Effect of long-term cold exposure on antioxidant enzyme activities in a small mammal

C Selman, J S McLaren, M J Himanka, J R Speakman

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

Aerobic organisms continually face exposure to reactive oxygen species (ROS) and many have evolved sophisticated antioxidant systems to effectively remove them. Any increase in ROS production or weakening in this defense system may ultimately lead to oxidative stress and cellular damage. We investigated whether long-term cold exposure, which is known to lead to an elevation in metabolic rate, increased the activities of the ROS-scavenging enzymes, catalase (CAT), selenium-dependent glutathione peroxidase (GPx), and total superoxide dismutase (Total-SOD) in liver, cardiac muscle, kidney, skeletal muscle (vastus lateralis), and duodenum of shea-tailed field voles (Microtus agrestis), born and maintained at either 8 +/- 3 degrees C or 22 +/- 3 degrees C. CAT, GPx, and Total-SOD activities were determined at age 61 +/- 1.9 days. An increase in CAT activity in voles maintained at 8 +/- 3 degrees C was observed in skeletal muscle (71%) and kidney (20%), with both CAT and GPx activities significantly elevated (by 40 and 43%, respectively) in cardiac muscle, when compared to voles at 22 +/- 3 degrees C. Total-SOD activity and protein content did not differ significantly between groups in any tissue. We suggest that the compensatory increases in CAT (skeletal muscle, cardiac muscle, kidney) and GPx (cardiac muscle), but not Total-SOD activities, resulting from long-term. cold exposure may reflect the elevated metabolic rate, and possibly also increased ROS production, at this time. (C) 2000 Elsevier Science Inc.

Original languageEnglish
Pages (from-to)1279-1285
Number of pages7
JournalFree Radical Biology and Medicine
Volume28
Publication statusPublished - 2000

Keywords

  • cold acclimation
  • free radicals
  • catalase
  • glutathione peroxidase
  • superoxide dismutase
  • short-tailed field vole
  • Microtus agrestis
  • SUSTAINABLE METABOLIC-RATE
  • OXIDATIVE STRESS
  • LIPID-PEROXIDATION
  • LIFE-SPAN
  • SUPEROXIDE-DISMUTASE
  • GLUTATHIONE SYSTEM
  • MICROTUS-AGRESTIS
  • EXERCISE
  • LONGEVITY
  • ACCLIMATION

Cite this

Effect of long-term cold exposure on antioxidant enzyme activities in a small mammal. / Selman, C ; McLaren, J S ; Himanka, M J ; Speakman, J R .

In: Free Radical Biology and Medicine, Vol. 28, 2000, p. 1279-1285.

Research output: Contribution to journalArticle

@article{3b4837360a7f411ca96f11e843982ad7,
title = "Effect of long-term cold exposure on antioxidant enzyme activities in a small mammal",
abstract = "Aerobic organisms continually face exposure to reactive oxygen species (ROS) and many have evolved sophisticated antioxidant systems to effectively remove them. Any increase in ROS production or weakening in this defense system may ultimately lead to oxidative stress and cellular damage. We investigated whether long-term cold exposure, which is known to lead to an elevation in metabolic rate, increased the activities of the ROS-scavenging enzymes, catalase (CAT), selenium-dependent glutathione peroxidase (GPx), and total superoxide dismutase (Total-SOD) in liver, cardiac muscle, kidney, skeletal muscle (vastus lateralis), and duodenum of shea-tailed field voles (Microtus agrestis), born and maintained at either 8 +/- 3 degrees C or 22 +/- 3 degrees C. CAT, GPx, and Total-SOD activities were determined at age 61 +/- 1.9 days. An increase in CAT activity in voles maintained at 8 +/- 3 degrees C was observed in skeletal muscle (71{\%}) and kidney (20{\%}), with both CAT and GPx activities significantly elevated (by 40 and 43{\%}, respectively) in cardiac muscle, when compared to voles at 22 +/- 3 degrees C. Total-SOD activity and protein content did not differ significantly between groups in any tissue. We suggest that the compensatory increases in CAT (skeletal muscle, cardiac muscle, kidney) and GPx (cardiac muscle), but not Total-SOD activities, resulting from long-term. cold exposure may reflect the elevated metabolic rate, and possibly also increased ROS production, at this time. (C) 2000 Elsevier Science Inc.",
keywords = "cold acclimation, free radicals, catalase, glutathione peroxidase, superoxide dismutase, short-tailed field vole, Microtus agrestis, SUSTAINABLE METABOLIC-RATE, OXIDATIVE STRESS, LIPID-PEROXIDATION, LIFE-SPAN, SUPEROXIDE-DISMUTASE, GLUTATHIONE SYSTEM, MICROTUS-AGRESTIS, EXERCISE, LONGEVITY, ACCLIMATION",
author = "C Selman and McLaren, {J S} and Himanka, {M J} and Speakman, {J R}",
year = "2000",
language = "English",
volume = "28",
pages = "1279--1285",
journal = "Free Radical Biology and Medicine",
issn = "0891-5849",
publisher = "Elsevier Inc.",

