Effect of the process conditions on the anaerobic fermentation of glucose for the production of chemicals

Davide Dionisi* (Corresponding Author), Victor Igwe, Graeme Paton

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

In the context of biorefinery for the production of chemicals, this study optimised the processes to enhance the anaerobic production of ethanol and short chain organic acids from glucose. The optimised variables included (with a total of 49 runs) residence time (2-100 h), temperature (25-35 OC), type of inoculum (soil or anaerobic digester), presence or absence of pre-acclimation of the inoculum, glucose concentration (5-50 g/L), continuous or batch mode and composition of the mineral media (mineral solution with or without trace elements, with deionised or tap water). In continuous experiments the residence time was the most important parameter that affected glucose conversion (over 80 % glucose conversion for residence time longer than 30 h) and product yields (ethanol was the main product in the range of residence times 20-50 h, with yields in the range 0.30-0.40 g/g glucose removed). Temperature, type of inoculum and pre-acclimation had little effect on glucose conversion and products yield. The addition of trace elements had an important beneficial effect on the removal of glucose when it was fed at the highest concentration (50 g/L) in both continuous and batch experiments. In batch runs acetate was generally the main fermentation product rather than ethanol and ethanol conversion into acetate was favoured by nitrogen sparging, probably due to the reduced hydrogen partial pressure.
Original languageEnglish
JournalBiomass Conversion and Biorefinery
Publication statusAccepted/In press - 18 Nov 2019

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fermentation
glucose
ethanol
residence time
acclimation
acetate
trace element
mineral
effect
chemical
partial pressure
organic acid
experiment
temperature
hydrogen
product
nitrogen
soil

Keywords

  • glucose
  • anaerobic fermentation
  • biorefinery
  • organic acids
  • ethanol

Cite this

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title = "Effect of the process conditions on the anaerobic fermentation of glucose for the production of chemicals",
abstract = "In the context of biorefinery for the production of chemicals, this study optimised the processes to enhance the anaerobic production of ethanol and short chain organic acids from glucose. The optimised variables included (with a total of 49 runs) residence time (2-100 h), temperature (25-35 OC), type of inoculum (soil or anaerobic digester), presence or absence of pre-acclimation of the inoculum, glucose concentration (5-50 g/L), continuous or batch mode and composition of the mineral media (mineral solution with or without trace elements, with deionised or tap water). In continuous experiments the residence time was the most important parameter that affected glucose conversion (over 80 {\%} glucose conversion for residence time longer than 30 h) and product yields (ethanol was the main product in the range of residence times 20-50 h, with yields in the range 0.30-0.40 g/g glucose removed). Temperature, type of inoculum and pre-acclimation had little effect on glucose conversion and products yield. The addition of trace elements had an important beneficial effect on the removal of glucose when it was fed at the highest concentration (50 g/L) in both continuous and batch experiments. In batch runs acetate was generally the main fermentation product rather than ethanol and ethanol conversion into acetate was favoured by nitrogen sparging, probably due to the reduced hydrogen partial pressure.",
keywords = "glucose, anaerobic fermentation, biorefinery, organic acids, ethanol",
author = "Davide Dionisi and Victor Igwe and Graeme Paton",
note = "Acknowledgments Victor Igwe acknowledges the financial help of Total E&P Nigeria Limited and Niger Delta Development Commission during his PhD studies.",
year = "2019",
month = "11",
day = "18",
language = "English",
journal = "Biomass Conversion and Biorefinery",
issn = "2190-6815",
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TY - JOUR

T1 - Effect of the process conditions on the anaerobic fermentation of glucose for the production of chemicals

AU - Dionisi, Davide

AU - Igwe, Victor

AU - Paton, Graeme

N1 - Acknowledgments Victor Igwe acknowledges the financial help of Total E&P Nigeria Limited and Niger Delta Development Commission during his PhD studies.

PY - 2019/11/18

Y1 - 2019/11/18

N2 - In the context of biorefinery for the production of chemicals, this study optimised the processes to enhance the anaerobic production of ethanol and short chain organic acids from glucose. The optimised variables included (with a total of 49 runs) residence time (2-100 h), temperature (25-35 OC), type of inoculum (soil or anaerobic digester), presence or absence of pre-acclimation of the inoculum, glucose concentration (5-50 g/L), continuous or batch mode and composition of the mineral media (mineral solution with or without trace elements, with deionised or tap water). In continuous experiments the residence time was the most important parameter that affected glucose conversion (over 80 % glucose conversion for residence time longer than 30 h) and product yields (ethanol was the main product in the range of residence times 20-50 h, with yields in the range 0.30-0.40 g/g glucose removed). Temperature, type of inoculum and pre-acclimation had little effect on glucose conversion and products yield. The addition of trace elements had an important beneficial effect on the removal of glucose when it was fed at the highest concentration (50 g/L) in both continuous and batch experiments. In batch runs acetate was generally the main fermentation product rather than ethanol and ethanol conversion into acetate was favoured by nitrogen sparging, probably due to the reduced hydrogen partial pressure.

AB - In the context of biorefinery for the production of chemicals, this study optimised the processes to enhance the anaerobic production of ethanol and short chain organic acids from glucose. The optimised variables included (with a total of 49 runs) residence time (2-100 h), temperature (25-35 OC), type of inoculum (soil or anaerobic digester), presence or absence of pre-acclimation of the inoculum, glucose concentration (5-50 g/L), continuous or batch mode and composition of the mineral media (mineral solution with or without trace elements, with deionised or tap water). In continuous experiments the residence time was the most important parameter that affected glucose conversion (over 80 % glucose conversion for residence time longer than 30 h) and product yields (ethanol was the main product in the range of residence times 20-50 h, with yields in the range 0.30-0.40 g/g glucose removed). Temperature, type of inoculum and pre-acclimation had little effect on glucose conversion and products yield. The addition of trace elements had an important beneficial effect on the removal of glucose when it was fed at the highest concentration (50 g/L) in both continuous and batch experiments. In batch runs acetate was generally the main fermentation product rather than ethanol and ethanol conversion into acetate was favoured by nitrogen sparging, probably due to the reduced hydrogen partial pressure.

KW - glucose

KW - anaerobic fermentation

KW - biorefinery

KW - organic acids

KW - ethanol

M3 - Article

JO - Biomass Conversion and Biorefinery

JF - Biomass Conversion and Biorefinery

SN - 2190-6815

ER -