Effect of repeated exposure to AQUI-S® on the viability and growth of Neoparamoeba perurans

Rachel Chance, Zoe Allcock, Christopher Secombes, Bertrand Collet, Catherine Collins

Research output: Contribution to journalArticle

2 Citations (Scopus)
6 Downloads (Pure)

Abstract

There have been recent efforts amongst immunologists to develop approaches for following individual fish during challenges with viral and bacterial pathogens. This study contributes to assessing the feasibility of using such approaches to study amoebic gill disease (AGD). Neoparamoeba perurans, agent of AGD, has been responsible for widespread economic and fish loss in salmonid aquaculture. With the emergence of AGD in Europe, research into infection dynamics and host response has increased. This study investigated the effect of repeat exposure to anaesthesia, a necessary requirement when following disease progression in individual fish, on N. perurans. In vitro cultures of N. perurans were exposed every 4 days over a 28-day period to AQUI-S® (isoeugenol), a popular anaesthetic choice for AGD challenges, at a concentration and duration required to sedate post-smolt salmonids. Population growth was measured by sequential counts of amoeba over the period, while viability of non-attached amoeba in the culture was assessed with a vital stain. AQUI-S® was found to be a suitable choice for in vivo ectoparasitic challenges with N. perurans during which repetitive anaesthesia is required for analysis of disease progression.
Original languageEnglish
Pages (from-to)291-298
Number of pages8
JournalJournal of Fish Diseases
Volume41
Issue number2
Early online date25 Sep 2017
DOIs
Publication statusPublished - Feb 2018

Fingerprint

gills
viability
Fishes
isoeugenol
Amoeba
Growth
disease course
Disease Progression
anesthesia
Anesthesia
fish
Salmonidae
Aquaculture
Population Growth
smolts
in vitro culture
anesthetics
Anesthetics
aquaculture
anesthetic

Keywords

  • amoebic gill disease
  • anaesthesia
  • AQUI-S®
  • isoeugenol
  • Neoparamoeba perurans

Cite this

Effect of repeated exposure to AQUI-S® on the viability and growth of Neoparamoeba perurans. / Chance, Rachel; Allcock, Zoe; Secombes, Christopher; Collet, Bertrand; Collins, Catherine.

In: Journal of Fish Diseases, Vol. 41, No. 2, 02.2018, p. 291-298.

Research output: Contribution to journalArticle

Chance, Rachel ; Allcock, Zoe ; Secombes, Christopher ; Collet, Bertrand ; Collins, Catherine. / Effect of repeated exposure to AQUI-S® on the viability and growth of Neoparamoeba perurans. In: Journal of Fish Diseases. 2018 ; Vol. 41, No. 2. pp. 291-298.
@article{d41192a71fa34b4fb6380b7e26cad251,
title = "Effect of repeated exposure to AQUI-S{\circledR} on the viability and growth of Neoparamoeba perurans",
abstract = "There have been recent efforts amongst immunologists to develop approaches for following individual fish during challenges with viral and bacterial pathogens. This study contributes to assessing the feasibility of using such approaches to study amoebic gill disease (AGD). Neoparamoeba perurans, agent of AGD, has been responsible for widespread economic and fish loss in salmonid aquaculture. With the emergence of AGD in Europe, research into infection dynamics and host response has increased. This study investigated the effect of repeat exposure to anaesthesia, a necessary requirement when following disease progression in individual fish, on N. perurans. In vitro cultures of N. perurans were exposed every 4 days over a 28-day period to AQUI-S{\circledR} (isoeugenol), a popular anaesthetic choice for AGD challenges, at a concentration and duration required to sedate post-smolt salmonids. Population growth was measured by sequential counts of amoeba over the period, while viability of non-attached amoeba in the culture was assessed with a vital stain. AQUI-S{\circledR} was found to be a suitable choice for in vivo ectoparasitic challenges with N. perurans during which repetitive anaesthesia is required for analysis of disease progression.",
keywords = "amoebic gill disease, anaesthesia, AQUI-S{\circledR}, isoeugenol, Neoparamoeba perurans",
author = "Rachel Chance and Zoe Allcock and Christopher Secombes and Bertrand Collet and Catherine Collins",
year = "2018",
month = "2",
doi = "10.1111/jfd.12712",
language = "English",
volume = "41",
pages = "291--298",
journal = "Journal of Fish Diseases",
issn = "0140-7775",
publisher = "Wiley-Blackwell",
number = "2",

}

TY - JOUR

T1 - Effect of repeated exposure to AQUI-S® on the viability and growth of Neoparamoeba perurans

AU - Chance, Rachel

AU - Allcock, Zoe

AU - Secombes, Christopher

AU - Collet, Bertrand

AU - Collins, Catherine

PY - 2018/2

Y1 - 2018/2

N2 - There have been recent efforts amongst immunologists to develop approaches for following individual fish during challenges with viral and bacterial pathogens. This study contributes to assessing the feasibility of using such approaches to study amoebic gill disease (AGD). Neoparamoeba perurans, agent of AGD, has been responsible for widespread economic and fish loss in salmonid aquaculture. With the emergence of AGD in Europe, research into infection dynamics and host response has increased. This study investigated the effect of repeat exposure to anaesthesia, a necessary requirement when following disease progression in individual fish, on N. perurans. In vitro cultures of N. perurans were exposed every 4 days over a 28-day period to AQUI-S® (isoeugenol), a popular anaesthetic choice for AGD challenges, at a concentration and duration required to sedate post-smolt salmonids. Population growth was measured by sequential counts of amoeba over the period, while viability of non-attached amoeba in the culture was assessed with a vital stain. AQUI-S® was found to be a suitable choice for in vivo ectoparasitic challenges with N. perurans during which repetitive anaesthesia is required for analysis of disease progression.

AB - There have been recent efforts amongst immunologists to develop approaches for following individual fish during challenges with viral and bacterial pathogens. This study contributes to assessing the feasibility of using such approaches to study amoebic gill disease (AGD). Neoparamoeba perurans, agent of AGD, has been responsible for widespread economic and fish loss in salmonid aquaculture. With the emergence of AGD in Europe, research into infection dynamics and host response has increased. This study investigated the effect of repeat exposure to anaesthesia, a necessary requirement when following disease progression in individual fish, on N. perurans. In vitro cultures of N. perurans were exposed every 4 days over a 28-day period to AQUI-S® (isoeugenol), a popular anaesthetic choice for AGD challenges, at a concentration and duration required to sedate post-smolt salmonids. Population growth was measured by sequential counts of amoeba over the period, while viability of non-attached amoeba in the culture was assessed with a vital stain. AQUI-S® was found to be a suitable choice for in vivo ectoparasitic challenges with N. perurans during which repetitive anaesthesia is required for analysis of disease progression.

KW - amoebic gill disease

KW - anaesthesia

KW - AQUI-S®

KW - isoeugenol

KW - Neoparamoeba perurans

U2 - 10.1111/jfd.12712

DO - 10.1111/jfd.12712

M3 - Article

VL - 41

SP - 291

EP - 298

JO - Journal of Fish Diseases

JF - Journal of Fish Diseases

SN - 0140-7775

IS - 2

ER -