Effect of increasing fruit and vegetable intake by dietary intervention on nutritional biomarkers and attitudes to dietary change

a randomised trial

Susan J. Duthie, Garry G. Duthie, Wendy R. Russell, Janet A. M. Kyle, Jennie I. Macdiarmid, Vanessa Rungapamestry, Sylvia Stephen, Cristina Megias-Baeza, Joanna J. Kaniewska, Lindsey Shaw, Lesley Milne, David Bremner, Karen Ross, Philip Morrice, Lynn P. Pirie, Graham Horgan, Charles S. Bestwick

Research output: Contribution to journalArticle

4 Citations (Scopus)
6 Downloads (Pure)

Abstract

PURPOSE: Low fruit and vegetable consumption is linked with an increased risk of death from vascular disease and cancer. The benefit of eating fruits and vegetables is attributed in part to antioxidants, vitamins and phytochemicals. Whether increasing intake impacts on markers of disease remains to be established. This study investigates whether increasing daily intake of fruits, vegetables and juices from low (approx. 3 portions), to high intakes (approx. 8 portions) impacts on nutritional and clinical biomarkers. Barriers to achieving the recommended fruit and vegetable intakes are also investigated.

METHOD: In a randomised clinical trial, the participants [19 men and 26 women (39-58 years)] with low reported fruit, juice and vegetable intake (<3 portions/day) were randomised to consume either their usual diet or a diet supplemented with an additional 480 g of fruit and vegetables and fruit juice (300 ml) daily for 12 weeks. Nutritional biomarkers (vitamin C, carotenoids, B vitamins), antioxidant capacity and genomic stability were measured pre-intervention, at 4-, 8- and 12 weeks throughout the intervention. Samples were also taken post-intervention after a 6-week washout period. Glucose, homocysteine, lipids, blood pressure, weight and arterial stiffness were also measured. Intake of fruit, fruit juice and vegetables was reassessed 12 months after conducting the study and a questionnaire was developed to identify barriers to healthy eating.

RESULTS: Intake increased significantly in the intervention group compared to controls, achieving 8.4 portions/day after 12 weeks. Plasma vitamin C (35%), folate (15%) and certain carotenoids [α-carotene (50%) and β-carotene (70%) and lutein/zeaxanthin (70%)] were significantly increased (P < 0.05) in the intervention group. There were no significant changes in antioxidant capacity, DNA damage and markers of vascular health. Barriers to achieving recommended intakes of fruits and vegetables measured 12 months after the intervention period were amount, inconvenience and cost.

CONCLUSION: While increasing fruit, juice and vegetable consumption increases circulating level of beneficial nutrients in healthy subjects, a 12-week intervention was not associated with effects on antioxidant status or lymphocyte DNA damage.

TRIAL REGISTRATION: This trial was registered at Controlled-Trials.com; registration ISRCTN71368072.

Original languageEnglish
Pages (from-to)1855-1872
Number of pages18
JournalEuropean Journal of Nutrition
Volume57
Issue number5
Early online date30 May 2017
DOIs
Publication statusPublished - Aug 2018

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Vegetables
Fruit
Biomarkers
Carotenoids
Antioxidants
Ascorbic Acid
DNA Damage
Diet
Lutein
Vitamin B Complex
Vascular Stiffness
Genomic Instability
Phytochemicals
Homocysteine
Genetic Markers
Vascular Diseases
Folic Acid
Vitamins
Blood Vessels
Healthy Volunteers

Keywords

  • fruit
  • vegetables
  • human intervention
  • dietary change
  • biomarkers
  • attitudes

ASJC Scopus subject areas

  • Medicine(all)
  • Nursing(all)

Cite this

Effect of increasing fruit and vegetable intake by dietary intervention on nutritional biomarkers and attitudes to dietary change : a randomised trial. / Duthie, Susan J.; Duthie, Garry G.; Russell, Wendy R.; Kyle, Janet A. M.; Macdiarmid, Jennie I.; Rungapamestry, Vanessa; Stephen, Sylvia; Megias-Baeza, Cristina; Kaniewska, Joanna J.; Shaw, Lindsey; Milne, Lesley; Bremner, David; Ross, Karen; Morrice, Philip; Pirie, Lynn P.; Horgan, Graham; Bestwick, Charles S.

In: European Journal of Nutrition, Vol. 57, No. 5, 08.2018, p. 1855-1872.

Research output: Contribution to journalArticle

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T1 - Effect of increasing fruit and vegetable intake by dietary intervention on nutritional biomarkers and attitudes to dietary change

T2 - a randomised trial

AU - Duthie, Susan J.

AU - Duthie, Garry G.

AU - Russell, Wendy R.

AU - Kyle, Janet A. M.

AU - Macdiarmid, Jennie I.

AU - Rungapamestry, Vanessa

AU - Stephen, Sylvia

AU - Megias-Baeza, Cristina

AU - Kaniewska, Joanna J.

AU - Shaw, Lindsey

AU - Milne, Lesley

AU - Bremner, David

AU - Ross, Karen

AU - Morrice, Philip

AU - Pirie, Lynn P.

