Effect of exogenous nitric oxide and superoxide on interleukin-8 from human polymorphonuclear leucocytes

Brian H. Cuthbertson*, Helen F. Galley, Nigel R. Webster

*Corresponding author for this work

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Patients with acute inflammatory lung injury are commonly treated with inhaled nitric oxide. Nitric oxide has profound immunoregulatory effects. Increased concentrations of the cytokine interleukin-8 (IL-8) in bronchoalveolar lavage fluid has been associated with disease severity. We have investigated the effects of a nitric oxide donor and a combined nitric oxide-superoxide donor on lipopolysaccharide-mediated accumulation of IL-8 from cultured human neutrophils. Interleukin-8 was measured in culture supernatant after 20 h using enzyme immunoassay. The combined nitric oxide-superoxide donor, 3-morpholinosydnonimine dose-dependently decreased lipopolysaccharide-mediated IL-8 accumulation (P < 0.01). SIN-1 also decreased IL-8 accumulation from unstimulated neutrophils (P < 0.001). In contrast, the pure nitric oxide donor, 1,2,3,4-oxatriazolium 5-amino chloride (GEA-3162), increased stimulated IL-8 accumulation (P < 0.01) and also increased IL-8 accumulation in unstimulated cells (P < 0.002). Nitric oxide and superoxide have profound effects on IL-8. These results have important implications for the treatment of patients with acute lung injury with inhaled nitric oxide.

Original languageEnglish
Pages (from-to)714-717
Number of pages4
JournalBritish Journal of Anaesthesia
Volume78
Issue number6
DOIs
Publication statusPublished - 1 Jan 1997

Keywords

  • Lung, adult respiratory distress syndrome
  • Lung, damage
  • Pharmacology, nitric oxide
  • Polypeptides, cytokines

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine

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