Effect of Sunflower and Marine Oils on Ruminal Microbiota, In vitro Fermentation and Digesta Fatty Acid Profile

Julio E. Vargas, Sonia Andres, Timothy J. Snelling, Lorena Lopez-Ferreras, David R. Yanez-Ruiz, Carlos Garcia-Estrada, Secundino Lopez

Research output: Contribution to journalArticle

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Abstract

This study using the rumen simulation technique (RUSITEC) investigated the changes in the ruminal microbiota and anaerobic fermentation in response to the addition of different lipid supplements to a ruminant diet. A basal diet with no oil added was the control, and the treatment diets were supplemented with sunflower oil (2%) only, or sunflower oil (2%) in combination with fish oil (1%) or algae oil (1%). Four fermentation units were used per treatment. RUSITEC fermenters were inoculated with rumen digesta. Substrate degradation, fermentation end-products (volatile fatty acids, lactate, gas, methane, and ammonia), and microbial protein synthesis were determined. Fatty acid profiles and microbial community composition were evaluated in digesta samples. Numbers of representative bacterial species and microbial groups were determined using qPCR. Microbial composition and diversity were based on T-RFLP spectra. The addition of oils had no effect on substrate degradation or microbial protein synthesis. Differences among diets in neutral detergent fiber degradation were not significant (P = 0.132), but the contrast comparing oil–supplemented diets with the control was significant (P= 0.039). Methane production was reduced (P < 0.05) with all oil supplements. Propionate production was increased when diets containing oil were fermented. Compared with the control, the addition of algae oil decreased the percentage C18:3 c9c12c15 in rumen digesta, and that of C18:2 c9t11 was increased when the control diet was supplemented with any oil. Marine oils decreased the hydrogenation of C18 unsaturated fatty acids. Microbial diversity was not affected by oil supplementation. Cluster analysis showed that diets with additional fish or algae oils formed a group separated from the sunflower oil diet. Supplementation with marine oils decreased the numbers of Butyrivibrio producers of stearic acid, and affected the numbers of protozoa, methanogens, Selenomonas ruminantium and Streptococcus bovis, but not total bacteria. In conclusion, there is a potential to manipulate the rumen fermentation and microbiota with the addition of sunflower, fish or algae oils to ruminant diets at appropriate concentrations. Specifically, supplementation of ruminant mixed rations with marine oils will reduce methane production, the acetate to propionate ratio and the fatty acid hydrogenation in the rumen.
Original languageEnglish
Article number1124
Pages (from-to)1-15
Number of pages15
JournalFrontiers in Microbiology
Volume8
DOIs
Publication statusPublished - 20 Jun 2017

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Microbiota
Fermentation
Oils
Fatty Acids
Diet
Rumen
Methane
Ruminants
Hydrogenation
Propionates
In Vitro Techniques
sunflower seed oil
Fishes
Butyrivibrio
Selenomonas
Streptococcus bovis
Fish Oils
Volatile Fatty Acids
Helianthus
Unsaturated Fatty Acids

Keywords

  • microbial community composition
  • dietary fats
  • rumen microbiota
  • Rusitec fermenters
  • TRFLP
  • qPCR

Cite this

Vargas, J. E., Andres, S., Snelling, T. J., Lopez-Ferreras, L., Yanez-Ruiz, D. R., Garcia-Estrada, C., & Lopez, S. (2017). Effect of Sunflower and Marine Oils on Ruminal Microbiota, In vitro Fermentation and Digesta Fatty Acid Profile. Frontiers in Microbiology, 8, 1-15. [1124]. https://doi.org/10.3389/fmicb.2017.01124

Effect of Sunflower and Marine Oils on Ruminal Microbiota, In vitro Fermentation and Digesta Fatty Acid Profile. / Vargas, Julio E.; Andres, Sonia; Snelling, Timothy J.; Lopez-Ferreras, Lorena; Yanez-Ruiz, David R.; Garcia-Estrada, Carlos; Lopez, Secundino.

In: Frontiers in Microbiology, Vol. 8, 1124, 20.06.2017, p. 1-15.

Research output: Contribution to journalArticle

Vargas, JE, Andres, S, Snelling, TJ, Lopez-Ferreras, L, Yanez-Ruiz, DR, Garcia-Estrada, C & Lopez, S 2017, 'Effect of Sunflower and Marine Oils on Ruminal Microbiota, In vitro Fermentation and Digesta Fatty Acid Profile', Frontiers in Microbiology, vol. 8, 1124, pp. 1-15. https://doi.org/10.3389/fmicb.2017.01124
Vargas JE, Andres S, Snelling TJ, Lopez-Ferreras L, Yanez-Ruiz DR, Garcia-Estrada C et al. Effect of Sunflower and Marine Oils on Ruminal Microbiota, In vitro Fermentation and Digesta Fatty Acid Profile. Frontiers in Microbiology. 2017 Jun 20;8:1-15. 1124. https://doi.org/10.3389/fmicb.2017.01124
Vargas, Julio E. ; Andres, Sonia ; Snelling, Timothy J. ; Lopez-Ferreras, Lorena ; Yanez-Ruiz, David R. ; Garcia-Estrada, Carlos ; Lopez, Secundino. / Effect of Sunflower and Marine Oils on Ruminal Microbiota, In vitro Fermentation and Digesta Fatty Acid Profile. In: Frontiers in Microbiology. 2017 ; Vol. 8. pp. 1-15.
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T1 - Effect of Sunflower and Marine Oils on Ruminal Microbiota, In vitro Fermentation and Digesta Fatty Acid Profile

AU - Vargas, Julio E.

