Effect of hyaluronan oligosaccharides on the expression of heat shock protein 72*

Heping Xu, T. Ito, A. Tawada, H. Maeda, H. Ymanokuchi, K. Isahara, K. Yoshida, Y. Uchiyama, A. Asari

    Research output: Contribution to journalArticle

    88 Citations (Scopus)

    Abstract

    We have previously shown that intraarticular treatment with a hyaluronan (HA) preparation (840 kDa), HA84, up-regulates heat shock protein 72 (Hsp72) expression and suppresses degeneration of synovial cells in an arthritis model. In that study, the RA84 administered was degraded into HA oligosaccharides in the synovial tissue, suggesting that HA84 or degradation products of RA may up-regulate Hsp72 expression. Thus, in the present study, we examined the effects of HA of various molecular sizes on Hsp72 expression and cell death in stressed cells. Western blotting analysis showed that treatment of K562 cells with HA tetrasaccharides up-regulated Hsp72 expression after exposure to hyperthermia. On the other hand, treatment of the cells with HA of other sizes (di-, hexa-, deca-, dodecasaccharides), HA84, or tetrasaccharides of keratan sulfate did not elicit any change in expression of the Hsp72 protein. Treatment of the cells with tetrasaccharides of HA up-regulated not only expression of the Hsp72 protein but also Hsp72 mRNA expression and enhanced activation of HSF1, a transcription factor controlling Hsp72 expression, after exposure to hyperthermia. Because the level of Hsp72 protein was not affected by tetrasaccharides of RA when the K562 cells were kept at 37 degreesC without any stress, it is evident that tetrasaccharides of HA did not act as a stress factor. In addition, tetrasaccharides of HA suppressed cell death in the case of K562 cells exposed to hyperthermia and of PC12 cells under serum deprivation. These results suggest that a certain size of oligosaccharides, i.e. the tetrasaccharides of HA, up-regulates Hsp72 expression by enhancing the activation of HSF1 under stress conditions and suppresses cell death.

    Original languageEnglish
    Pages (from-to)17308-17314
    Number of pages6
    JournalThe Journal of Biological Chemistry
    Volume277
    Issue number19
    DOIs
    Publication statusPublished - May 2002

    Keywords

    • HEAT-SHOCK RESPONSE
    • STRESS-INDUCED APOPTOSIS
    • GENE-TRANSCRIPTION
    • FACTOR-I
    • PROTEIN
    • CELLS
    • ACID
    • OSTEOARTHRITIS
    • ACTIVATION
    • OLIGOMERIZATION

    Cite this

    Xu, H., Ito, T., Tawada, A., Maeda, H., Ymanokuchi, H., Isahara, K., ... Asari, A. (2002). Effect of hyaluronan oligosaccharides on the expression of heat shock protein 72*. The Journal of Biological Chemistry, 277(19), 17308-17314. https://doi.org/10.1074/jbc.M112371200

    Effect of hyaluronan oligosaccharides on the expression of heat shock protein 72*. / Xu, Heping; Ito, T.; Tawada, A.; Maeda, H.; Ymanokuchi, H.; Isahara, K.; Yoshida, K.; Uchiyama, Y.; Asari, A.

    In: The Journal of Biological Chemistry, Vol. 277, No. 19, 05.2002, p. 17308-17314.

    Research output: Contribution to journalArticle

    Xu, H, Ito, T, Tawada, A, Maeda, H, Ymanokuchi, H, Isahara, K, Yoshida, K, Uchiyama, Y & Asari, A 2002, 'Effect of hyaluronan oligosaccharides on the expression of heat shock protein 72*' The Journal of Biological Chemistry, vol. 277, no. 19, pp. 17308-17314. https://doi.org/10.1074/jbc.M112371200
    Xu, Heping ; Ito, T. ; Tawada, A. ; Maeda, H. ; Ymanokuchi, H. ; Isahara, K. ; Yoshida, K. ; Uchiyama, Y. ; Asari, A. / Effect of hyaluronan oligosaccharides on the expression of heat shock protein 72*. In: The Journal of Biological Chemistry. 2002 ; Vol. 277, No. 19. pp. 17308-17314.
    @article{14aeaee94da9459f945c1a2a9cb96a1c,
    title = "Effect of hyaluronan oligosaccharides on the expression of heat shock protein 72*",
    abstract = "We have previously shown that intraarticular treatment with a hyaluronan (HA) preparation (840 kDa), HA84, up-regulates heat shock protein 72 (Hsp72) expression and suppresses degeneration of synovial cells in an arthritis model. In that study, the RA84 administered was degraded into HA oligosaccharides in the synovial tissue, suggesting that HA84 or degradation products of RA may up-regulate Hsp72 expression. Thus, in the present study, we examined the effects of HA of various molecular sizes on Hsp72 expression and cell death in stressed cells. Western blotting analysis showed that treatment of K562 cells with HA tetrasaccharides up-regulated Hsp72 expression after exposure to hyperthermia. On the other hand, treatment of the cells with HA of other sizes (di-, hexa-, deca-, dodecasaccharides), HA84, or tetrasaccharides of keratan sulfate did not elicit any change in expression of the Hsp72 protein. Treatment of the cells with tetrasaccharides of HA up-regulated not only expression of the Hsp72 protein but also Hsp72 mRNA expression and enhanced activation of HSF1, a transcription factor controlling Hsp72 expression, after exposure to hyperthermia. Because the level of Hsp72 protein was not affected by tetrasaccharides of RA when the K562 cells were kept at 37 degreesC without any stress, it is evident that tetrasaccharides of HA did not act as a stress factor. In addition, tetrasaccharides of HA suppressed cell death in the case of K562 cells exposed to hyperthermia and of PC12 cells under serum deprivation. These results suggest that a certain size of oligosaccharides, i.e. the tetrasaccharides of HA, up-regulates Hsp72 expression by enhancing the activation of HSF1 under stress conditions and suppresses cell death.",
    keywords = "HEAT-SHOCK RESPONSE, STRESS-INDUCED APOPTOSIS, GENE-TRANSCRIPTION, FACTOR-I, PROTEIN, CELLS, ACID, OSTEOARTHRITIS, ACTIVATION, OLIGOMERIZATION",
    author = "Heping Xu and T. Ito and A. Tawada and H. Maeda and H. Ymanokuchi and K. Isahara and K. Yoshida and Y. Uchiyama and A. Asari",
    year = "2002",
    month = "5",
    doi = "10.1074/jbc.M112371200",
    language = "English",
    volume = "277",
    pages = "17308--17314",
    journal = "The Journal of Biological Chemistry",
    issn = "0021-9258",
    publisher = "AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC",
    number = "19",

