Effect of the methodology on circulating peptides determination and consequences on net flux measurements across the gastrointestinal tract of sheep

L Bernard, C F R Backwell, D Rémond, David Wilson, Laura Leanne Bruce, Vivien Buchan, C MacRae

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Two methodologies for the measurement of peptide amino acids (PAA) in blood were compared to evaluate their effects on the measurement of the net flux of peptides across the gastrointestinal tract of sheep. These methods consisted of a chemical deproteinization of blood samples with sulfosalicylic acid (1.6 M, 0.1 ml for 1 ml of sample) or perchloric acid (1 M, 1 ml for 1 ml of sample) followed by ultrafiltration through a 3,000-Da cut-off filter (SSA + UF3 kD) or gel filtration through a Sephadex G-15 column (1,500-Da cut-off filter; PCA + G-15), respectively, prior to PAA analysis. Peptide concentrations as determined by amino acid concentrations before and after hydrolysis of samples were slightly greater with the SSA + UF3 kD (991 microM) than with the PCA + G-15 (605 microM) methodology. However, both methodologies gave similar net portal-drained viscera flux data in sheep fed on alfalfa pellets with histidine as the only significant uptake of peptide amino acid.
Original languageEnglish
Pages (from-to)13-21
Number of pages9
JournalArchiv für Tierernährung
Volume56
Issue number1
Publication statusPublished - 1 Feb 2002

Keywords

  • Amino Acids
  • Animals
  • Hydrolysis
  • Intestinal Absorption
  • Male
  • Molecular Weight
  • Peptides
  • Reproducibility of Results
  • Salicylic Acids
  • Sheep
  • Ultrafiltration

Fingerprint Dive into the research topics of 'Effect of the methodology on circulating peptides determination and consequences on net flux measurements across the gastrointestinal tract of sheep'. Together they form a unique fingerprint.

  • Cite this

    Bernard, L., Backwell, C. F. R., Rémond, D., Wilson, D., Bruce, L. L., Buchan, V., & MacRae, C. (2002). Effect of the methodology on circulating peptides determination and consequences on net flux measurements across the gastrointestinal tract of sheep. Archiv für Tierernährung, 56(1), 13-21.