Effect of interleukin-8 (IL-8), anti-IL-8, and IL-12 on endometrial cell survival in combined endometrial gland and stromal cell cultures derived from women with and without endometriosis

R. Gazvani, Lorna Margaret Smith, Paul Alfred Francois Fowler

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Objective: To study the affects of interleukin-8 (IL-8), anti-IL-8, and IL-12 on in vitro proliferation of endometrial cells.

Design: An in vitro study.

Setting: Department of Obstetrics and Gynecology, University of Aberdeen, UK.

Patient(s): Women attending a fertility clinic.

Intervention(s): In vitro cell cultures using culture media supplemented with IL-8 (100 ng/mL, 200 ng/mL, and 500 ng/mL), IL-12 (1 ng/mL, 5 ng/mL, and 25 ng/mL), and anti-IL-8 (0.1 mug/mL, 1 mug/mL, 10 mug/mL).

Main Outcome Measure(s): In vitro survival of dispersed endometrial cells (combined epithelial and glandular) at 5 and 9 days of culture.

Result(s): There was a dose-dependent stimulatory effect of IL-8 on survival of cells. From women with and without endometriosis. IL-12 at 1 ng/mL significantly inhibited the survival of endometrial cells from women without endometriosis as compared with cells from women with endometriosis. At 1 mug/mL, anti-IL-8 significantly inhibited the survival of endometrial cells from women with endometriosis compared with cells from women without endometriosis on day 5 of culture.

Conclusion(s): Our findings confirm the stimulatory effects of IL-8 and its possible role in the pathogenesis of endometriosis. The effects of IL-12 and anti-IL-8 on endometrial cell survival varied according to the disease state and the concentration of the cytokines. Future in vitro studies on the role of anti-IL-8 and IL-12 should aim to use a greater range of concentrations and a higher density of endometrial cells in cultures supplemented with monocytes. (Fertil Steril(R) 2002;77:62-7. (C) 2002 by American Society for Reproductive Medicine.).

Original languageEnglish
Pages (from-to)62-67
Number of pages5
JournalFertility and Sterility
Volume77
Issue number1
Early online date30 Dec 2001
DOIs
Publication statusPublished - Jan 2002

Keywords

  • interleukin-8
  • anti-interleukin-8
  • interleukin-12
  • endometriosis
  • cell culture
  • endothelial growth-factor
  • peritoneal-fluid
  • angiogenesis
  • cytokines
  • expression
  • disease
  • model

Cite this

@article{8d170ce570854e0ea52baec31d24157e,
title = "Effect of interleukin-8 (IL-8), anti-IL-8, and IL-12 on endometrial cell survival in combined endometrial gland and stromal cell cultures derived from women with and without endometriosis",
abstract = "Objective: To study the affects of interleukin-8 (IL-8), anti-IL-8, and IL-12 on in vitro proliferation of endometrial cells.Design: An in vitro study.Setting: Department of Obstetrics and Gynecology, University of Aberdeen, UK.Patient(s): Women attending a fertility clinic.Intervention(s): In vitro cell cultures using culture media supplemented with IL-8 (100 ng/mL, 200 ng/mL, and 500 ng/mL), IL-12 (1 ng/mL, 5 ng/mL, and 25 ng/mL), and anti-IL-8 (0.1 mug/mL, 1 mug/mL, 10 mug/mL).Main Outcome Measure(s): In vitro survival of dispersed endometrial cells (combined epithelial and glandular) at 5 and 9 days of culture.Result(s): There was a dose-dependent stimulatory effect of IL-8 on survival of cells. From women with and without endometriosis. IL-12 at 1 ng/mL significantly inhibited the survival of endometrial cells from women without endometriosis as compared with cells from women with endometriosis. At 1 mug/mL, anti-IL-8 significantly inhibited the survival of endometrial cells from women with endometriosis compared with cells from women without endometriosis on day 5 of culture.Conclusion(s): Our findings confirm the stimulatory effects of IL-8 and its possible role in the pathogenesis of endometriosis. The effects of IL-12 and anti-IL-8 on endometrial cell survival varied according to the disease state and the concentration of the cytokines. Future in vitro studies on the role of anti-IL-8 and IL-12 should aim to use a greater range of concentrations and a higher density of endometrial cells in cultures supplemented with monocytes. (Fertil Steril(R) 2002;77:62-7. (C) 2002 by American Society for Reproductive Medicine.).",
keywords = "interleukin-8, anti-interleukin-8, interleukin-12, endometriosis, cell culture, endothelial growth-factor, peritoneal-fluid, angiogenesis, cytokines, expression, disease, model",
author = "R. Gazvani and Smith, {Lorna Margaret} and Fowler, {Paul Alfred Francois}",
year = "2002",
month = "1",
doi = "10.1016/S0015-0282(01)02954-5",
language = "English",
volume = "77",
pages = "62--67",
journal = "Fertility and Sterility",
issn = "0015-0282",
publisher = "ACADEMIC PRESS INC ELSEVIER SCIENCE",
number = "1",

}

TY - JOUR

T1 - Effect of interleukin-8 (IL-8), anti-IL-8, and IL-12 on endometrial cell survival in combined endometrial gland and stromal cell cultures derived from women with and without endometriosis

AU - Gazvani, R.

