Effect of intraperitoneally administered IL-1b-derived peptides on resistance to viral haemorrhagic septicaemia in rainbow trout Oncorhynchus mykiss

Christopher John Secombes

Research output: Contribution to journalArticle

Abstract

The present work provides the first information concerning the immunostimulatory activity of trout interleukin (IL)-1beta-derived peptides in vivo. Previous studies have demonstrated the ability of 2 such peptides, referred to as P1 and P3, to up-regulate a range of important immune parameters in vitro, P1 corresponds to fragment 146-157 (YVTPVPIETEAR) of the trout sequence and is analogous to a biologically active mammalian IL-1beta-derived peptide, whilst P3 was synthesised to complex with the IL-1 receptor and corresponds to fragment 197-206 (YRRNTGVDIS) of the trout sequence. Optimal migration of peritoneal leucocytes, peptide induced phagocytosis and intracellular respiratory burst activity occurred following intraperitoneal injection of 3.0 mumol of P3. Furthermore, resistance to viral haemorrhagic septicaemia virus (VHSV) was soon augmented (2 d) post-injection of P3.

Original languageEnglish
Pages (from-to)195-200
Number of pages5
JournalDiseases of Aquatic Organisms
Volume56
Publication statusPublished - 2003

Keywords

  • interleukin-beta
  • phagocytosis
  • respiratory burst
  • disease resistance
  • immunomodulation
  • VHS
  • NITRIC-OXIDE SYNTHASE
  • NECROSIS-FACTOR-ALPHA
  • LEUKOCYTES IN-VITRO
  • RECOMBINANT IL-1-BETA
  • MICROSPHERES
  • EXPRESSION
  • PHAGOCYTOSIS
  • ORGANIZATION
  • ENHANCEMENT
  • CLONING

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