Effect of the methodology on circulating peptides determination and consequences on net flux measurements across the gastrointestinal tract of sheep

L Bernard, C F R Backwell, D Rémond, David Wilson, Laura Leanne Bruce, Vivien Buchan, C MacRae

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Two methodologies for the measurement of peptide amino acids (PAA) in blood were compared to evaluate their effects on the measurement of the net flux of peptides across the gastrointestinal tract of sheep. These methods consisted of a chemical deproteinization of blood samples with sulfosalicylic acid (1.6 M, 0.1 ml for 1 ml of sample) or perchloric acid (1 M, 1 ml for 1 ml of sample) followed by ultrafiltration through a 3,000-Da cut-off filter (SSA + UF3 kD) or gel filtration through a Sephadex G-15 column (1,500-Da cut-off filter; PCA + G-15), respectively, prior to PAA analysis. Peptide concentrations as determined by amino acid concentrations before and after hydrolysis of samples were slightly greater with the SSA + UF3 kD (991 microM) than with the PCA + G-15 (605 microM) methodology. However, both methodologies gave similar net portal-drained viscera flux data in sheep fed on alfalfa pellets with histidine as the only significant uptake of peptide amino acid.
Original languageEnglish
Pages (from-to)13-21
Number of pages9
JournalArchiv für Tierernährung
Volume56
Issue number1
Publication statusPublished - 1 Feb 2002

Fingerprint

Gastrointestinal Tract
Sheep
Peptides
Amino Acids
Passive Cutaneous Anaphylaxis
Viscera
Medicago sativa
Ultrafiltration
Histidine
Gel Chromatography
Hydrolysis

Keywords

  • Amino Acids
  • Animals
  • Hydrolysis
  • Intestinal Absorption
  • Male
  • Molecular Weight
  • Peptides
  • Reproducibility of Results
  • Salicylic Acids
  • Sheep
  • Ultrafiltration

Cite this

Bernard, L., Backwell, C. F. R., Rémond, D., Wilson, D., Bruce, L. L., Buchan, V., & MacRae, C. (2002). Effect of the methodology on circulating peptides determination and consequences on net flux measurements across the gastrointestinal tract of sheep. Archiv für Tierernährung, 56(1), 13-21.

Effect of the methodology on circulating peptides determination and consequences on net flux measurements across the gastrointestinal tract of sheep. / Bernard, L; Backwell, C F R; Rémond, D; Wilson, David; Bruce, Laura Leanne; Buchan, Vivien; MacRae, C.

In: Archiv für Tierernährung, Vol. 56, No. 1, 01.02.2002, p. 13-21.

Research output: Contribution to journalArticle

Bernard, L, Backwell, CFR, Rémond, D, Wilson, D, Bruce, LL, Buchan, V & MacRae, C 2002, 'Effect of the methodology on circulating peptides determination and consequences on net flux measurements across the gastrointestinal tract of sheep', Archiv für Tierernährung, vol. 56, no. 1, pp. 13-21.
Bernard, L ; Backwell, C F R ; Rémond, D ; Wilson, David ; Bruce, Laura Leanne ; Buchan, Vivien ; MacRae, C. / Effect of the methodology on circulating peptides determination and consequences on net flux measurements across the gastrointestinal tract of sheep. In: Archiv für Tierernährung. 2002 ; Vol. 56, No. 1. pp. 13-21.
@article{d8b182f724314bdea8b980642c24d40d,
title = "Effect of the methodology on circulating peptides determination and consequences on net flux measurements across the gastrointestinal tract of sheep",
abstract = "Two methodologies for the measurement of peptide amino acids (PAA) in blood were compared to evaluate their effects on the measurement of the net flux of peptides across the gastrointestinal tract of sheep. These methods consisted of a chemical deproteinization of blood samples with sulfosalicylic acid (1.6 M, 0.1 ml for 1 ml of sample) or perchloric acid (1 M, 1 ml for 1 ml of sample) followed by ultrafiltration through a 3,000-Da cut-off filter (SSA + UF3 kD) or gel filtration through a Sephadex G-15 column (1,500-Da cut-off filter; PCA + G-15), respectively, prior to PAA analysis. Peptide concentrations as determined by amino acid concentrations before and after hydrolysis of samples were slightly greater with the SSA + UF3 kD (991 microM) than with the PCA + G-15 (605 microM) methodology. However, both methodologies gave similar net portal-drained viscera flux data in sheep fed on alfalfa pellets with histidine as the only significant uptake of peptide amino acid.",
keywords = "Amino Acids, Animals, Hydrolysis, Intestinal Absorption, Male, Molecular Weight, Peptides, Reproducibility of Results, Salicylic Acids, Sheep, Ultrafiltration",
author = "L Bernard and Backwell, {C F R} and D R{\'e}mond and David Wilson and Bruce, {Laura Leanne} and Vivien Buchan and C MacRae",
year = "2002",
month = "2",
day = "1",
language = "English",
volume = "56",
pages = "13--21",
journal = "Archiv f{\"u}r Tierern{\"a}hrung",
issn = "0003-942X",
publisher = "Taylor and Francis Ltd.",
number = "1",

