Effects of dietary fatty acids on eicosanoid-generating capacity, fatty acid composition and chemotactic activity of rainbow trout (Oncorhynchus mykiss) leucocytes

Ian Ashton, Karen Clements, Susan E. Barrow, Christopher J. Secombes, Andrew F. Rowley*

*Corresponding author for this work

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Rainbow trout, Oncorhynchus mykiss, were maintained on isocalorific diets in which either sunflower, menhaden or Fosol oils were used as the dietary source of fatty acids. At intervals over a period of 6 months, head kidney leucocytes were isolated and used for the analysis of their fatty acid composition and eicosanoid-generating capacity. Major changes in fatty acid composition were apparent within 4 weeks on the diets, with fish fed sunflower oil diets showing a 2.1-fold increase in total n - 6 fatty acids and a 2.3-fold decrease in n - 3 fatty acids, compared with the original basal levels. By week 8 the fatty acid composition changes were greater in the sunflower-fed fish, but thereafter remained relatively stable to the end of the experiment at week 24. Leucocytes from the fish maintained for > 8 weeks on the sunflower oil containing diet produced significantly lower percentages of 5-series lipoxygenase products derived from eicosapentaenoic acid including 12-hydroxyeicosapentaenoic acid, leukotriene B5 and lipoxin A5 compared with those cells from fish fed either menhaden or Fosol based diets. Unlike the fatty acid composition, differences in lipoxygenase product profiles between the dietary groups increased throughout the experiment and by week 24 the arachidonic acid/eicosapentaenoic acid derived product ratios were approx. 14:1 in the sunflower oil-fed fish compared with approx. 1:1.5 in the menhaden oil-fed fish. A functional consequence of these differing ratios was seen in the ability of supernatants containing these products to cause the in vitro locomotion of trout neutrophils. Supematants from sunflower oil-fed fish were less chemo-attractive than supernatants from menhaden or Fosol oil-fed fish.

Original languageEnglish
Pages (from-to)253-262
Number of pages10
JournalBiochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
Volume1214
Issue number3
DOIs
Publication statusPublished - 6 Oct 1994

Fingerprint

Eicosanoids
Oncorhynchus mykiss
Fish
Fishes
Leukocytes
Fatty Acids
Nutrition
Chemical analysis
Diet
Eicosapentaenoic Acid
Lipoxygenase
Helianthus
Head Kidney
Arachidonate 5-Lipoxygenase
Fish Oils
Trout
Omega-3 Fatty Acids
Locomotion
Arachidonic Acid
Oils

Keywords

  • (O. mykiss)
  • (Rainbow trout)
  • Arachidonic acid
  • Chemotaxis
  • Fatty acid metabolism
  • Icosapentaenoic acid
  • Leucocyte
  • Leukotriene
  • Lipoxin
  • Lipoxygenase products

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Endocrinology

Cite this

@article{ef23943391094e2d9c72dcbd225614aa,
title = "Effects of dietary fatty acids on eicosanoid-generating capacity, fatty acid composition and chemotactic activity of rainbow trout (Oncorhynchus mykiss) leucocytes",
abstract = "Rainbow trout, Oncorhynchus mykiss, were maintained on isocalorific diets in which either sunflower, menhaden or Fosol oils were used as the dietary source of fatty acids. At intervals over a period of 6 months, head kidney leucocytes were isolated and used for the analysis of their fatty acid composition and eicosanoid-generating capacity. Major changes in fatty acid composition were apparent within 4 weeks on the diets, with fish fed sunflower oil diets showing a 2.1-fold increase in total n - 6 fatty acids and a 2.3-fold decrease in n - 3 fatty acids, compared with the original basal levels. By week 8 the fatty acid composition changes were greater in the sunflower-fed fish, but thereafter remained relatively stable to the end of the experiment at week 24. Leucocytes from the fish maintained for > 8 weeks on the sunflower oil containing diet produced significantly lower percentages of 5-series lipoxygenase products derived from eicosapentaenoic acid including 12-hydroxyeicosapentaenoic acid, leukotriene B5 and lipoxin A5 compared with those cells from fish fed either menhaden or Fosol based diets. Unlike the fatty acid composition, differences in lipoxygenase product profiles between the dietary groups increased throughout the experiment and by week 24 the arachidonic acid/eicosapentaenoic acid derived product ratios were approx. 14:1 in the sunflower oil-fed fish compared with approx. 1:1.5 in the menhaden oil-fed fish. A functional consequence of these differing ratios was seen in the ability of supernatants containing these products to cause the in vitro locomotion of trout neutrophils. Supematants from sunflower oil-fed fish were less chemo-attractive than supernatants from menhaden or Fosol oil-fed fish.",
keywords = "(O. mykiss), (Rainbow trout), Arachidonic acid, Chemotaxis, Fatty acid metabolism, Icosapentaenoic acid, Leucocyte, Leukotriene, Lipoxin, Lipoxygenase products",
author = "Ian Ashton and Karen Clements and Barrow, {Susan E.} and Secombes, {Christopher J.} and Rowley, {Andrew F.}",
year = "1994",
month = "10",
day = "6",
doi = "10.1016/0005-2760(94)90071-X",
language = "English",
volume = "1214",
pages = "253--262",
journal = "Biochimica et Biophysica Acta - Lipids and Lipid Metabolism",
issn = "0005-2760",
publisher = "Elsevier BV",
number = "3",