AU - Andres, Sonia

AU - Snelling, Timothy J.

AU - Lopez-Ferreras, Lorena

AU - Yanez-Ruiz, David R.

AU - Garcia-Estrada, Carlos

AU - Lopez, Secundino

N1 - Funding This work has been funded by Consejería de Educación, Junta de Castilla y León (research project LE007A07). Acknowledgments We acknowledge support of the publication fee by the CSIC Open Access Publication Support Initiative through its Unit of Information Resources for Research (URICI). Support received from CICYT project AGL2005-04760-C02-02 is gratefully acknowledged.

PY - 2017/6/20

Y1 - 2017/6/20

N2 - This study using the rumen simulation technique (RUSITEC) investigated the changes in the ruminal microbiota and anaerobic fermentation in response to the addition of different lipid supplements to a ruminant diet. A basal diet with no oil added was the control, and the treatment diets were supplemented with sunflower oil (2%) only, or sunflower oil (2%) in combination with fish oil (1%) or algae oil (1%). Four fermentation units were used per treatment. RUSITEC fermenters were inoculated with rumen digesta. Substrate degradation, fermentation end-products (volatile fatty acids, lactate, gas, methane, and ammonia), and microbial protein synthesis were determined. Fatty acid profiles and microbial community composition were evaluated in digesta samples. Numbers of representative bacterial species and microbial groups were determined using qPCR. Microbial composition and diversity were based on T-RFLP spectra. The addition of oils had no effect on substrate degradation or microbial protein synthesis. Differences among diets in neutral detergent fiber degradation were not significant (P = 0.132), but the contrast comparing oil–supplemented diets with the control was significant (P= 0.039). Methane production was reduced (P < 0.05) with all oil supplements. Propionate production was increased when diets containing oil were fermented. Compared with the control, the addition of algae oil decreased the percentage C18:3 c9c12c15 in rumen digesta, and that of C18:2 c9t11 was increased when the control diet was supplemented with any oil. Marine oils decreased the hydrogenation of C18 unsaturated fatty acids. Microbial diversity was not affected by oil supplementation. Cluster analysis showed that diets with additional fish or algae oils formed a group separated from the sunflower oil diet. Supplementation with marine oils decreased the numbers of Butyrivibrio producers of stearic acid, and affected the numbers of protozoa, methanogens, Selenomonas ruminantium and Streptococcus bovis, but not total bacteria. In conclusion, there is a potential to manipulate the rumen fermentation and microbiota with the addition of sunflower, fish or algae oils to ruminant diets at appropriate concentrations. Specifically, supplementation of ruminant mixed rations with marine oils will reduce methane production, the acetate to propionate ratio and the fatty acid hydrogenation in the rumen.

AB - This study using the rumen simulation technique (RUSITEC) investigated the changes in the ruminal microbiota and anaerobic fermentation in response to the addition of different lipid supplements to a ruminant diet. A basal diet with no oil added was the control, and the treatment diets were supplemented with sunflower oil (2%) only, or sunflower oil (2%) in combination with fish oil (1%) or algae oil (1%). Four fermentation units were used per treatment. RUSITEC fermenters were inoculated with rumen digesta. Substrate degradation, fermentation end-products (volatile fatty acids, lactate, gas, methane, and ammonia), and microbial protein synthesis were determined. Fatty acid profiles and microbial community composition were evaluated in digesta samples. Numbers of representative bacterial species and microbial groups were determined using qPCR. Microbial composition and diversity were based on T-RFLP spectra. The addition of oils had no effect on substrate degradation or microbial protein synthesis. Differences among diets in neutral detergent fiber degradation were not significant (P = 0.132), but the contrast comparing oil–supplemented diets with the control was significant (P= 0.039). Methane production was reduced (P < 0.05) with all oil supplements. Propionate production was increased when diets containing oil were fermented. Compared with the control, the addition of algae oil decreased the percentage C18:3 c9c12c15 in rumen digesta, and that of C18:2 c9t11 was increased when the control diet was supplemented with any oil. Marine oils decreased the hydrogenation of C18 unsaturated fatty acids. Microbial diversity was not affected by oil supplementation. Cluster analysis showed that diets with additional fish or algae oils formed a group separated from the sunflower oil diet. Supplementation with marine oils decreased the numbers of Butyrivibrio producers of stearic acid, and affected the numbers of protozoa, methanogens, Selenomonas ruminantium and Streptococcus bovis, but not total bacteria. In conclusion, there is a potential to manipulate the rumen fermentation and microbiota with the addition of sunflower, fish or algae oils to ruminant diets at appropriate concentrations. Specifically, supplementation of ruminant mixed rations with marine oils will reduce methane production, the acetate to propionate ratio and the fatty acid hydrogenation in the rumen.

KW - microbial community composition

KW - dietary fats

KW - rumen microbiota

KW - Rusitec fermenters

KW - TRFLP

KW - qPCR

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DO - 10.3389/fmicb.2017.01124

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VL - 8

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EP - 15

JO - Frontiers in Microbiology

JF - Frontiers in Microbiology

SN - 1664-302X

M1 - 1124

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