}

TY - JOUR

T1 - Effect of long-term cold exposure on antioxidant enzyme activities in a small mammal

AU - Selman, C

AU - McLaren, J S

AU - Himanka, M J

AU - Speakman, J R

PY - 2000

Y1 - 2000

N2 - Aerobic organisms continually face exposure to reactive oxygen species (ROS) and many have evolved sophisticated antioxidant systems to effectively remove them. Any increase in ROS production or weakening in this defense system may ultimately lead to oxidative stress and cellular damage. We investigated whether long-term cold exposure, which is known to lead to an elevation in metabolic rate, increased the activities of the ROS-scavenging enzymes, catalase (CAT), selenium-dependent glutathione peroxidase (GPx), and total superoxide dismutase (Total-SOD) in liver, cardiac muscle, kidney, skeletal muscle (vastus lateralis), and duodenum of shea-tailed field voles (Microtus agrestis), born and maintained at either 8 +/- 3 degrees C or 22 +/- 3 degrees C. CAT, GPx, and Total-SOD activities were determined at age 61 +/- 1.9 days. An increase in CAT activity in voles maintained at 8 +/- 3 degrees C was observed in skeletal muscle (71%) and kidney (20%), with both CAT and GPx activities significantly elevated (by 40 and 43%, respectively) in cardiac muscle, when compared to voles at 22 +/- 3 degrees C. Total-SOD activity and protein content did not differ significantly between groups in any tissue. We suggest that the compensatory increases in CAT (skeletal muscle, cardiac muscle, kidney) and GPx (cardiac muscle), but not Total-SOD activities, resulting from long-term. cold exposure may reflect the elevated metabolic rate, and possibly also increased ROS production, at this time. (C) 2000 Elsevier Science Inc.

AB - Aerobic organisms continually face exposure to reactive oxygen species (ROS) and many have evolved sophisticated antioxidant systems to effectively remove them. Any increase in ROS production or weakening in this defense system may ultimately lead to oxidative stress and cellular damage. We investigated whether long-term cold exposure, which is known to lead to an elevation in metabolic rate, increased the activities of the ROS-scavenging enzymes, catalase (CAT), selenium-dependent glutathione peroxidase (GPx), and total superoxide dismutase (Total-SOD) in liver, cardiac muscle, kidney, skeletal muscle (vastus lateralis), and duodenum of shea-tailed field voles (Microtus agrestis), born and maintained at either 8 +/- 3 degrees C or 22 +/- 3 degrees C. CAT, GPx, and Total-SOD activities were determined at age 61 +/- 1.9 days. An increase in CAT activity in voles maintained at 8 +/- 3 degrees C was observed in skeletal muscle (71%) and kidney (20%), with both CAT and GPx activities significantly elevated (by 40 and 43%, respectively) in cardiac muscle, when compared to voles at 22 +/- 3 degrees C. Total-SOD activity and protein content did not differ significantly between groups in any tissue. We suggest that the compensatory increases in CAT (skeletal muscle, cardiac muscle, kidney) and GPx (cardiac muscle), but not Total-SOD activities, resulting from long-term. cold exposure may reflect the elevated metabolic rate, and possibly also increased ROS production, at this time. (C) 2000 Elsevier Science Inc.

KW - cold acclimation

KW - free radicals

KW - catalase

KW - glutathione peroxidase

KW - superoxide dismutase

KW - short-tailed field vole

KW - Microtus agrestis

KW - SUSTAINABLE METABOLIC-RATE

KW - OXIDATIVE STRESS

KW - LIPID-PEROXIDATION

KW - LIFE-SPAN

KW - SUPEROXIDE-DISMUTASE

KW - GLUTATHIONE SYSTEM

KW - MICROTUS-AGRESTIS

KW - EXERCISE

KW - LONGEVITY

KW - ACCLIMATION

M3 - Article

VL - 28

SP - 1279

EP - 1285

JO - Free Radical Biology and Medicine

JF - Free Radical Biology and Medicine

SN - 0891-5849

ER -