}

TY - JOUR

T1 - Effects of dietary fatty acids on eicosanoid-generating capacity, fatty acid composition and chemotactic activity of rainbow trout (Oncorhynchus mykiss) leucocytes

AU - Ashton, Ian

AU - Clements, Karen

AU - Barrow, Susan E.

AU - Secombes, Christopher J.

AU - Rowley, Andrew F.

PY - 1994/10/6

Y1 - 1994/10/6

N2 - Rainbow trout, Oncorhynchus mykiss, were maintained on isocalorific diets in which either sunflower, menhaden or Fosol oils were used as the dietary source of fatty acids. At intervals over a period of 6 months, head kidney leucocytes were isolated and used for the analysis of their fatty acid composition and eicosanoid-generating capacity. Major changes in fatty acid composition were apparent within 4 weeks on the diets, with fish fed sunflower oil diets showing a 2.1-fold increase in total n - 6 fatty acids and a 2.3-fold decrease in n - 3 fatty acids, compared with the original basal levels. By week 8 the fatty acid composition changes were greater in the sunflower-fed fish, but thereafter remained relatively stable to the end of the experiment at week 24. Leucocytes from the fish maintained for > 8 weeks on the sunflower oil containing diet produced significantly lower percentages of 5-series lipoxygenase products derived from eicosapentaenoic acid including 12-hydroxyeicosapentaenoic acid, leukotriene B5 and lipoxin A5 compared with those cells from fish fed either menhaden or Fosol based diets. Unlike the fatty acid composition, differences in lipoxygenase product profiles between the dietary groups increased throughout the experiment and by week 24 the arachidonic acid/eicosapentaenoic acid derived product ratios were approx. 14:1 in the sunflower oil-fed fish compared with approx. 1:1.5 in the menhaden oil-fed fish. A functional consequence of these differing ratios was seen in the ability of supernatants containing these products to cause the in vitro locomotion of trout neutrophils. Supematants from sunflower oil-fed fish were less chemo-attractive than supernatants from menhaden or Fosol oil-fed fish.

AB - Rainbow trout, Oncorhynchus mykiss, were maintained on isocalorific diets in which either sunflower, menhaden or Fosol oils were used as the dietary source of fatty acids. At intervals over a period of 6 months, head kidney leucocytes were isolated and used for the analysis of their fatty acid composition and eicosanoid-generating capacity. Major changes in fatty acid composition were apparent within 4 weeks on the diets, with fish fed sunflower oil diets showing a 2.1-fold increase in total n - 6 fatty acids and a 2.3-fold decrease in n - 3 fatty acids, compared with the original basal levels. By week 8 the fatty acid composition changes were greater in the sunflower-fed fish, but thereafter remained relatively stable to the end of the experiment at week 24. Leucocytes from the fish maintained for > 8 weeks on the sunflower oil containing diet produced significantly lower percentages of 5-series lipoxygenase products derived from eicosapentaenoic acid including 12-hydroxyeicosapentaenoic acid, leukotriene B5 and lipoxin A5 compared with those cells from fish fed either menhaden or Fosol based diets. Unlike the fatty acid composition, differences in lipoxygenase product profiles between the dietary groups increased throughout the experiment and by week 24 the arachidonic acid/eicosapentaenoic acid derived product ratios were approx. 14:1 in the sunflower oil-fed fish compared with approx. 1:1.5 in the menhaden oil-fed fish. A functional consequence of these differing ratios was seen in the ability of supernatants containing these products to cause the in vitro locomotion of trout neutrophils. Supematants from sunflower oil-fed fish were less chemo-attractive than supernatants from menhaden or Fosol oil-fed fish.

KW - (O. mykiss)

KW - (Rainbow trout)

KW - Arachidonic acid

KW - Chemotaxis

KW - Fatty acid metabolism

KW - Icosapentaenoic acid

KW - Leucocyte

KW - Leukotriene

KW - Lipoxin

KW - Lipoxygenase products

UR - http://www.scopus.com/inward/record.url?scp=0028061583&partnerID=8YFLogxK

U2 - 10.1016/0005-2760(94)90071-X

DO - 10.1016/0005-2760(94)90071-X

M3 - Article

VL - 1214

SP - 253

EP - 262

JO - Biochimica et Biophysica Acta - Lipids and Lipid Metabolism

JF - Biochimica et Biophysica Acta - Lipids and Lipid Metabolism

SN - 0005-2760

IS - 3

ER -