EFFECT OF SOIL BULK-DENSITY AND TEMPERATURE ON WHEAT RHIZOSPHERE COLONIZATION BY LUX-MARKED PSEUDOMONAS-FLUORESCENS

Elizabeth-Ann Simpson Rattray, J A TYRRELL, James Ivor Prosser, Lesley Anne Glover, Kenneth Stuart Killham

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The effects of bulk density and temperature on the rates of colonisation of the wheat rhizosphere and establishment in bulk soil were investigated by inoculating lux-marked Pseudomonas fluorescens onto seeds. The coated seeds were placed in soil microcosms, maintained at a constant matric potential. The wheat seedlings were harvested every 7 days over a 28 days period. Survival and colonisation were determined over a 28 days period by performing dilution plate counts on seed, above-ground material, ectorhizosphere, endorhizosphere and bulk soil samples. Selection of the marked inoculum was made on the basis or resistance to kanamycin and rifampicin, with the ability to emit light. Soil bulk density and temperature had a significant effect on colonisation of the rhizosphere, with greatest rates of colonisation occurring at the lower bulk density (0.82 g cm(-3)) and the highest temperature (22 degrees C). Colony forming units (CFU) were approximately 100-fold higher in the ectorhizosphere than in the endorhizosphere. Colonisation of the above-ground material was affected by temperature and was unaffected by changes in bulk density. Populations of lux-marked cells were 2-3 orders of magnitude greater at 8 degrees C than at 22 degrees C.

Original languageEnglish
Pages (from-to)73-82
Number of pages10
JournalEuropean Journal of Soil Biology
Volume29
Issue number2
Publication statusPublished - 1993

Keywords

  • RHIZOSPHERE COLONIZATION
  • BIOLUMINESCENCE
  • LUX-MARKER INOCULUM
  • BIOLOGICAL-CONTROL
  • ROOT COLONIZATION
  • ESCHERICHIA-COLI
  • TAKE-ALL
  • BACTERIA
  • SURVIVAL
  • ENVIRONMENT
  • MICROFLORA
  • PATHOGENS

Cite this

EFFECT OF SOIL BULK-DENSITY AND TEMPERATURE ON WHEAT RHIZOSPHERE COLONIZATION BY LUX-MARKED PSEUDOMONAS-FLUORESCENS. / Rattray, Elizabeth-Ann Simpson; TYRRELL, J A ; Prosser, James Ivor; Glover, Lesley Anne; Killham, Kenneth Stuart.

In: European Journal of Soil Biology, Vol. 29, No. 2, 1993, p. 73-82.

Research output: Contribution to journalArticle

Rattray, Elizabeth-Ann Simpson ; TYRRELL, J A ; Prosser, James Ivor ; Glover, Lesley Anne ; Killham, Kenneth Stuart. / EFFECT OF SOIL BULK-DENSITY AND TEMPERATURE ON WHEAT RHIZOSPHERE COLONIZATION BY LUX-MARKED PSEUDOMONAS-FLUORESCENS. In: European Journal of Soil Biology. 1993 ; Vol. 29, No. 2. pp. 73-82.
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abstract = "The effects of bulk density and temperature on the rates of colonisation of the wheat rhizosphere and establishment in bulk soil were investigated by inoculating lux-marked Pseudomonas fluorescens onto seeds. The coated seeds were placed in soil microcosms, maintained at a constant matric potential. The wheat seedlings were harvested every 7 days over a 28 days period. Survival and colonisation were determined over a 28 days period by performing dilution plate counts on seed, above-ground material, ectorhizosphere, endorhizosphere and bulk soil samples. Selection of the marked inoculum was made on the basis or resistance to kanamycin and rifampicin, with the ability to emit light. Soil bulk density and temperature had a significant effect on colonisation of the rhizosphere, with greatest rates of colonisation occurring at the lower bulk density (0.82 g cm(-3)) and the highest temperature (22 degrees C). Colony forming units (CFU) were approximately 100-fold higher in the ectorhizosphere than in the endorhizosphere. Colonisation of the above-ground material was affected by temperature and was unaffected by changes in bulk density. Populations of lux-marked cells were 2-3 orders of magnitude greater at 8 degrees C than at 22 degrees C.",
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AB - The effects of bulk density and temperature on the rates of colonisation of the wheat rhizosphere and establishment in bulk soil were investigated by inoculating lux-marked Pseudomonas fluorescens onto seeds. The coated seeds were placed in soil microcosms, maintained at a constant matric potential. The wheat seedlings were harvested every 7 days over a 28 days period. Survival and colonisation were determined over a 28 days period by performing dilution plate counts on seed, above-ground material, ectorhizosphere, endorhizosphere and bulk soil samples. Selection of the marked inoculum was made on the basis or resistance to kanamycin and rifampicin, with the ability to emit light. Soil bulk density and temperature had a significant effect on colonisation of the rhizosphere, with greatest rates of colonisation occurring at the lower bulk density (0.82 g cm(-3)) and the highest temperature (22 degrees C). Colony forming units (CFU) were approximately 100-fold higher in the ectorhizosphere than in the endorhizosphere. Colonisation of the above-ground material was affected by temperature and was unaffected by changes in bulk density. Populations of lux-marked cells were 2-3 orders of magnitude greater at 8 degrees C than at 22 degrees C.

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KW - ESCHERICHIA-COLI

KW - TAKE-ALL

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