Effect of forage conservation method on ruminal lipid metabolism and microbial ecology in lactating cows fed diets containing a 60:40 forage-to-concentrate ratio

A. Halmemies-Beauchet-Filleau, P. Kairenius, S. Ahvenjarvi, L. K. Crosley, S. Muetzel, P. Huhtanen, A. Vanhatalo, V. Toivonen, R. J. Wallace, K. J. Shingfield

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The effect of forage conservation method on ruminal lipid metabolism and microbial ecology was examined in 2 complementary experiments in cows. Treatments comprised fresh chopped grass, barn-dried hay, or untreated (UTS) or formic acid-treated silage (FAS) prepared from the same grass sward. Preparation of conserved forages coincided with the collection of samples from cows offered fresh grass. In the first experiment, 5 multiparous Finnish Ayrshire cows (229 d in milk) were used to compare the effects of feeding diets based on grass followed by hay during 2 consecutive 14-d periods separated by a 5-d transition during which extensively wilted grass was fed. In the second experiment, 5 multiparous Finnish Ayrshire cows (53 d in milk) were assigned to 1 of 2 blocks and allocated treatments according to a replicated 3 x 3 Latin square design with 14-d periods to compare the effects of hay, UTS, and FAS. Cows received 7 or 9 kg/d of the same concentrate in experiments 1 and 2, respectively. Conservation of grass by drying, but not ensiling, decreased forage fatty acid content primarily due to losses of 18:2n-6 and 18:3n-3. Compared with grass, feeding hay had no effect on dry matter intake (DMI), rumen pH, or fermentation characteristics, other than increasing ammonia content, but lowered whole-tract organic matter and fiber digestibility (experiment 1). Relative to hay, silage increased DMI, rumen volatile fatty acid (VFA) concentrations, and molar proportions of butyrate, and decreased molar acetate proportions (experiment 2). Compared with UTS, FAS increased DMI, had no effect on rumen ammonia or VFA concentrations, but tended to lower rumen pH and the molar ratio of lipogenic to glucogenic VFA. Conservation method had no substantial effect on ruminal or whole-tract digestibility coefficients. Compared with fresh grass and silages, hay decreased lipolysis and biohydrogenation (BH) of dietary unsaturates in the rumen, resulting in similar flows of 18:2n-6 and 18:3n-3, but lower amounts of trans-11 18:1 and Delta11,13 18:2 at the omasum. The extent of silage fermentation had minimal influence on ruminal lipid metabolism. Treatments were not associated with changes in the relative abundance of specific bacteria known to be capable of BH or rumen protozoal numbers. In conclusion, conservation method altered forage lipids, the extent of lipolysis and BH in the rumen, and the flow of fatty acids at the omasum, in the absence of substantial changes in ruminal Butyrivibrio populations.
Original languageEnglish
Pages (from-to)2428-2447
Number of pages20
JournalJournal of Dairy Science
Volume96
Issue number4
DOIs
Publication statusPublished - Apr 2013

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microbial ecology
Poaceae
Ecology
Lipid Metabolism
Rumen
lipid metabolism
formic acid
Silage
rumen
concentrates
hay
forage
Diet
grasses
silage
cows
biohydrogenation
diet
Volatile Fatty Acids
volatile fatty acids

Keywords

  • butyrivibrio
  • cow
  • forage
  • conservation
  • ruminal biohydrogenation

Cite this

Halmemies-Beauchet-Filleau, A., Kairenius, P., Ahvenjarvi, S., Crosley, L. K., Muetzel, S., Huhtanen, P., ... Shingfield, K. J. (2013). Effect of forage conservation method on ruminal lipid metabolism and microbial ecology in lactating cows fed diets containing a 60:40 forage-to-concentrate ratio. Journal of Dairy Science, 96(4), 2428-2447. https://doi.org/10.3168/jds.2012-6043

Effect of forage conservation method on ruminal lipid metabolism and microbial ecology in lactating cows fed diets containing a 60:40 forage-to-concentrate ratio. / Halmemies-Beauchet-Filleau, A.; Kairenius, P.; Ahvenjarvi, S.; Crosley, L. K.; Muetzel, S.; Huhtanen, P.; Vanhatalo, A.; Toivonen, V.; Wallace, R. J.; Shingfield, K. J.

