Effect of the novel ionophore tetronasin (ICI 139603) on ruminal microorganisms

C. J. Newbold, R. J. Wallace, N. D. Watt, A. J. Richardson

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

The antimicrobial activity of the novel ionophore tetronasin (formerly ICI 139603) was compared with that of monensin for the growth of ruminal bacteria, protozoa, and an anaerobic fungus. The potency of tetronasin toward most bacteria and the fungus was an order of magnitude or more greater than that of monensin. Lactobacillus casei was 55 times more sensitive to tetronasin than to monensin, indicating a potential role for tetronasin in reversing lactic acidosis. Bacteria with a gram-positive ultrastructure were generally sensitive to the ionophores and unable to adapt to grow in their presence. The exception was the cellulolytic Ruminococcus flavefaciens, which adapted during succesive cultivation on media with increasing ionophore concentrations to grow at 100-fold higher concentrations of tetronasin than were initially lethal to the organism. Gram-negative bacteria were more resistant and generally able to adapt to grow in the presence of both ionophores. An in vivo trial with cattle and in vitro growth experiments indicated that the effect of tetronasin on ciliate protozoa was minor. In vitro experiments measuring hydrogen production by Neocallimastix frontalis suggested that this fungus would be unable to survive in ruminants receiving tetronasin.
Original languageEnglish
Pages (from-to)544-547
Number of pages4
JournalApplied and Environmental Microbiology
Volume54
Issue number2
DOIs
Publication statusPublished - Feb 1988

Fingerprint

rumen microorganisms
Ionophores
ionophores
monensin
microorganism
bacterium
fungus
Monensin
fungi
Protozoa
Neocallimastix frontalis
Ruminococcus flavefaciens
hydrogen production
rumen bacteria
Fungi
antimicrobial activity
Lactobacillus casei
ruminant
bacteria
acidosis

Cite this

Effect of the novel ionophore tetronasin (ICI 139603) on ruminal microorganisms. / Newbold, C. J.; Wallace, R. J.; Watt, N. D.; Richardson, A. J.

In: Applied and Environmental Microbiology, Vol. 54, No. 2, 02.1988, p. 544-547.

Research output: Contribution to journalArticle

Newbold, C. J. ; Wallace, R. J. ; Watt, N. D. ; Richardson, A. J. / Effect of the novel ionophore tetronasin (ICI 139603) on ruminal microorganisms. In: Applied and Environmental Microbiology. 1988 ; Vol. 54, No. 2. pp. 544-547.
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