AU - Horgan, Graham

AU - Bestwick, Charles S.

N1 - This work was funded by The Scottish Government Rural and Environmental Science and Analytical Sciences Division (RESAS) and supported by the Rank Prize Funds.

PY - 2018/8

Y1 - 2018/8

N2 - PURPOSE: Low fruit and vegetable consumption is linked with an increased risk of death from vascular disease and cancer. The benefit of eating fruits and vegetables is attributed in part to antioxidants, vitamins and phytochemicals. Whether increasing intake impacts on markers of disease remains to be established. This study investigates whether increasing daily intake of fruits, vegetables and juices from low (approx. 3 portions), to high intakes (approx. 8 portions) impacts on nutritional and clinical biomarkers. Barriers to achieving the recommended fruit and vegetable intakes are also investigated.METHOD: In a randomised clinical trial, the participants [19 men and 26 women (39-58 years)] with low reported fruit, juice and vegetable intake (<3 portions/day) were randomised to consume either their usual diet or a diet supplemented with an additional 480 g of fruit and vegetables and fruit juice (300 ml) daily for 12 weeks. Nutritional biomarkers (vitamin C, carotenoids, B vitamins), antioxidant capacity and genomic stability were measured pre-intervention, at 4-, 8- and 12 weeks throughout the intervention. Samples were also taken post-intervention after a 6-week washout period. Glucose, homocysteine, lipids, blood pressure, weight and arterial stiffness were also measured. Intake of fruit, fruit juice and vegetables was reassessed 12 months after conducting the study and a questionnaire was developed to identify barriers to healthy eating.RESULTS: Intake increased significantly in the intervention group compared to controls, achieving 8.4 portions/day after 12 weeks. Plasma vitamin C (35%), folate (15%) and certain carotenoids [α-carotene (50%) and β-carotene (70%) and lutein/zeaxanthin (70%)] were significantly increased (P < 0.05) in the intervention group. There were no significant changes in antioxidant capacity, DNA damage and markers of vascular health. Barriers to achieving recommended intakes of fruits and vegetables measured 12 months after the intervention period were amount, inconvenience and cost.CONCLUSION: While increasing fruit, juice and vegetable consumption increases circulating level of beneficial nutrients in healthy subjects, a 12-week intervention was not associated with effects on antioxidant status or lymphocyte DNA damage.TRIAL REGISTRATION: This trial was registered at Controlled-Trials.com; registration ISRCTN71368072.

AB - PURPOSE: Low fruit and vegetable consumption is linked with an increased risk of death from vascular disease and cancer. The benefit of eating fruits and vegetables is attributed in part to antioxidants, vitamins and phytochemicals. Whether increasing intake impacts on markers of disease remains to be established. This study investigates whether increasing daily intake of fruits, vegetables and juices from low (approx. 3 portions), to high intakes (approx. 8 portions) impacts on nutritional and clinical biomarkers. Barriers to achieving the recommended fruit and vegetable intakes are also investigated.METHOD: In a randomised clinical trial, the participants [19 men and 26 women (39-58 years)] with low reported fruit, juice and vegetable intake (<3 portions/day) were randomised to consume either their usual diet or a diet supplemented with an additional 480 g of fruit and vegetables and fruit juice (300 ml) daily for 12 weeks. Nutritional biomarkers (vitamin C, carotenoids, B vitamins), antioxidant capacity and genomic stability were measured pre-intervention, at 4-, 8- and 12 weeks throughout the intervention. Samples were also taken post-intervention after a 6-week washout period. Glucose, homocysteine, lipids, blood pressure, weight and arterial stiffness were also measured. Intake of fruit, fruit juice and vegetables was reassessed 12 months after conducting the study and a questionnaire was developed to identify barriers to healthy eating.RESULTS: Intake increased significantly in the intervention group compared to controls, achieving 8.4 portions/day after 12 weeks. Plasma vitamin C (35%), folate (15%) and certain carotenoids [α-carotene (50%) and β-carotene (70%) and lutein/zeaxanthin (70%)] were significantly increased (P < 0.05) in the intervention group. There were no significant changes in antioxidant capacity, DNA damage and markers of vascular health. Barriers to achieving recommended intakes of fruits and vegetables measured 12 months after the intervention period were amount, inconvenience and cost.CONCLUSION: While increasing fruit, juice and vegetable consumption increases circulating level of beneficial nutrients in healthy subjects, a 12-week intervention was not associated with effects on antioxidant status or lymphocyte DNA damage.TRIAL REGISTRATION: This trial was registered at Controlled-Trials.com; registration ISRCTN71368072.

KW - fruit

KW - vegetables

KW - human intervention

KW - dietary change

KW - biomarkers

KW - attitudes

U2 - 10.1007/s00394-017-1469-0

DO - 10.1007/s00394-017-1469-0

M3 - Article

VL - 57

SP - 1855

EP - 1872

JO - European Journal of Nutrition

JF - European Journal of Nutrition

SN - 1436-6207

IS - 5

ER -