AU - Smith, Lorna Margaret

AU - Fowler, Paul Alfred Francois

PY - 2002/1

Y1 - 2002/1

N2 - Objective: To study the affects of interleukin-8 (IL-8), anti-IL-8, and IL-12 on in vitro proliferation of endometrial cells.Design: An in vitro study.Setting: Department of Obstetrics and Gynecology, University of Aberdeen, UK.Patient(s): Women attending a fertility clinic.Intervention(s): In vitro cell cultures using culture media supplemented with IL-8 (100 ng/mL, 200 ng/mL, and 500 ng/mL), IL-12 (1 ng/mL, 5 ng/mL, and 25 ng/mL), and anti-IL-8 (0.1 mug/mL, 1 mug/mL, 10 mug/mL).Main Outcome Measure(s): In vitro survival of dispersed endometrial cells (combined epithelial and glandular) at 5 and 9 days of culture.Result(s): There was a dose-dependent stimulatory effect of IL-8 on survival of cells. From women with and without endometriosis. IL-12 at 1 ng/mL significantly inhibited the survival of endometrial cells from women without endometriosis as compared with cells from women with endometriosis. At 1 mug/mL, anti-IL-8 significantly inhibited the survival of endometrial cells from women with endometriosis compared with cells from women without endometriosis on day 5 of culture.Conclusion(s): Our findings confirm the stimulatory effects of IL-8 and its possible role in the pathogenesis of endometriosis. The effects of IL-12 and anti-IL-8 on endometrial cell survival varied according to the disease state and the concentration of the cytokines. Future in vitro studies on the role of anti-IL-8 and IL-12 should aim to use a greater range of concentrations and a higher density of endometrial cells in cultures supplemented with monocytes. (Fertil Steril(R) 2002;77:62-7. (C) 2002 by American Society for Reproductive Medicine.).

AB - Objective: To study the affects of interleukin-8 (IL-8), anti-IL-8, and IL-12 on in vitro proliferation of endometrial cells.Design: An in vitro study.Setting: Department of Obstetrics and Gynecology, University of Aberdeen, UK.Patient(s): Women attending a fertility clinic.Intervention(s): In vitro cell cultures using culture media supplemented with IL-8 (100 ng/mL, 200 ng/mL, and 500 ng/mL), IL-12 (1 ng/mL, 5 ng/mL, and 25 ng/mL), and anti-IL-8 (0.1 mug/mL, 1 mug/mL, 10 mug/mL).Main Outcome Measure(s): In vitro survival of dispersed endometrial cells (combined epithelial and glandular) at 5 and 9 days of culture.Result(s): There was a dose-dependent stimulatory effect of IL-8 on survival of cells. From women with and without endometriosis. IL-12 at 1 ng/mL significantly inhibited the survival of endometrial cells from women without endometriosis as compared with cells from women with endometriosis. At 1 mug/mL, anti-IL-8 significantly inhibited the survival of endometrial cells from women with endometriosis compared with cells from women without endometriosis on day 5 of culture.Conclusion(s): Our findings confirm the stimulatory effects of IL-8 and its possible role in the pathogenesis of endometriosis. The effects of IL-12 and anti-IL-8 on endometrial cell survival varied according to the disease state and the concentration of the cytokines. Future in vitro studies on the role of anti-IL-8 and IL-12 should aim to use a greater range of concentrations and a higher density of endometrial cells in cultures supplemented with monocytes. (Fertil Steril(R) 2002;77:62-7. (C) 2002 by American Society for Reproductive Medicine.).

KW - interleukin-8

KW - anti-interleukin-8

KW - interleukin-12

KW - endometriosis

KW - cell culture

KW - endothelial growth-factor

KW - peritoneal-fluid

KW - angiogenesis

KW - cytokines

KW - expression

KW - disease

KW - model

U2 - 10.1016/S0015-0282(01)02954-5

DO - 10.1016/S0015-0282(01)02954-5

M3 - Article

VL - 77

SP - 62

EP - 67

JO - Fertility and Sterility

JF - Fertility and Sterility

SN - 0015-0282

IS - 1

ER -