Effect of interleukin-8 (IL-8), anti-IL-8, and IL-12 on endometrial cell survival in combined endometrial gland and stromal cell cultures derived from women with and without endometriosis

Objective: To study the affects of interleukin-8 (IL-8), anti-IL-8, and IL-12 on in vitro proliferation of endometrial cells.

Design: An in vitro study.

Setting: Department of Obstetrics and Gynecology, University of Aberdeen, UK.

Patient(s): Women attending a fertility clinic.

Intervention(s): In vitro cell cultures using culture media supplemented with IL-8 (100 ng/mL, 200 ng/mL, and 500 ng/mL), IL-12 (1 ng/mL, 5 ng/mL, and 25 ng/mL), and anti-IL-8 (0.1 mug/mL, 1 mug/mL, 10 mug/mL).

Main Outcome Measure(s): In vitro survival of dispersed endometrial cells (combined epithelial and glandular) at 5 and 9 days of culture.

Result(s): There was a dose-dependent stimulatory effect of IL-8 on survival of cells. From women with and without endometriosis. IL-12 at 1 ng/mL significantly inhibited the survival of endometrial cells from women without endometriosis as compared with cells from women with endometriosis. At 1 mug/mL, anti-IL-8 significantly inhibited the survival of endometrial cells from women with endometriosis compared with cells from women without endometriosis on day 5 of culture.

Conclusion(s): Our findings confirm the stimulatory effects of IL-8 and its possible role in the pathogenesis of endometriosis. The effects of IL-12 and anti-IL-8 on endometrial cell survival varied according to the disease state and the concentration of the cytokines. Future in vitro studies on the role of anti-IL-8 and IL-12 should aim to use a greater range of concentrations and a higher density of endometrial cells in cultures supplemented with monocytes. (Fertil Steril(R) 2002;77:62-7. (C) 2002 by American Society for Reproductive Medicine.).