no effect was detected for Arg supplementation. In conclusion, 1{\%} L-Gln supplementation to postweaned rabbit diets decreased fattening mortality and modified the intestinal microbiota ( although no consistent effects were observed on mucosal histology or inflammatory and systemic immune response). Diets containing a combination of 1{\%} Gln and 0.5{\%} Arg were of little additional benefit.",
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T1 - Effect of dietary supplementation with glutamine and a combination of glutamine-arginine on intestinal health in twenty-five-day-old weaned rabbits

AU - Chamorro, S.

AU - de Blas, C.

AU - Grant, George

AU - Badiola, I.

AU - Menoyo, D.

AU - Carabano, R.

PY - 2010/1

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N2 - The effect of dietary supplementation with 1% L-glutamine and a combination of 1% L-glutamine and 0.5% L-arginine on intestinal health was examined in weaned rabbits. A basal diet was formulated to meet nutrient recommendations. Another 2 diets were formulated by adding 1% (as-fed basis) Gln or a mixture of 1% (as-fed basis) Gln + 0.5% (as-fed basis) Arg (Gln-Arg) to the basal diet. In Exp. 1, a total of 357 rabbits were blocked by litter and assigned at random to the experimental diet to determine mortality ( 119 per diet) and growth performance ( 35 per diet; from weaning at 25 to 56 d of age). Rabbits were fed the experimental diets for a 2-wk period and thereafter received a commercial diet. Rabbits weaned at 25 d ( blocked by litter and assigned at random to diets) were slaughtered at 35 d and used to determine apparent ileal digestibility of DM, CP, and AA ( Exp. 2, a total of 60 rabbits), intestinal morphology, N-aminopeptidase and myeloperoxidase intestinal activity, the expression of PPAR. at the ileum and kidney, serum immunoglobulin in healthy and sick rabbits ( Exp. 3, a total of 24 rabbits), and ileal and cecal microbial composition by PCR-RFLP ( Exp. 4, a total of 45 rabbits). Dietary treatment did not affect ADG, ADFI, or G: F, during the entire fattening period. Supplementation with Gln reduced mortality during the first 2 wk and the whole fattening period from 18.5 to 8.4% ( P = 0.023) and from 31.9 to 20.2% ( P = 0.039), respectively, whereas no effect was detected for Arg supplementation. Among all the variables studied, the reduction on mortality due to Gln was related to a reduced intestinal colonization ( Eimeria lesions) and changes on microbial ecosystem in the ileum and cecum, reducing the frequency of detection of Clostridium spp. ( from 86.7 to 33.3%, P = 0.003) at the ileum, and Helicobacter spp. at the ileum ( from 86.7 to 46.7%, P = 0.003) and at the cecum ( from 86.7 to 46.7, P = 0.028), whereas no effect was detected for Arg supplementation. In conclusion, 1% L-Gln supplementation to postweaned rabbit diets decreased fattening mortality and modified the intestinal microbiota ( although no consistent effects were observed on mucosal histology or inflammatory and systemic immune response). Diets containing a combination of 1% Gln and 0.5% Arg were of little additional benefit.

AB - The effect of dietary supplementation with 1% L-glutamine and a combination of 1% L-glutamine and 0.5% L-arginine on intestinal health was examined in weaned rabbits. A basal diet was formulated to meet nutrient recommendations. Another 2 diets were formulated by adding 1% (as-fed basis) Gln or a mixture of 1% (as-fed basis) Gln + 0.5% (as-fed basis) Arg (Gln-Arg) to the basal diet. In Exp. 1, a total of 357 rabbits were blocked by litter and assigned at random to the experimental diet to determine mortality ( 119 per diet) and growth performance ( 35 per diet; from weaning at 25 to 56 d of age). Rabbits were fed the experimental diets for a 2-wk period and thereafter received a commercial diet. Rabbits weaned at 25 d ( blocked by litter and assigned at random to diets) were slaughtered at 35 d and used to determine apparent ileal digestibility of DM, CP, and AA ( Exp. 2, a total of 60 rabbits), intestinal morphology, N-aminopeptidase and myeloperoxidase intestinal activity, the expression of PPAR. at the ileum and kidney, serum immunoglobulin in healthy and sick rabbits ( Exp. 3, a total of 24 rabbits), and ileal and cecal microbial composition by PCR-RFLP ( Exp. 4, a total of 45 rabbits). Dietary treatment did not affect ADG, ADFI, or G: F, during the entire fattening period. Supplementation with Gln reduced mortality during the first 2 wk and the whole fattening period from 18.5 to 8.4% ( P = 0.023) and from 31.9 to 20.2% ( P = 0.039), respectively, whereas no effect was detected for Arg supplementation. Among all the variables studied, the reduction on mortality due to Gln was related to a reduced intestinal colonization ( Eimeria lesions) and changes on microbial ecosystem in the ileum and cecum, reducing the frequency of detection of Clostridium spp. ( from 86.7 to 33.3%, P = 0.003) at the ileum, and Helicobacter spp. at the ileum ( from 86.7 to 46.7%, P = 0.003) and at the cecum ( from 86.7 to 46.7, P = 0.028), whereas no effect was detected for Arg supplementation. In conclusion, 1% L-Gln supplementation to postweaned rabbit diets decreased fattening mortality and modified the intestinal microbiota ( although no consistent effects were observed on mucosal histology or inflammatory and systemic immune response). Diets containing a combination of 1% Gln and 0.5% Arg were of little additional benefit.

KW - arginine

KW - glutamine

KW - intestinal microbiota

KW - mucosal integrity

KW - rabbit

KW - growth-performance

KW - barrier function

KW - pigs

KW - fiber

KW - requirements

KW - enteropathy

KW - metabolism

KW - nutrition

KW - digestion

KW - microflora

KW - arginine

KW - glutamine

KW - intestinal microbiota

KW - mucosal integrity

U2 - 10.2527/jas.2008-1698

DO - 10.2527/jas.2008-1698

M3 - Article

VL - 88

SP - 170

EP - 180

JO - Journal of Animal Science

JF - Journal of Animal Science

SN - 0021-8812

IS - 1

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