Effect of periodic feeding on substrate uptake and storage rates by a pure culture of Thiothrix (CT3 strain)

M. Majone, M. Beccari, Davide Dionisi, C. Levantesi, R. Ramadori, V. Tandoi

Research output: Contribution to journalArticle

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Abstract

A pure culture of Thiothrix strain CT3 has been aerobically cultured under periodic acetate feeding in a Sequencing Batch Reactor (SBR) at volumetric organic load rate of 0.12 gCOD L-1 d-1. Two different culture residence times (12 d or 20 d) were adopted as well as two different feed frequencies (1 and 4 d-1, for each culture residence time), the volumetric organic load rate being the same under all conditions. The transient response of the microorganism to the periodic acetate feed was investigated through batch tests with biomass withdrawn from the SBR, as function of the different SBR operating conditions. In all tested conditions, a quick transient response to the acetate spike was observed with fast increase of acetate uptake rate (ranging from 71 to 247 mgCOD gCOD-1 h-1). This transient response was mainly due to acetate storage in form of poly-hydroxybutyrate (ranging from 45% to 64% of the observed yield) whereas the growth response (i.e. increase of production rate of active biomass) generally played a minor role (ranging from 21% to 38% of the observed yield). Apart from this general trend, culture residence time as well as feed frequency had a strong impact on transient behaviour of cultured cells. The overall transient response (i.e. maximum specific substrate removal rate) increased as culture residence time decreased or as feed frequency increased. Moreover, the ratio of storage response and growth response increased as the overall transient response decreased, i.e. the storage response was preferentially maintained when cells presented a lower transient response. The ability of the cells to increase their growth rate with respect to SBR average value was the lowest under the most unfavourable conditions (residence time 20 d, feed frequency 1 d-1) and increased with the increase in maximum substrate uptake rate.
Original languageEnglish
Pages (from-to)177-187
Number of pages11
JournalWater research
Volume41
Issue number1
Early online date27 Oct 2006
DOIs
Publication statusPublished - Jan 2007

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Transient analysis
Batch reactors
residence time
acetate
substrate
Substrates
growth response
Biomass
biomass
Microorganisms
effect
rate
microorganism
Cells
reactor

Keywords

  • Thiothrix CT3
  • PHB storage
  • Transient conditions
  • Sequencing batch reactor

Cite this

Effect of periodic feeding on substrate uptake and storage rates by a pure culture of Thiothrix (CT3 strain). / Majone, M.; Beccari, M.; Dionisi, Davide; Levantesi, C.; Ramadori, R.; Tandoi, V.

In: Water research, Vol. 41, No. 1, 01.2007, p. 177-187.

Research output: Contribution to journalArticle

Majone, M. ; Beccari, M. ; Dionisi, Davide ; Levantesi, C. ; Ramadori, R. ; Tandoi, V. / Effect of periodic feeding on substrate uptake and storage rates by a pure culture of Thiothrix (CT3 strain). In: Water research. 2007 ; Vol. 41, No. 1. pp. 177-187.
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AB - A pure culture of Thiothrix strain CT3 has been aerobically cultured under periodic acetate feeding in a Sequencing Batch Reactor (SBR) at volumetric organic load rate of 0.12 gCOD L-1 d-1. Two different culture residence times (12 d or 20 d) were adopted as well as two different feed frequencies (1 and 4 d-1, for each culture residence time), the volumetric organic load rate being the same under all conditions. The transient response of the microorganism to the periodic acetate feed was investigated through batch tests with biomass withdrawn from the SBR, as function of the different SBR operating conditions. In all tested conditions, a quick transient response to the acetate spike was observed with fast increase of acetate uptake rate (ranging from 71 to 247 mgCOD gCOD-1 h-1). This transient response was mainly due to acetate storage in form of poly-hydroxybutyrate (ranging from 45% to 64% of the observed yield) whereas the growth response (i.e. increase of production rate of active biomass) generally played a minor role (ranging from 21% to 38% of the observed yield). Apart from this general trend, culture residence time as well as feed frequency had a strong impact on transient behaviour of cultured cells. The overall transient response (i.e. maximum specific substrate removal rate) increased as culture residence time decreased or as feed frequency increased. Moreover, the ratio of storage response and growth response increased as the overall transient response decreased, i.e. the storage response was preferentially maintained when cells presented a lower transient response. The ability of the cells to increase their growth rate with respect to SBR average value was the lowest under the most unfavourable conditions (residence time 20 d, feed frequency 1 d-1) and increased with the increase in maximum substrate uptake rate.

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