Effect of short-term macrophage depletion in the development of posterior capsule opacification in rodents

N. Lois, R. Dawson, J. Townend, A. D. McKinnon, G. C. Smith, R. van't Hof, N. Van Rooijen, J. V. Forrester

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Abstract

Aim: To evaluate the role of macrophages in the development of posterior capsule opacification (PCO).

Methods: For this purpose, an extracapsular lens extraction was performed in 18 consecutive Sprague-Dawley rats. Animals were treated with liposomal clodronate (Cl2MDP-lip-treated group, n = 10) or phosphate-buffered saline (PBS) (control group, n = 8) 1 day preoperatively and on the first day postoperatively, and sacrificed 3 days postoperatively. Masked clinical, light microscopy and immunohistochemistry studies were conducted. The Fisher exact test and randomisation test were used to assess statistically differences between groups.

Results: A statistically significant reduction in the number of macrophages (ED1+, ED7+, ED8+) was found in the Cl2MDP-lip-treated group compared with the PBS-lip-treated group (p = 0.048, p = 0.004, p = 0.027, respectively). There were no statistically significant differences with regards to the presence/absence of central opacification (p = 0.29) and capsular wrinkling (p = 0.21) as detected clinically between groups. Similarly, a qualitative evaluation of the degree of PCO with regards to lens epithelial cell (LEC) proliferation, capsular wrinkling and Soemmerring ring formation showed no statistically significance between groups (p = 0.27, p = 0.061, p = 1.0, respectively). However, a statistically significant reduction in the number of lens epithelial cells (LEC) counted in the centre of the posterior capsule was found in the Cl2MDP-lip-treated group (p = 0.009).

Conclusion: Depletion of macrophages was accompanied by a reduction in LEC in the centre of the posterior capsule in rodents.

Original languageEnglish
Pages (from-to)1528-1533
Number of pages6
JournalBritish Journal of Ophthalmology
Volume92
Issue number11
Early online date23 Sep 2008
DOIs
Publication statusPublished - Nov 2008

Keywords

  • lens epithelial-cells
  • transforming growth-factor
  • smooth muscle actin
  • in-vivo
  • dichloromethylene-diphosphonate
  • proteasome inhibition
  • extracellular-matrix
  • down-regulation
  • expression
  • proliferation

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