Effects of esculin and esculetin on the survival of Escherichia coli O157 in human faecal slurries, continuous-flow simulations of the rumen and colon and in calves

S. H. Duncan, E. Carol McWilliam Leitch, K N Stanley, Anthony James Richardson, R A Laven, Harry James Flint, C. S. Stewart

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The human pathogen Escherichia coli O157:H7 is thought to be spread by direct or indirect contact with infected animal or human faeces. The present study investigated the effects of the plant coumarin esculin and its aglycone esculetin on the survival of a strain of E. coli O157 under gut conditions. The addition of these compounds to human faecal slurries and in vitro continuous-flow fermenter models simulating conditions in the human colon and rumen caused marked decreases in the survival of an introduced strain of E. coli O157. When four calves were experimentally infected with E. coli O157 and fed esculin, the pathogen was detected in five of twenty-eight (18%) of faecal samples examined post-inoculation, compared with thirteen of thirty-five (37%) of faecal samples examined from five control calves not fed esculin. Coumarin compounds that occur naturally in dietary plants or when supplemented in the diet probably inhibit the survival of E. coli O157 in the gut.

Original languageEnglish
Pages (from-to)749-755
Number of pages7
JournalBritish Journal of Nutrition
Volume91
Issue number5
DOIs
Publication statusPublished - May 2004

Keywords

  • plant coumarins
  • Escherichia coli O157
  • rumen
  • colon
  • hemolytic-uremic syndrome
  • volatile fatty acids
  • 16S ribosomal-RNA
  • continuous culture
  • essential oils
  • gut bacteria
  • cattle
  • fermentation
  • infections
  • resistance

Cite this

Effects of esculin and esculetin on the survival of Escherichia coli O157 in human faecal slurries, continuous-flow simulations of the rumen and colon and in calves. / Duncan, S. H.; Leitch, E. Carol McWilliam; Stanley, K N ; Richardson, Anthony James; Laven, R A ; Flint, Harry James; Stewart, C. S.

In: British Journal of Nutrition, Vol. 91, No. 5, 05.2004, p. 749-755.

Research output: Contribution to journalArticle

Duncan, S. H. ; Leitch, E. Carol McWilliam ; Stanley, K N ; Richardson, Anthony James ; Laven, R A ; Flint, Harry James ; Stewart, C. S. / Effects of esculin and esculetin on the survival of Escherichia coli O157 in human faecal slurries, continuous-flow simulations of the rumen and colon and in calves. In: British Journal of Nutrition. 2004 ; Vol. 91, No. 5. pp. 749-755.
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AU - Richardson, Anthony James

AU - Laven, R A

AU - Flint, Harry James

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AB - The human pathogen Escherichia coli O157:H7 is thought to be spread by direct or indirect contact with infected animal or human faeces. The present study investigated the effects of the plant coumarin esculin and its aglycone esculetin on the survival of a strain of E. coli O157 under gut conditions. The addition of these compounds to human faecal slurries and in vitro continuous-flow fermenter models simulating conditions in the human colon and rumen caused marked decreases in the survival of an introduced strain of E. coli O157. When four calves were experimentally infected with E. coli O157 and fed esculin, the pathogen was detected in five of twenty-eight (18%) of faecal samples examined post-inoculation, compared with thirteen of thirty-five (37%) of faecal samples examined from five control calves not fed esculin. Coumarin compounds that occur naturally in dietary plants or when supplemented in the diet probably inhibit the survival of E. coli O157 in the gut.

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KW - continuous culture

KW - essential oils

KW - gut bacteria

KW - cattle

KW - fermentation

KW - infections

KW - resistance

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