In: Journal of Dairy Science, Vol. 96, No. 4, 04.2013, p. 2428-2447.

Research output: Contribution to journalArticle

Halmemies-Beauchet-Filleau, A, Kairenius, P, Ahvenjarvi, S, Crosley, LK, Muetzel, S, Huhtanen, P, Vanhatalo, A, Toivonen, V, Wallace, RJ & Shingfield, KJ 2013, 'Effect of forage conservation method on ruminal lipid metabolism and microbial ecology in lactating cows fed diets containing a 60:40 forage-to-concentrate ratio', Journal of Dairy Science, vol. 96, no. 4, pp. 2428-2447. https://doi.org/10.3168/jds.2012-6043
Halmemies-Beauchet-Filleau, A. ; Kairenius, P. ; Ahvenjarvi, S. ; Crosley, L. K. ; Muetzel, S. ; Huhtanen, P. ; Vanhatalo, A. ; Toivonen, V. ; Wallace, R. J. ; Shingfield, K. J. / Effect of forage conservation method on ruminal lipid metabolism and microbial ecology in lactating cows fed diets containing a 60:40 forage-to-concentrate ratio. In: Journal of Dairy Science. 2013 ; Vol. 96, No. 4. pp. 2428-2447.
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AU - Kairenius, P.

AU - Ahvenjarvi, S.

AU - Crosley, L. K.

AU - Muetzel, S.

AU - Huhtanen, P.

AU - Vanhatalo, A.

AU - Toivonen, V.

AU - Wallace, R. J.

AU - Shingfield, K. J.

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AB - The effect of forage conservation method on ruminal lipid metabolism and microbial ecology was examined in 2 complementary experiments in cows. Treatments comprised fresh chopped grass, barn-dried hay, or untreated (UTS) or formic acid-treated silage (FAS) prepared from the same grass sward. Preparation of conserved forages coincided with the collection of samples from cows offered fresh grass. In the first experiment, 5 multiparous Finnish Ayrshire cows (229 d in milk) were used to compare the effects of feeding diets based on grass followed by hay during 2 consecutive 14-d periods separated by a 5-d transition during which extensively wilted grass was fed. In the second experiment, 5 multiparous Finnish Ayrshire cows (53 d in milk) were assigned to 1 of 2 blocks and allocated treatments according to a replicated 3 x 3 Latin square design with 14-d periods to compare the effects of hay, UTS, and FAS. Cows received 7 or 9 kg/d of the same concentrate in experiments 1 and 2, respectively. Conservation of grass by drying, but not ensiling, decreased forage fatty acid content primarily due to losses of 18:2n-6 and 18:3n-3. Compared with grass, feeding hay had no effect on dry matter intake (DMI), rumen pH, or fermentation characteristics, other than increasing ammonia content, but lowered whole-tract organic matter and fiber digestibility (experiment 1). Relative to hay, silage increased DMI, rumen volatile fatty acid (VFA) concentrations, and molar proportions of butyrate, and decreased molar acetate proportions (experiment 2). Compared with UTS, FAS increased DMI, had no effect on rumen ammonia or VFA concentrations, but tended to lower rumen pH and the molar ratio of lipogenic to glucogenic VFA. Conservation method had no substantial effect on ruminal or whole-tract digestibility coefficients. Compared with fresh grass and silages, hay decreased lipolysis and biohydrogenation (BH) of dietary unsaturates in the rumen, resulting in similar flows of 18:2n-6 and 18:3n-3, but lower amounts of trans-11 18:1 and Delta11,13 18:2 at the omasum. The extent of silage fermentation had minimal influence on ruminal lipid metabolism. Treatments were not associated with changes in the relative abundance of specific bacteria known to be capable of BH or rumen protozoal numbers. In conclusion, conservation method altered forage lipids, the extent of lipolysis and BH in the rumen, and the flow of fatty acids at the omasum, in the absence of substantial changes in ruminal Butyrivibrio populations.

KW - butyrivibrio

KW - cow

KW - forage

KW - conservation

KW - ruminal biohydrogenation

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DO - 10.3168/jds.2012-6043

M3 - Article

VL - 96

SP - 2428

EP - 2447

JO - Journal of Dairy Science

JF - Journal of Dairy Science

SN - 0022-0302

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ER -