Effect of fluoride substitution on the biocompatibility of hydroxyapatite

K A Hing, L Di Silvio, I R Gibson, C Ohtsuki, L J Jha, S M Best, W Bonfield

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Hydroxyapatite (HA) and 0.2 and 0.3 wt% fluoride-substituted apatite (F-HA) were studied using in vitro testing by both immersion in simulated body-fluid and direct cell culture on ceramic substrates. X-ray diffraction and scanning electron microscopy revealed the formation of a bonelike apatite layer on F-HA discs after only 3 days immersion in simulated body-fluid, compared to 14 days on HA discs. The increased activity of the substituted apatite was confirmed by in vitro assessment using a primary human osteoblast-like (HOB) cell line. HOB cells cultured on the substituted apatites demonstrated elevated levels of tritiated thymidine incorporation compared to pure HA. However, alkaline phosphatase expression after 7 days in vitro was found to be independent of substrate composition, indicating that fluoride substitution did not affect the phenotypic expression of the HOB cells. Scanning electron microscopy of the cultures after 1 day in vitro demonstrated formation of a bone-like apatite layer on the surface of the 0.3wt% F-HA, and a protein-like layer was identified on both 0.2 and 0.3 wt% F-HA discs after 3 days in vitro.

Original languageEnglish
Title of host publicationBIOCERAMICS, VOL 10
EditorsL Sedel, C Rey
Place of PublicationOXFORD
PublisherPERGAMON PRESS LTD
Pages19-22
Number of pages4
ISBN (Print)0-08-042692-1
Publication statusPublished - 1997
Event10th International Symposium on Ceramics in Medicine - PARIS
Duration: 5 Oct 19979 Oct 1997

Conference

Conference10th International Symposium on Ceramics in Medicine
CityPARIS
Period5/10/979/10/97

Cite this

Hing, K. A., Di Silvio, L., Gibson, I. R., Ohtsuki, C., Jha, L. J., Best, S. M., & Bonfield, W. (1997). Effect of fluoride substitution on the biocompatibility of hydroxyapatite. In L. Sedel, & C. Rey (Eds.), BIOCERAMICS, VOL 10 (pp. 19-22). OXFORD: PERGAMON PRESS LTD.

Effect of fluoride substitution on the biocompatibility of hydroxyapatite. / Hing, K A ; Di Silvio, L ; Gibson, I R ; Ohtsuki, C ; Jha, L J ; Best, S M ; Bonfield, W .

BIOCERAMICS, VOL 10. ed. / L Sedel; C Rey. OXFORD : PERGAMON PRESS LTD, 1997. p. 19-22.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Hing, KA, Di Silvio, L, Gibson, IR, Ohtsuki, C, Jha, LJ, Best, SM & Bonfield, W 1997, Effect of fluoride substitution on the biocompatibility of hydroxyapatite. in L Sedel & C Rey (eds), BIOCERAMICS, VOL 10. PERGAMON PRESS LTD, OXFORD, pp. 19-22, 10th International Symposium on Ceramics in Medicine, PARIS, 5/10/97.
Hing KA, Di Silvio L, Gibson IR, Ohtsuki C, Jha LJ, Best SM et al. Effect of fluoride substitution on the biocompatibility of hydroxyapatite. In Sedel L, Rey C, editors, BIOCERAMICS, VOL 10. OXFORD: PERGAMON PRESS LTD. 1997. p. 19-22
Hing, K A ; Di Silvio, L ; Gibson, I R ; Ohtsuki, C ; Jha, L J ; Best, S M ; Bonfield, W . / Effect of fluoride substitution on the biocompatibility of hydroxyapatite. BIOCERAMICS, VOL 10. editor / L Sedel ; C Rey. OXFORD : PERGAMON PRESS LTD, 1997. pp. 19-22
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AU - Best, S M

AU - Bonfield, W

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N2 - Hydroxyapatite (HA) and 0.2 and 0.3 wt% fluoride-substituted apatite (F-HA) were studied using in vitro testing by both immersion in simulated body-fluid and direct cell culture on ceramic substrates. X-ray diffraction and scanning electron microscopy revealed the formation of a bonelike apatite layer on F-HA discs after only 3 days immersion in simulated body-fluid, compared to 14 days on HA discs. The increased activity of the substituted apatite was confirmed by in vitro assessment using a primary human osteoblast-like (HOB) cell line. HOB cells cultured on the substituted apatites demonstrated elevated levels of tritiated thymidine incorporation compared to pure HA. However, alkaline phosphatase expression after 7 days in vitro was found to be independent of substrate composition, indicating that fluoride substitution did not affect the phenotypic expression of the HOB cells. Scanning electron microscopy of the cultures after 1 day in vitro demonstrated formation of a bone-like apatite layer on the surface of the 0.3wt% F-HA, and a protein-like layer was identified on both 0.2 and 0.3 wt% F-HA discs after 3 days in vitro.

AB - Hydroxyapatite (HA) and 0.2 and 0.3 wt% fluoride-substituted apatite (F-HA) were studied using in vitro testing by both immersion in simulated body-fluid and direct cell culture on ceramic substrates. X-ray diffraction and scanning electron microscopy revealed the formation of a bonelike apatite layer on F-HA discs after only 3 days immersion in simulated body-fluid, compared to 14 days on HA discs. The increased activity of the substituted apatite was confirmed by in vitro assessment using a primary human osteoblast-like (HOB) cell line. HOB cells cultured on the substituted apatites demonstrated elevated levels of tritiated thymidine incorporation compared to pure HA. However, alkaline phosphatase expression after 7 days in vitro was found to be independent of substrate composition, indicating that fluoride substitution did not affect the phenotypic expression of the HOB cells. Scanning electron microscopy of the cultures after 1 day in vitro demonstrated formation of a bone-like apatite layer on the surface of the 0.3wt% F-HA, and a protein-like layer was identified on both 0.2 and 0.3 wt% F-HA discs after 3 days in vitro.

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