    }

    TY - JOUR

    T1 - Effect of hyaluronan oligosaccharides on the expression of heat shock protein 72*

    AU - Xu, Heping

    AU - Ito, T.

    AU - Tawada, A.

    AU - Maeda, H.

    AU - Ymanokuchi, H.

    AU - Isahara, K.

    AU - Yoshida, K.

    AU - Uchiyama, Y.

    AU - Asari, A.

    PY - 2002/5

    Y1 - 2002/5

    N2 - We have previously shown that intraarticular treatment with a hyaluronan (HA) preparation (840 kDa), HA84, up-regulates heat shock protein 72 (Hsp72) expression and suppresses degeneration of synovial cells in an arthritis model. In that study, the RA84 administered was degraded into HA oligosaccharides in the synovial tissue, suggesting that HA84 or degradation products of RA may up-regulate Hsp72 expression. Thus, in the present study, we examined the effects of HA of various molecular sizes on Hsp72 expression and cell death in stressed cells. Western blotting analysis showed that treatment of K562 cells with HA tetrasaccharides up-regulated Hsp72 expression after exposure to hyperthermia. On the other hand, treatment of the cells with HA of other sizes (di-, hexa-, deca-, dodecasaccharides), HA84, or tetrasaccharides of keratan sulfate did not elicit any change in expression of the Hsp72 protein. Treatment of the cells with tetrasaccharides of HA up-regulated not only expression of the Hsp72 protein but also Hsp72 mRNA expression and enhanced activation of HSF1, a transcription factor controlling Hsp72 expression, after exposure to hyperthermia. Because the level of Hsp72 protein was not affected by tetrasaccharides of RA when the K562 cells were kept at 37 degreesC without any stress, it is evident that tetrasaccharides of HA did not act as a stress factor. In addition, tetrasaccharides of HA suppressed cell death in the case of K562 cells exposed to hyperthermia and of PC12 cells under serum deprivation. These results suggest that a certain size of oligosaccharides, i.e. the tetrasaccharides of HA, up-regulates Hsp72 expression by enhancing the activation of HSF1 under stress conditions and suppresses cell death.

    AB - We have previously shown that intraarticular treatment with a hyaluronan (HA) preparation (840 kDa), HA84, up-regulates heat shock protein 72 (Hsp72) expression and suppresses degeneration of synovial cells in an arthritis model. In that study, the RA84 administered was degraded into HA oligosaccharides in the synovial tissue, suggesting that HA84 or degradation products of RA may up-regulate Hsp72 expression. Thus, in the present study, we examined the effects of HA of various molecular sizes on Hsp72 expression and cell death in stressed cells. Western blotting analysis showed that treatment of K562 cells with HA tetrasaccharides up-regulated Hsp72 expression after exposure to hyperthermia. On the other hand, treatment of the cells with HA of other sizes (di-, hexa-, deca-, dodecasaccharides), HA84, or tetrasaccharides of keratan sulfate did not elicit any change in expression of the Hsp72 protein. Treatment of the cells with tetrasaccharides of HA up-regulated not only expression of the Hsp72 protein but also Hsp72 mRNA expression and enhanced activation of HSF1, a transcription factor controlling Hsp72 expression, after exposure to hyperthermia. Because the level of Hsp72 protein was not affected by tetrasaccharides of RA when the K562 cells were kept at 37 degreesC without any stress, it is evident that tetrasaccharides of HA did not act as a stress factor. In addition, tetrasaccharides of HA suppressed cell death in the case of K562 cells exposed to hyperthermia and of PC12 cells under serum deprivation. These results suggest that a certain size of oligosaccharides, i.e. the tetrasaccharides of HA, up-regulates Hsp72 expression by enhancing the activation of HSF1 under stress conditions and suppresses cell death.

    KW - HEAT-SHOCK RESPONSE

    KW - STRESS-INDUCED APOPTOSIS

    KW - GENE-TRANSCRIPTION

    KW - FACTOR-I

    KW - PROTEIN

    KW - CELLS

    KW - ACID

    KW - OSTEOARTHRITIS

    KW - ACTIVATION

    KW - OLIGOMERIZATION

    U2 - 10.1074/jbc.M112371200

    DO - 10.1074/jbc.M112371200

    M3 - Article

    VL - 277

    SP - 17308

    EP - 17314

    JO - The Journal of Biological Chemistry

    JF - The Journal of Biological Chemistry

    SN - 0021-9258

    IS - 19

    ER -