Effect of supplementation with an 80:20 cis9, trans11 conjugated linoleic acid blend on the human platelet proteome

Eva-Maria Bachmair, Michiel L. Bots, Louise I. Mennen, Thomas Kelder, Chris T. Evelo, Graham W. Horgan, Isobel Ford, Baukje de Roos

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

SCOPE: The dietary fatty acid cis9,trans11 conjugated linoleic acid (cis9,trans11 CLA) has been shown to modify the function of endothelial cells, monocytes, and platelets, all of which are involved in the development of atherosclerosis. Potential mechanisms for the platelet effects have not been assessed previously. In this study, we assessed how supplementation of the diet with an 80:20 cis9,trans11 CLA blend affects the platelet proteome.

METHODS AND RESULTS: In a double-blind, randomized, placebo-controlled, parallel-group trial, 40 overweight but apparently healthy adults received either 4 g per day of cis9,trans11 CLA-enriched oil or placebo oil, consisting of palm oil and soybean oil, for 3 months. Total platelet proteins were extracted from washed platelets, separated using two-dimensional gel electrophoresis and differentially regulated protein spots were identified by LC-ESI-MS/MS. Supplementation with the CLA blend, compared with placebo, resulted in significant alterations in levels of 46 spots (p < 0.05), of which 40 were identified. Network analysis revealed that the majority of these proteins participate in regulation of the cytoskeleton and platelet structure, as well as receptor action, signaling, and focal adhesion.

CONCLUSION: The platelet proteomics approach revealed novel insights into regulation of cellular biomarkers of atherogenic and thrombotic pathways by an 80:20 cis9,trans11 CLA blend.
Original languageEnglish
Pages (from-to)1148-1159
Number of pages12
JournalMolecular Nutrition & Food Research
Volume56
Issue number7
Early online date30 May 2012
DOIs
Publication statusPublished - Jul 2012

Fingerprint

Conjugated Linoleic Acids
Proteome
conjugated linoleic acid
proteome
blended foods
Blood Platelets
placebos
Placebos
proteins
two-dimensional gel electrophoresis
Elaeis guineensis
palm oils
cytoskeleton
atherosclerosis
soybean oil
dietary fat
monocytes
proteomics
Oils
endothelial cells

Keywords

  • platelets
  • nutrition
  • intervention
  • proteomics
  • fatty acids
  • conjugated linoleic acid
  • cytoscape
  • dietary supplementation trial
  • platelet proteomics

Cite this

Effect of supplementation with an 80:20 cis9, trans11 conjugated linoleic acid blend on the human platelet proteome. / Bachmair, Eva-Maria; Bots, Michiel L.; Mennen, Louise I.; Kelder, Thomas; Evelo, Chris T.; Horgan, Graham W.; Ford, Isobel; de Roos, Baukje.

In: Molecular Nutrition & Food Research, Vol. 56, No. 7, 07.2012, p. 1148-1159.

Research output: Contribution to journalArticle

Bachmair, Eva-Maria ; Bots, Michiel L. ; Mennen, Louise I. ; Kelder, Thomas ; Evelo, Chris T. ; Horgan, Graham W. ; Ford, Isobel ; de Roos, Baukje. / Effect of supplementation with an 80:20 cis9, trans11 conjugated linoleic acid blend on the human platelet proteome. In: Molecular Nutrition & Food Research. 2012 ; Vol. 56, No. 7. pp. 1148-1159.
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AB - SCOPE: The dietary fatty acid cis9,trans11 conjugated linoleic acid (cis9,trans11 CLA) has been shown to modify the function of endothelial cells, monocytes, and platelets, all of which are involved in the development of atherosclerosis. Potential mechanisms for the platelet effects have not been assessed previously. In this study, we assessed how supplementation of the diet with an 80:20 cis9,trans11 CLA blend affects the platelet proteome.METHODS AND RESULTS: In a double-blind, randomized, placebo-controlled, parallel-group trial, 40 overweight but apparently healthy adults received either 4 g per day of cis9,trans11 CLA-enriched oil or placebo oil, consisting of palm oil and soybean oil, for 3 months. Total platelet proteins were extracted from washed platelets, separated using two-dimensional gel electrophoresis and differentially regulated protein spots were identified by LC-ESI-MS/MS. Supplementation with the CLA blend, compared with placebo, resulted in significant alterations in levels of 46 spots (p < 0.05), of which 40 were identified. Network analysis revealed that the majority of these proteins participate in regulation of the cytoskeleton and platelet structure, as well as receptor action, signaling, and focal adhesion.CONCLUSION: The platelet proteomics approach revealed novel insights into regulation of cellular biomarkers of atherogenic and thrombotic pathways by an 80:20 cis9,trans11 CLA blend.

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