}

TY - JOUR

T1 - Effect of the methodology on circulating peptides determination and consequences on net flux measurements across the gastrointestinal tract of sheep

AU - Bernard, L

AU - Backwell, C F R

AU - Rémond, D

AU - Wilson, David

AU - Bruce, Laura Leanne

AU - Buchan, Vivien

AU - MacRae, C

PY - 2002/2/1

Y1 - 2002/2/1

N2 - Two methodologies for the measurement of peptide amino acids (PAA) in blood were compared to evaluate their effects on the measurement of the net flux of peptides across the gastrointestinal tract of sheep. These methods consisted of a chemical deproteinization of blood samples with sulfosalicylic acid (1.6 M, 0.1 ml for 1 ml of sample) or perchloric acid (1 M, 1 ml for 1 ml of sample) followed by ultrafiltration through a 3,000-Da cut-off filter (SSA + UF3 kD) or gel filtration through a Sephadex G-15 column (1,500-Da cut-off filter; PCA + G-15), respectively, prior to PAA analysis. Peptide concentrations as determined by amino acid concentrations before and after hydrolysis of samples were slightly greater with the SSA + UF3 kD (991 microM) than with the PCA + G-15 (605 microM) methodology. However, both methodologies gave similar net portal-drained viscera flux data in sheep fed on alfalfa pellets with histidine as the only significant uptake of peptide amino acid.

AB - Two methodologies for the measurement of peptide amino acids (PAA) in blood were compared to evaluate their effects on the measurement of the net flux of peptides across the gastrointestinal tract of sheep. These methods consisted of a chemical deproteinization of blood samples with sulfosalicylic acid (1.6 M, 0.1 ml for 1 ml of sample) or perchloric acid (1 M, 1 ml for 1 ml of sample) followed by ultrafiltration through a 3,000-Da cut-off filter (SSA + UF3 kD) or gel filtration through a Sephadex G-15 column (1,500-Da cut-off filter; PCA + G-15), respectively, prior to PAA analysis. Peptide concentrations as determined by amino acid concentrations before and after hydrolysis of samples were slightly greater with the SSA + UF3 kD (991 microM) than with the PCA + G-15 (605 microM) methodology. However, both methodologies gave similar net portal-drained viscera flux data in sheep fed on alfalfa pellets with histidine as the only significant uptake of peptide amino acid.

KW - Amino Acids

KW - Animals

KW - Hydrolysis

KW - Intestinal Absorption

KW - Male

KW - Molecular Weight

KW - Peptides

KW - Reproducibility of Results

KW - Salicylic Acids

KW - Sheep

KW - Ultrafiltration

M3 - Article

VL - 56

SP - 13

EP - 21

JO - Archiv für Tierernährung

JF - Archiv für Tierernährung

SN - 0003-942X

IS - 1

ER -