Effect of short-term macrophage depletion in the development of posterior capsule opacification in rodents

N. Lois, R. Dawson, J. Townend, A. D. McKinnon, G. C. Smith, R. van't Hof, N. Van Rooijen, J. V. Forrester

Research output: Contribution to journalArticle

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Abstract

Aim: To evaluate the role of macrophages in the development of posterior capsule opacification (PCO).

Methods: For this purpose, an extracapsular lens extraction was performed in 18 consecutive Sprague-Dawley rats. Animals were treated with liposomal clodronate (Cl2MDP-lip-treated group, n = 10) or phosphate-buffered saline (PBS) (control group, n = 8) 1 day preoperatively and on the first day postoperatively, and sacrificed 3 days postoperatively. Masked clinical, light microscopy and immunohistochemistry studies were conducted. The Fisher exact test and randomisation test were used to assess statistically differences between groups.

Results: A statistically significant reduction in the number of macrophages (ED1+, ED7+, ED8+) was found in the Cl2MDP-lip-treated group compared with the PBS-lip-treated group (p = 0.048, p = 0.004, p = 0.027, respectively). There were no statistically significant differences with regards to the presence/absence of central opacification (p = 0.29) and capsular wrinkling (p = 0.21) as detected clinically between groups. Similarly, a qualitative evaluation of the degree of PCO with regards to lens epithelial cell (LEC) proliferation, capsular wrinkling and Soemmerring ring formation showed no statistically significance between groups (p = 0.27, p = 0.061, p = 1.0, respectively). However, a statistically significant reduction in the number of lens epithelial cells (LEC) counted in the centre of the posterior capsule was found in the Cl2MDP-lip-treated group (p = 0.009).

Conclusion: Depletion of macrophages was accompanied by a reduction in LEC in the centre of the posterior capsule in rodents.

Original languageEnglish
Pages (from-to)1528-1533
Number of pages6
JournalBritish Journal of Ophthalmology
Volume92
Issue number11
Early online date23 Sep 2008
DOIs
Publication statusPublished - Nov 2008

Keywords

  • lens epithelial-cells
  • transforming growth-factor
  • smooth muscle actin
  • in-vivo
  • dichloromethylene-diphosphonate
  • proteasome inhibition
  • extracellular-matrix
  • down-regulation
  • expression
  • proliferation

Cite this

Lois, N., Dawson, R., Townend, J., McKinnon, A. D., Smith, G. C., van't Hof, R., ... Forrester, J. V. (2008). Effect of short-term macrophage depletion in the development of posterior capsule opacification in rodents. British Journal of Ophthalmology, 92(11), 1528-1533. https://doi.org/10.1136/bjo.2007.130518

Effect of short-term macrophage depletion in the development of posterior capsule opacification in rodents. / Lois, N.; Dawson, R.; Townend, J.; McKinnon, A. D.; Smith, G. C.; van't Hof, R.; Van Rooijen, N.; Forrester, J. V.

In: British Journal of Ophthalmology, Vol. 92, No. 11, 11.2008, p. 1528-1533.

Research output: Contribution to journalArticle

Lois, N, Dawson, R, Townend, J, McKinnon, AD, Smith, GC, van't Hof, R, Van Rooijen, N & Forrester, JV 2008, 'Effect of short-term macrophage depletion in the development of posterior capsule opacification in rodents', British Journal of Ophthalmology, vol. 92, no. 11, pp. 1528-1533. https://doi.org/10.1136/bjo.2007.130518
Lois, N. ; Dawson, R. ; Townend, J. ; McKinnon, A. D. ; Smith, G. C. ; van't Hof, R. ; Van Rooijen, N. ; Forrester, J. V. / Effect of short-term macrophage depletion in the development of posterior capsule opacification in rodents. In: British Journal of Ophthalmology. 2008 ; Vol. 92, No. 11. pp. 1528-1533.
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T1 - Effect of short-term macrophage depletion in the development of posterior capsule opacification in rodents

AU - Lois, N.

