Effect of maternal undernutrition during pregnancy on early ovarian development and subsequent follicular development in sheep fetuses

M. T. Rae, S. Palassio, C.E. Kyle, A. N. Brooks, R. G. Lea, David Warren Miller, S. M. Rhind

    Research output: Contribution to journalArticle

    103 Citations (Scopus)

    Abstract

    Gonad development in female sheep fetuses is thought to occur in a number of key stages. The aim of this study was to determine the effects of maternal undernutrition, applied at one or more of these critical stages, on fetal ovarian development. Groups of ewes (n = 11-19) were fed rations providing either 100% (high; H) or 50% (low; L) of energy requirements for live weight maintenance during selected 'windows' during gestation. Control ewes (HH and HHH) were fed the H ration from mating until they were killed at days 50, 65 (HH) or 110 (HHH) of gestation, whereas ewes of other groups were fed the L ration for the periods between day 0 and day 30 of gestation (LH and LHH), day 31 and day 50 or 65 of gestation (H L and H LH), day 65 and day 110 of gestation (HHL) or day 0 of gestation until the animals were killed (LL and LLL). At day 50 of gestation, there was no effect of nutritional treatment on mean fetal mass but compared with HH animals, mean fetal ovarian mass was significantly lower in HL (P < 0.05) and LL (P < 0.001) animals. At day 65 of gestation, there were significantly fewer germ cells (P < 0.05) at the resting, diplotene stage of initial meiosis in LL animals than there were in HH animals, indicating delayed germ cell maturation and onset of meiosis. Qualitative assessment of proliferative cell nuclear antigen immunostaining indicated that, at day 50 of gestation, staining was located predominantly in the germ cells, whereas by day 65 of gestation, staining was confined predominantly to somatic cells. Undernutrition in each one of these windows was associated with delayed ovarian follicular development (P < 0.05-0.001) as measured by development of the granulosa cell layer at day 110 of gestation. This study demonstrates that undernutrition before and during folliculogenesis can delay fetal follicular development.

    Original languageEnglish
    Pages (from-to)915-922
    Number of pages7
    JournalReproduction
    Volume122
    DOIs
    Publication statusPublished - 2001

    Keywords

    • SCOTTISH BLACKFACE EWES
    • REPRODUCTIVE-PERFORMANCE
    • GROWTH
    • PITUITARY

    Cite this

    Rae, M. T., Palassio, S., Kyle, C. E., Brooks, A. N., Lea, R. G., Miller, D. W., & Rhind, S. M. (2001). Effect of maternal undernutrition during pregnancy on early ovarian development and subsequent follicular development in sheep fetuses. Reproduction, 122, 915-922. https://doi.org/10.1530/rep.0.1220915

    Effect of maternal undernutrition during pregnancy on early ovarian development and subsequent follicular development in sheep fetuses. / Rae, M. T.; Palassio, S.; Kyle, C.E.; Brooks, A. N.; Lea, R. G.; Miller, David Warren; Rhind, S. M.

    In: Reproduction, Vol. 122, 2001, p. 915-922.

    Research output: Contribution to journalArticle

    Rae, M. T. ; Palassio, S. ; Kyle, C.E. ; Brooks, A. N. ; Lea, R. G. ; Miller, David Warren ; Rhind, S. M. / Effect of maternal undernutrition during pregnancy on early ovarian development and subsequent follicular development in sheep fetuses. In: Reproduction. 2001 ; Vol. 122. pp. 915-922.
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    T1 - Effect of maternal undernutrition during pregnancy on early ovarian development and subsequent follicular development in sheep fetuses

    AU - Rae, M. T.

    AU - Palassio, S.

    AU - Kyle, C.E.

    AU - Brooks, A. N.

    AU - Lea, R. G.

    AU - Miller, David Warren

    AU - Rhind, S. M.

    PY - 2001

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    N2 - Gonad development in female sheep fetuses is thought to occur in a number of key stages. The aim of this study was to determine the effects of maternal undernutrition, applied at one or more of these critical stages, on fetal ovarian development. Groups of ewes (n = 11-19) were fed rations providing either 100% (high; H) or 50% (low; L) of energy requirements for live weight maintenance during selected 'windows' during gestation. Control ewes (HH and HHH) were fed the H ration from mating until they were killed at days 50, 65 (HH) or 110 (HHH) of gestation, whereas ewes of other groups were fed the L ration for the periods between day 0 and day 30 of gestation (LH and LHH), day 31 and day 50 or 65 of gestation (H L and H LH), day 65 and day 110 of gestation (HHL) or day 0 of gestation until the animals were killed (LL and LLL). At day 50 of gestation, there was no effect of nutritional treatment on mean fetal mass but compared with HH animals, mean fetal ovarian mass was significantly lower in HL (P < 0.05) and LL (P < 0.001) animals. At day 65 of gestation, there were significantly fewer germ cells (P < 0.05) at the resting, diplotene stage of initial meiosis in LL animals than there were in HH animals, indicating delayed germ cell maturation and onset of meiosis. Qualitative assessment of proliferative cell nuclear antigen immunostaining indicated that, at day 50 of gestation, staining was located predominantly in the germ cells, whereas by day 65 of gestation, staining was confined predominantly to somatic cells. Undernutrition in each one of these windows was associated with delayed ovarian follicular development (P < 0.05-0.001) as measured by development of the granulosa cell layer at day 110 of gestation. This study demonstrates that undernutrition before and during folliculogenesis can delay fetal follicular development.

    AB - Gonad development in female sheep fetuses is thought to occur in a number of key stages. The aim of this study was to determine the effects of maternal undernutrition, applied at one or more of these critical stages, on fetal ovarian development. Groups of ewes (n = 11-19) were fed rations providing either 100% (high; H) or 50% (low; L) of energy requirements for live weight maintenance during selected 'windows' during gestation. Control ewes (HH and HHH) were fed the H ration from mating until they were killed at days 50, 65 (HH) or 110 (HHH) of gestation, whereas ewes of other groups were fed the L ration for the periods between day 0 and day 30 of gestation (LH and LHH), day 31 and day 50 or 65 of gestation (H L and H LH), day 65 and day 110 of gestation (HHL) or day 0 of gestation until the animals were killed (LL and LLL). At day 50 of gestation, there was no effect of nutritional treatment on mean fetal mass but compared with HH animals, mean fetal ovarian mass was significantly lower in HL (P < 0.05) and LL (P < 0.001) animals. At day 65 of gestation, there were significantly fewer germ cells (P < 0.05) at the resting, diplotene stage of initial meiosis in LL animals than there were in HH animals, indicating delayed germ cell maturation and onset of meiosis. Qualitative assessment of proliferative cell nuclear antigen immunostaining indicated that, at day 50 of gestation, staining was located predominantly in the germ cells, whereas by day 65 of gestation, staining was confined predominantly to somatic cells. Undernutrition in each one of these windows was associated with delayed ovarian follicular development (P < 0.05-0.001) as measured by development of the granulosa cell layer at day 110 of gestation. This study demonstrates that undernutrition before and during folliculogenesis can delay fetal follicular development.

    KW - SCOTTISH BLACKFACE EWES

    KW - REPRODUCTIVE-PERFORMANCE

    KW - GROWTH

    KW - PITUITARY

    U2 - 10.1530/rep.0.1220915

    DO - 10.1530/rep.0.1220915

    M3 - Article

    VL - 122

    SP - 915

    EP - 922

    JO - Reproduction

    JF - Reproduction

    SN - 1470-1626

    ER -