Effect of feed intake on amino acid transfers across the ovine hindquarters

S O Hoskin, I C Savary-Auzeloux, Alexander Graham Calder, Grietje Zuur, Gerald Lobley

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Responses in variables of amino acid (AA) metabolism across peripheral tissues to feed intake were studied in six sheep (mean live weight 32 kg) prepared with arterio-venous catheters across the hindquarters. Four intakes (0.5, 1.0, 1.5 and 2.5 x maintenance energy) were offered over 2-week periods to each sheep in a Latin square design with two animals replicated. Animals were infused intravenously with a mixture of U-13C-labelled AA for 10 h and integrated blood samples withdrawn from the aorta and vena cava hourly between 5 and 9 h of infusion. Biopsy samples were also taken from skin and m. vastus lateralis. Data from both essential (histidine, isoleucine, leucine, lysine, phenylalanine, threonine) and nonessential (glycine, proline, serine, tyrosine) AA were modelled to give rates of inward and outward transport, protein synthesis and degradation, plus the fraction of total vascular inflow that exchanged with the hindquarter tissues. Rates of inward transport varied more than 10-fold between AA. For all essential AA (plus serine), inward transport increased with food intake (P
Original languageEnglish
Pages (from-to)167-179
Number of pages13
JournalBritish Journal of Nutrition
Volume89
Issue number2
DOIs
Publication statusPublished - 1 Feb 2003

Fingerprint

Sheep
Amino Acids
Serine
Venae Cavae
Essential Amino Acids
Isoleucine
Quadriceps Muscle
Threonine
Phenylalanine
Histidine
Proline
Leucine
Glycine
Proteolysis
Lysine
Blood Vessels
Tyrosine
Aorta
Carrier Proteins
Catheters

Keywords

  • Amino Acids
  • Animal Feed
  • Animals
  • Carbon Isotopes
  • Eating
  • Hindlimb
  • Male
  • Muscle, Skeletal
  • Sheep
  • Skin
  • p-Aminohippuric Acid

Cite this

Effect of feed intake on amino acid transfers across the ovine hindquarters. / Hoskin, S O; Savary-Auzeloux, I C; Calder, Alexander Graham; Zuur, Grietje; Lobley, Gerald.

In: British Journal of Nutrition, Vol. 89, No. 2, 01.02.2003, p. 167-179.

Research output: Contribution to journalArticle

Hoskin, S O ; Savary-Auzeloux, I C ; Calder, Alexander Graham ; Zuur, Grietje ; Lobley, Gerald. / Effect of feed intake on amino acid transfers across the ovine hindquarters. In: British Journal of Nutrition. 2003 ; Vol. 89, No. 2. pp. 167-179.
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