Effect of prednisone on protease activities and structural protein levels in rat muscles in vivo

J W Haycock, G Falkous, C A Maltin, Margaret Inkster Delday, D Mantle

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

To further elucidate the biochemical mechanism by which the corticosteroid prednisone induces differential changes in muscle mass (via altered protein synthesis/degradation rates) in normal or degenerating muscle tissues, we have determined the activity of a range of proteolytic enzyme types, together with levels of muscle structural proteins, in five innervated and denervated muscle types from control and drug treated rats, In both normal and wasting muscles, the activity of many protease types was substantially down-regulated following treatment with prednisone; however, accompanying net decreases in muscle mass were observed (although the structural protein composition of muscles was unaltered following drug treatment), We conclude that whilst overall rates of protein degradation in both normal and degenerating muscle may be reduced (via protease down-regulation) following prednisone treatment, the effect of the latter in reducing protein synthesis rates must be proportionately greater (even in actively degenerating tissue), Thus, the data do not support the hypothesis that the beneficial effect of prednisone in maintaining muscle mass in pathological tissues (e.g., Duchenne muscular dystrophy (DMD)) operates principally via down-regulation of protease action/protein catabolism.

Original languageEnglish
Pages (from-to)47-58
Number of pages12
JournalClinica Chimica Acta
Volume249
Issue number1-2
DOIs
Publication statusPublished - 30 May 1996

Keywords

  • muscle proteins
  • prednisone
  • muscular dystrophy
  • protein metabolism
  • proteases
  • denervation
  • DUCHENNE MUSCULAR-DYSTROPHY
  • CORTICOSTEROID-THERAPY
  • SKELETAL-MUSCLE
  • CLENBUTEROL
  • BREAKDOWN
  • MYOPATHY
  • INVIVO

Cite this

Effect of prednisone on protease activities and structural protein levels in rat muscles in vivo. / Haycock, J W ; Falkous, G ; Maltin, C A ; Delday, Margaret Inkster; Mantle, D .

In: Clinica Chimica Acta, Vol. 249, No. 1-2, 30.05.1996, p. 47-58.

Research output: Contribution to journalArticle

Haycock, J W ; Falkous, G ; Maltin, C A ; Delday, Margaret Inkster ; Mantle, D . / Effect of prednisone on protease activities and structural protein levels in rat muscles in vivo. In: Clinica Chimica Acta. 1996 ; Vol. 249, No. 1-2. pp. 47-58.
@article{896512504d964447808d806b0290fdfa,
title = "Effect of prednisone on protease activities and structural protein levels in rat muscles in vivo",
abstract = "To further elucidate the biochemical mechanism by which the corticosteroid prednisone induces differential changes in muscle mass (via altered protein synthesis/degradation rates) in normal or degenerating muscle tissues, we have determined the activity of a range of proteolytic enzyme types, together with levels of muscle structural proteins, in five innervated and denervated muscle types from control and drug treated rats, In both normal and wasting muscles, the activity of many protease types was substantially down-regulated following treatment with prednisone; however, accompanying net decreases in muscle mass were observed (although the structural protein composition of muscles was unaltered following drug treatment), We conclude that whilst overall rates of protein degradation in both normal and degenerating muscle may be reduced (via protease down-regulation) following prednisone treatment, the effect of the latter in reducing protein synthesis rates must be proportionately greater (even in actively degenerating tissue), Thus, the data do not support the hypothesis that the beneficial effect of prednisone in maintaining muscle mass in pathological tissues (e.g., Duchenne muscular dystrophy (DMD)) operates principally via down-regulation of protease action/protein catabolism.",
keywords = "muscle proteins, prednisone, muscular dystrophy, protein metabolism, proteases, denervation, DUCHENNE MUSCULAR-DYSTROPHY, CORTICOSTEROID-THERAPY, SKELETAL-MUSCLE, CLENBUTEROL, BREAKDOWN, MYOPATHY, INVIVO",
author = "Haycock, {J W} and G Falkous and Maltin, {C A} and Delday, {Margaret Inkster} and D Mantle",
year = "1996",
month = "5",
day = "30",
doi = "10.1016/0009-8981(95)06257-2",
language = "English",
volume = "249",
pages = "47--58",
journal = "Clinica Chimica Acta",
issn = "0009-8981",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Effect of prednisone on protease activities and structural protein levels in rat muscles in vivo

AU - Haycock, J W

AU - Falkous, G

AU - Maltin, C A

AU - Delday, Margaret Inkster

AU - Mantle, D

PY - 1996/5/30

Y1 - 1996/5/30

N2 - To further elucidate the biochemical mechanism by which the corticosteroid prednisone induces differential changes in muscle mass (via altered protein synthesis/degradation rates) in normal or degenerating muscle tissues, we have determined the activity of a range of proteolytic enzyme types, together with levels of muscle structural proteins, in five innervated and denervated muscle types from control and drug treated rats, In both normal and wasting muscles, the activity of many protease types was substantially down-regulated following treatment with prednisone; however, accompanying net decreases in muscle mass were observed (although the structural protein composition of muscles was unaltered following drug treatment), We conclude that whilst overall rates of protein degradation in both normal and degenerating muscle may be reduced (via protease down-regulation) following prednisone treatment, the effect of the latter in reducing protein synthesis rates must be proportionately greater (even in actively degenerating tissue), Thus, the data do not support the hypothesis that the beneficial effect of prednisone in maintaining muscle mass in pathological tissues (e.g., Duchenne muscular dystrophy (DMD)) operates principally via down-regulation of protease action/protein catabolism.

AB - To further elucidate the biochemical mechanism by which the corticosteroid prednisone induces differential changes in muscle mass (via altered protein synthesis/degradation rates) in normal or degenerating muscle tissues, we have determined the activity of a range of proteolytic enzyme types, together with levels of muscle structural proteins, in five innervated and denervated muscle types from control and drug treated rats, In both normal and wasting muscles, the activity of many protease types was substantially down-regulated following treatment with prednisone; however, accompanying net decreases in muscle mass were observed (although the structural protein composition of muscles was unaltered following drug treatment), We conclude that whilst overall rates of protein degradation in both normal and degenerating muscle may be reduced (via protease down-regulation) following prednisone treatment, the effect of the latter in reducing protein synthesis rates must be proportionately greater (even in actively degenerating tissue), Thus, the data do not support the hypothesis that the beneficial effect of prednisone in maintaining muscle mass in pathological tissues (e.g., Duchenne muscular dystrophy (DMD)) operates principally via down-regulation of protease action/protein catabolism.

KW - muscle proteins

KW - prednisone

KW - muscular dystrophy

KW - protein metabolism

KW - proteases

KW - denervation

KW - DUCHENNE MUSCULAR-DYSTROPHY

KW - CORTICOSTEROID-THERAPY

KW - SKELETAL-MUSCLE

KW - CLENBUTEROL

KW - BREAKDOWN

KW - MYOPATHY

KW - INVIVO

U2 - 10.1016/0009-8981(95)06257-2

DO - 10.1016/0009-8981(95)06257-2

M3 - Article

VL - 249

SP - 47

EP - 58

JO - Clinica Chimica Acta

JF - Clinica Chimica Acta

SN - 0009-8981

IS - 1-2

ER -