Effect of maternal polyunsaturated fatty acid concentration on transport by the human placenta

Paul Haggarty, J Ashton, M Joynson, D R Abramovich, K Page

    Research output: Contribution to journalArticle

    73 Citations (Scopus)

    Abstract

    The role of the placenta in controlling the supply of fatty acids to the fetus was investigated in term placentas (n = 5) from normal pregnancies. The maternal side was perfused ex vivo for 90 min with a modified Krebs Ringer solution containing a physiological mixture of fatty acids - designed to mimic the composition of non-esterified fatty acids (NEFA) measured in the last trimester of pregnancy (n = 10) - and ratio of fatty acid to human albumin. The selectivity for cr-linolenic acid (alpha LN) transfer to the fetal circulation was not significantly different from that observed when using the triglyceride (TG) composition (1.21 +/- 0.04), but significantly different for AA (1.43 +/- 0.12; p < 0.001) and docosahexaenoic acid (DHA; 2.02 +/- 0.09; p = 0.048). The absolute rate of transfer (nmol.ml(-1)) compared to that using the TG maternal perfusate composition was significantly different for LA (0.562 +/- 0.038; p 0.50), alpha LN (0.130 +/- 0.009; p < 0.001), arachidonic acid (AA; 0.218 +/- 0.022; p = 0.001) and DHA (0.383 +/- 0.04; p < 0.001). Thus, placental selectivity for aLN and DHA appears to be relatively unresponsive to changes in the mixture of fatty acids in the maternal circulation but the selectivity for AA increased with the increase in the maternal AA concentration. For an 8-fold increase in the concentration of DHA in the maternal circulation there was a 13-fold increase in the transfer of DHA to the fetal circulation. For a 2-fold increase in the concentration of AA, transfer was increased 8-fold. For a 1.3-fold increase in the concentration of alpha LN, transfer was increased 2.1-fold. These results suggest that the maternal concentration of individual fatty acids, and hence the composition of the maternal diet, can have large effects on polyunsaturated/long-chain polyunsaturated fatty acids delivery to the fetus.

    Original languageEnglish
    Pages (from-to)350-359
    Number of pages10
    JournalBiology of the Neonate
    Volume75
    Issue number6
    Publication statusPublished - Jun 1999

    Keywords

    • placenta
    • transport
    • polyunsaturated fatty acids
    • docosahexaenoic acid
    • n-3
    • n-6
    • pregnancy
    • DOCOSAHEXAENOIC ACID
    • OIL SUPPLEMENTATION
    • PRETERM INFANTS
    • LATE PREGNANCY
    • HUMAN-MILK
    • FORMULA
    • ACUITY
    • GROWTH
    • BRAIN

    Cite this

    Effect of maternal polyunsaturated fatty acid concentration on transport by the human placenta. / Haggarty, Paul; Ashton, J ; Joynson, M ; Abramovich, D R ; Page, K .

    In: Biology of the Neonate, Vol. 75, No. 6, 06.1999, p. 350-359.

    Research output: Contribution to journalArticle

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    T1 - Effect of maternal polyunsaturated fatty acid concentration on transport by the human placenta

    AU - Haggarty, Paul

    AU - Ashton, J

    AU - Joynson, M

    AU - Abramovich, D R

    AU - Page, K

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    N2 - The role of the placenta in controlling the supply of fatty acids to the fetus was investigated in term placentas (n = 5) from normal pregnancies. The maternal side was perfused ex vivo for 90 min with a modified Krebs Ringer solution containing a physiological mixture of fatty acids - designed to mimic the composition of non-esterified fatty acids (NEFA) measured in the last trimester of pregnancy (n = 10) - and ratio of fatty acid to human albumin. The selectivity for cr-linolenic acid (alpha LN) transfer to the fetal circulation was not significantly different from that observed when using the triglyceride (TG) composition (1.21 +/- 0.04), but significantly different for AA (1.43 +/- 0.12; p < 0.001) and docosahexaenoic acid (DHA; 2.02 +/- 0.09; p = 0.048). The absolute rate of transfer (nmol.ml(-1)) compared to that using the TG maternal perfusate composition was significantly different for LA (0.562 +/- 0.038; p 0.50), alpha LN (0.130 +/- 0.009; p < 0.001), arachidonic acid (AA; 0.218 +/- 0.022; p = 0.001) and DHA (0.383 +/- 0.04; p < 0.001). Thus, placental selectivity for aLN and DHA appears to be relatively unresponsive to changes in the mixture of fatty acids in the maternal circulation but the selectivity for AA increased with the increase in the maternal AA concentration. For an 8-fold increase in the concentration of DHA in the maternal circulation there was a 13-fold increase in the transfer of DHA to the fetal circulation. For a 2-fold increase in the concentration of AA, transfer was increased 8-fold. For a 1.3-fold increase in the concentration of alpha LN, transfer was increased 2.1-fold. These results suggest that the maternal concentration of individual fatty acids, and hence the composition of the maternal diet, can have large effects on polyunsaturated/long-chain polyunsaturated fatty acids delivery to the fetus.

    AB - The role of the placenta in controlling the supply of fatty acids to the fetus was investigated in term placentas (n = 5) from normal pregnancies. The maternal side was perfused ex vivo for 90 min with a modified Krebs Ringer solution containing a physiological mixture of fatty acids - designed to mimic the composition of non-esterified fatty acids (NEFA) measured in the last trimester of pregnancy (n = 10) - and ratio of fatty acid to human albumin. The selectivity for cr-linolenic acid (alpha LN) transfer to the fetal circulation was not significantly different from that observed when using the triglyceride (TG) composition (1.21 +/- 0.04), but significantly different for AA (1.43 +/- 0.12; p < 0.001) and docosahexaenoic acid (DHA; 2.02 +/- 0.09; p = 0.048). The absolute rate of transfer (nmol.ml(-1)) compared to that using the TG maternal perfusate composition was significantly different for LA (0.562 +/- 0.038; p 0.50), alpha LN (0.130 +/- 0.009; p < 0.001), arachidonic acid (AA; 0.218 +/- 0.022; p = 0.001) and DHA (0.383 +/- 0.04; p < 0.001). Thus, placental selectivity for aLN and DHA appears to be relatively unresponsive to changes in the mixture of fatty acids in the maternal circulation but the selectivity for AA increased with the increase in the maternal AA concentration. For an 8-fold increase in the concentration of DHA in the maternal circulation there was a 13-fold increase in the transfer of DHA to the fetal circulation. For a 2-fold increase in the concentration of AA, transfer was increased 8-fold. For a 1.3-fold increase in the concentration of alpha LN, transfer was increased 2.1-fold. These results suggest that the maternal concentration of individual fatty acids, and hence the composition of the maternal diet, can have large effects on polyunsaturated/long-chain polyunsaturated fatty acids delivery to the fetus.

    KW - placenta

    KW - transport

    KW - polyunsaturated fatty acids

    KW - docosahexaenoic acid

    KW - n-3

    KW - n-6

    KW - pregnancy

    KW - DOCOSAHEXAENOIC ACID

    KW - OIL SUPPLEMENTATION

    KW - PRETERM INFANTS

    KW - LATE PREGNANCY

    KW - HUMAN-MILK

    KW - FORMULA

    KW - ACUITY

    KW - GROWTH

    KW - BRAIN

    M3 - Article

    VL - 75

    SP - 350

    EP - 359

    JO - Biology of the Neonate

    JF - Biology of the Neonate

    SN - 0006-3126

    IS - 6

    ER -