AU - Dawson, R.

AU - Townend, J.

AU - McKinnon, A. D.

AU - Smith, G. C.

AU - van't Hof, R.

AU - Van Rooijen, N.

AU - Forrester, J. V.

PY - 2008/11

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N2 - Aim: To evaluate the role of macrophages in the development of posterior capsule opacification (PCO).Methods: For this purpose, an extracapsular lens extraction was performed in 18 consecutive Sprague-Dawley rats. Animals were treated with liposomal clodronate (Cl2MDP-lip-treated group, n = 10) or phosphate-buffered saline (PBS) (control group, n = 8) 1 day preoperatively and on the first day postoperatively, and sacrificed 3 days postoperatively. Masked clinical, light microscopy and immunohistochemistry studies were conducted. The Fisher exact test and randomisation test were used to assess statistically differences between groups.Results: A statistically significant reduction in the number of macrophages (ED1+, ED7+, ED8+) was found in the Cl2MDP-lip-treated group compared with the PBS-lip-treated group (p = 0.048, p = 0.004, p = 0.027, respectively). There were no statistically significant differences with regards to the presence/absence of central opacification (p = 0.29) and capsular wrinkling (p = 0.21) as detected clinically between groups. Similarly, a qualitative evaluation of the degree of PCO with regards to lens epithelial cell (LEC) proliferation, capsular wrinkling and Soemmerring ring formation showed no statistically significance between groups (p = 0.27, p = 0.061, p = 1.0, respectively). However, a statistically significant reduction in the number of lens epithelial cells (LEC) counted in the centre of the posterior capsule was found in the Cl2MDP-lip-treated group (p = 0.009).Conclusion: Depletion of macrophages was accompanied by a reduction in LEC in the centre of the posterior capsule in rodents.

AB - Aim: To evaluate the role of macrophages in the development of posterior capsule opacification (PCO).Methods: For this purpose, an extracapsular lens extraction was performed in 18 consecutive Sprague-Dawley rats. Animals were treated with liposomal clodronate (Cl2MDP-lip-treated group, n = 10) or phosphate-buffered saline (PBS) (control group, n = 8) 1 day preoperatively and on the first day postoperatively, and sacrificed 3 days postoperatively. Masked clinical, light microscopy and immunohistochemistry studies were conducted. The Fisher exact test and randomisation test were used to assess statistically differences between groups.Results: A statistically significant reduction in the number of macrophages (ED1+, ED7+, ED8+) was found in the Cl2MDP-lip-treated group compared with the PBS-lip-treated group (p = 0.048, p = 0.004, p = 0.027, respectively). There were no statistically significant differences with regards to the presence/absence of central opacification (p = 0.29) and capsular wrinkling (p = 0.21) as detected clinically between groups. Similarly, a qualitative evaluation of the degree of PCO with regards to lens epithelial cell (LEC) proliferation, capsular wrinkling and Soemmerring ring formation showed no statistically significance between groups (p = 0.27, p = 0.061, p = 1.0, respectively). However, a statistically significant reduction in the number of lens epithelial cells (LEC) counted in the centre of the posterior capsule was found in the Cl2MDP-lip-treated group (p = 0.009).Conclusion: Depletion of macrophages was accompanied by a reduction in LEC in the centre of the posterior capsule in rodents.

KW - lens epithelial-cells

KW - transforming growth-factor

KW - smooth muscle actin

KW - in-vivo

KW - dichloromethylene-diphosphonate

KW - proteasome inhibition

KW - extracellular-matrix

KW - down-regulation

KW - expression

KW - proliferation

U2 - 10.1136/bjo.2007.130518

DO - 10.1136/bjo.2007.130518

M3 - Article

VL - 92

SP - 1528

EP - 1533

JO - British Journal of Ophthalmology

JF - British Journal of Ophthalmology

SN - 0007-1161

IS - 11

ER -